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Citations for Agilent :
1151 - 1173 of 1173 citations for Recombinant Human CSH1 CSH2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were processed as described and probed with a set of 258 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were incubated at room temperature for 20 minutes and protein content was measured using a spectrophotometer (Model: Cary 60 UV-Vis, Agilent Technologies, CA, USA) at 595 nm ...
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bioRxiv - Physiology 2024Quote: ... To analyze the deuterium enrichment of alanine in the protein fractions we used Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA)52,53.
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bioRxiv - Cancer Biology 2023Quote: ... sections were washed with tris buffered saline with tween (TBST) for 10 min before unspecific protein binding was blocked using 10 % goat serum (Dako, X0907, CA, USA) in 3 % bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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bioRxiv - Microbiology 2019Quote: ... Desalted tryptic peptides corresponding to 1 μg of protein were loaded for LC-MS/MS analysis with an Agilent 1100 Capillary LC system (Agilent Technologies, San Jose, CA) and run on a Q Exactive mass spectrometer (ThermoFisher Scientific Inc.) ...
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bioRxiv - Immunology 2022Quote: ... Total IgG Abs were affinity-captured from plasma or sera using a Protein G AssayMAP Cartridge Rack on the Bravo (Agilent Technologies, Santa Clara, CA) or Protein G Sepharose 4 Fast Flow beads (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Cancer Biology 2022Quote: Pan-HLA Class I-specific antibody (clone W6/32) was coupled to Protein-A resin packed into AssayMAP Bravo compatible large capacity cartridges (PA-W 25 μL) (Agilent, Part number G5496-60018). The coupled antibodies were then crosslinked with 20 mM Dimethyl pimelimidate dihydrochloride (DMP ...
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bioRxiv - Biophysics 2024Quote: Accurate masses of unlabeled and labeled proteins were determined via LC-MS with an Agilent PL1912- 1300 reverse phase HPLC column (Agilent, Santa Clara, CA, USA) connected to an AB Sciex 5600 QTOF mass spectrometer (AB Sciex ...
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bioRxiv - Biochemistry 2022Quote: ... the UV-visual absorbance spectra of conjugated proteins were recorded in the range 200–700 nm using Agilent Cary 100 absorbance spectrophotometer (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Biochemistry 2020Quote: ... Selenomethionine (SeMet)-labeled and native proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RP-X and BL21-CodonPlus (DE3)-RIL (Agilent Technologies, Santa Clara, CA, USA), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were examined in a detergent insolubility assay adapted from Drisaldi et al.43 and the proteins in the supernatant and pellet were analyzed by Western blot using the anti-tau rabbit polyclonal antibody DAKO (Agilent Technologies Italy SpA, Milan, Italy) (1:10000 dilution) ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Immunology 2021Quote: ... 4 μg of deglycosylated protein was used for each injection on the LC-MS system to acquire intact MS signal after separation of protease and protein by LC (Agilent PLRP-S reversed phase column). Thermo MS (Q Exactive Plus Orbitrap ...
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bioRxiv - Immunology 2023Quote: ... Protein intact mass was determined using a 1969 MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc. – Palo Alto, CA, USA). The instrument was configured with the standard ESI source and operated in positive ion mode ...
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bioRxiv - Microbiology 2023Quote: ... Protein intact mass was determined using a 1969 MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc. – Palo Alto, CA, USA). The instrument was configured with the standard ESI source and operated in positive ion mode ...
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bioRxiv - Neuroscience 2023Quote: ... Protein intact mass was determined using a 1969 MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc. – Palo Alto, CA, USA). The instrument was configured with the standard ESI source and operated in positive ion mode ...
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bioRxiv - Cancer Biology 2023Quote: ... All oligo- conjugated antibodies were generated as previously described and were run on a Bioanalyzer Protein 230 electrophoresis chip (Agilent Technologies, cat. no 5067-1517) to verify successful conjugation6.
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bioRxiv - Physiology 2024Quote: ... To analyze the deuterium enrichment of alanine in the protein fractions we used Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA)52,53.
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bioRxiv - Microbiology 2020Quote: ... For intact protein analysis,10 μL of desalted protein (∼1 mg/ml) was injected onto a Zorbax 300Å Stable Bond C8 column (Agilent Technologies, 4.6 x 50 mm, 3.5 μM) for reversed phase separation at 60°C and 0.4 mL/min ...