Labshake search
Citations for Agilent :
1151 - 1200 of 1988 citations for 7 METHYLTHIENO 2 3 B QUINOLINE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-p53 (Clone DO-7) (1:1000, #M7001, DAKO, Agilent, Santa Clara, California, USA) and mouse anti-beta Actin (Clone C4 ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-p53 (Clone DO-7) (1:1000, #M7001, DAKO, Agilent, Santa Clara, California, USA) and mouse anti-beta Actin (Clone C4 ...
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bioRxiv - Pathology 2024Quote: ... Antigen retrieval was carried out using Proteinase K (5 μg/ml, 7 mins, Dako, UK). Sections were rinsed in running tap water for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: 3*10^3 of HPLFs per well were seeded into Seahorse XFe96 well plate (Agilent Technologies, 200941) for overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality of all samples (n=7 time points and n=3 experiments from independent passages) was evaluated by both Nanodrop for (A260/280 >2) and Bioanalyzer 2100 High Sensitivity RNA Analysis chips (Agilent, Cat. 5067-1513) which displayed intact RNA integrity (RIN >9) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Biochemistry 2019Quote: ... in 200μl of dichloromethane containing 200 ng of 2-tetradecyl acetate (internal standard) in 2ml glass vials with polytetrafluoroethylene-coated caps (Agilent, Santa Clara, California). After one hour ...
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bioRxiv - Cell Biology 2019Quote: ... and then probed with primary antibody (see Supp. Table 2) followed by the appropriate HRP-conjugated secondary antibody (1:5000) (Agilent, Santa Clara, CA).
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bioRxiv - Genomics 2020Quote: ... Chromium Genome Reagents Kit Version 2 User Guide) and size and concentration determined using an Agilent 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies, CA, USA). Libraries were then sequenced on an Illumina NovaSeq 6000 System following the manufacturer’s protocols (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunofluorescence staining was then performed using antibodies to detect CD31 (dilution 1:250 ...
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
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bioRxiv - Genetics 2022Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Neuroscience 2020Quote: ... and heat-induced antigen retrieval was performed using 10mM sodium citrate solution (pH 6.0, pSer129) or citrate Target Retrieval Solution (Dako #S169984-2, pH 6.1) with 0.05% Tween-20 (conformation-specific ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Synthetic Biology 2020Quote: ... After hybridisation microarray slides were washed with Gene Expression Wash Buffer 1 and Gene Expression Wash Buffer 2 (Agilent, Cat No. 5188-5327) with added Triton X-102 (0.005% final conc. ...
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bioRxiv - Biochemistry 2021Quote: ... and 12 were used as primary detection against T22 capture antibody) was an anti-tau polyclonal rabbit antibody (A002401-2) purchased from Agilent (Santa Clara, CA). A goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... followed by sample measurements, all performed in duplicates using the OT-2 robot (Opentrons, Long Island City, NY) and BioTek Synergy (Agilent Technologies, Waldbronn, Germany). The acquired data were evaluated using the Shiny application based on the determined standard curves for accurate quantification [62].
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bioRxiv - Microbiology 2024Quote: ... The growth was monitored by measuring the optical density at 600 nm (OD600) in an Epoch 2 plate reader (Agilent, Santa Clara, CA). The growth rates were calculated using the R package growthcurver (32).
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cal27 were seeded at a density of 2×104 cells per well in a Seahorse XFe24 Cell Culture Microplate (102340-100; Agilent, Santa Clara, CA). Cells were incubated overnight for adherence in a culture media composed of DMEM and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Pathology 2024Quote: The samples were resuspended in 10µL phase A of Q-TOF (2% ACN, 0.1% FA in water HPLC grade) in mass spectrometry polypropylene vials (Agilent technologies, reference 5190-3155). The samples (1µL out of 10µL ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies against HER-2 (cell signaling, #4290T, 1:200) or EGFR (cell signaling, #4267, 1:50) and αSMA (Agilent, M085129, 1:100) overnight at +4C ...
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bioRxiv - Biochemistry 2024Quote: ... Assay plates were generated by plating 2 µL of the deep well stock plates into 384-well assay plates (Thermo Scientific #142761 using the Agilent V11 Bravo). 88 µL of SrEpac cultured in high-nutrient media (5% Sea Water Complete ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako ...
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bioRxiv - Cancer Biology 2019Quote: ... Capture was performed according to the manufacturer’s instructions using an NGS Workstation Option B (Agilent) for automated library preparation starting with 3 µg DNA per sample ...
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bioRxiv - Microbiology 2021Quote: ... The overlap PCR product was cloned into pSC-B ampkan (Agilent Technologies, La Jolla, CA) to generate pScGFPTUB1 using a Stratagene blunt PCR cloning kit (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cholesterol was quantified and isotope labeling pattern analyzed using Mass Hunter B.06.00 software (Agilent). Cholesterol and lathosterol internal standard peak areas were extracted from mass-to-charge ratio (m/z ...
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bioRxiv - Plant Biology 2020Quote: ... Data acquisition and processing was done with Mass Hunter software version B.08 (Agilent Technologies).
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bioRxiv - Plant Biology 2021Quote: ... Solvent A and B were supplied with 0.1% (v/v) Infinity Lab deactivator additive (Agilent) to improve peak shapes ...
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bioRxiv - Microbiology 2019Quote: Data were analysed using MassHunter Qualitative and Quantitative Analysis software (version B.07.00, Agilent Technologies). Identification of metabolites was performed in accordance with metabolite identification (Metabolomics Standard Initiative ...
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bioRxiv - Biochemistry 2022Quote: ... Metabolite identification and quantification was performed using MassHunter Workstation software (B.06.00 SP01, Agilent Technologies) by comparison to the retention times ...
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bioRxiv - Microbiology 2019Quote: ... followed by 5 minutes of re-equilibration at 0% B (Agilent 1290 Infinity LC system). Post-column ...
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bioRxiv - Biochemistry 2019Quote: ... The peak integrals were obtained using the MassHunter Qualitative Navigator B.08.00 software (Agilent, USA)10 ...
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bioRxiv - Immunology 2021Quote: ... GC-APCI-MS analysis was carried out on an Agilent 7890 B gas chromatograph (Agilent) coupled to an Impact II quadrupole time-of-flight (Q-TOF ...
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bioRxiv - Cancer Biology 2022Quote: Raw data files (.d) were processed using Profinder B.08.00 SP3 software (Agilent Technologies, CA) with an in-house database containing retention time and accurate mass information on 600 standards from Mass Spectrometry Metabolite Library (IROA Technologies ...