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Citations for Agilent :
1101 - 1150 of 5405 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Coverslips were mounted with Dako Fluorescent Mounting Media (Agilent S302380-2) and allowed to dry ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were coverslipped using fluorescence mounting media (CatNo. S302380-2, Agilent). Additionally ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended by 200 μL 1% (w/v) BSA in PBS for detection by a NovoCyte flow cytometry (Agilent).
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
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bioRxiv - Physiology 2019Quote: The oxygen consumption rate (OCR) of HL-1 cardiomyocytes was measured using the XFp Cell Mito Stress Test by Agilent Seahorse24 ...
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bioRxiv - Systems Biology 2020Quote: ... lymphatic tissue was cut into 1-mm pieces with a sterile scalpel and disaggregated mechanically into a single-cell suspension using the Medimachine (DAKO) equipped with sterile cartridges holding rotating knives (Medicons ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Genomics 2019Quote: ... The sheared DNA was used to prepare indexed whole exome sequencing libraries using the Agilent SureSelect XT Human all exon v6 + UTR kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into exome sequencing libraries using the SureSelect Human All Exon V7 Exome kit (Agilent). DNA samples were pooled to achieve an on-bait depth of 100× for the buffy coat gDNA and 300× for the plasma cfDNA.
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bioRxiv - Genomics 2019Quote: ... WES libraries were prepared using the SeqCap EZ Exome Kit v3 (NimbleGen) or the SureSelect Human All Exon V7 Low Input Exome kit (Agilent). Equimolar pooled libraries were sequenced on HiSeq 2000 and 4000 instruments (Illumina ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed using SureSelect Human All Exon Kit v5 (cat# 5990-9857EN, Agilent Technologies, Santa Clara, CA, USA), sequencing was performed on the Illumina Xten platform ...
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bioRxiv - Microbiology 2021Quote: Qualitative and quantitative measurements of viral load were determined by quantitative RT-PCR,10 ul of RNA combined with 25 ng Human Universal Reference RNA (Agilent) was purified by PureBeads (Roche Sequencing) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 – 200 ng of DNA underwent library preparation and exome capture using the SureSelectXT low input protocol with Human All Exon V7 (Agilent) as per manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Genomics 2020Quote: ... The BAMfile used for IGV visualization was generated from WES data obtained with the SureSelect Human All Exon V6 kit (exome capture kit) from Agilent. Group alignment is by chromosome of mate ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome regions were captured by an Agilent SureSelect Human All Exon V4 Kit and V5+ LincRNA (Agilent Technologies, CA, USA). Sequencing was performed on an Illumina HiSeq 2000 instrument (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries and hybridization capture were performed following the SureSelectXT HS recommended protocol with SureSelect Human All Exon V6+UTR baits (Agilent). Indexed libraries were sequenced on an Illumina NovaSeq with the S4 flow cell (Illumina ...
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bioRxiv - Genomics 2022Quote: ... the three pools were spiked in a diagnostic WES workflow containing 186 exomes (SureSelectXT Low Input Target Enrichment System, Human All Exon V7 probes, Bravo Automated Liquid Handling Platform, Agilent), and 2 whole genome preparations (NEXTFLEX Rapid XP DNA-seq kit ...
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bioRxiv - Neuroscience 2022Quote: ... Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies, pH 9.0) under heat ...
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bioRxiv - Neuroscience 2021Quote: Exonic sequences were enriched with the SureSelect Human All Exon 50□Mb V5 Kit (Agilent Technologies, Santa Clara, CA, USA). Sequences were generated on a HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Each RNA sample was processed with fluorescence-labelling to prepare complimentary RNA (cRNA) targets that could be detected by an SBC human ceRNA array V1.0 (4□×□180K, Agilent Technologies). The microarray kit contained 88,371 circRNA probes and 18,853 mRNA probes ...
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bioRxiv - Cancer Biology 2019Quote: ... Exome capture and sequencing library preparation were performed using Agilent SureSelect Human All Exon V5 (Agilent Genomics, Santa Clara, CA), following standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A, RIOK2123A,246A) were created using site directed mutagenesis (Lightning Quick Change Kit, Agilent) to make alanine substitutions at catalytic residues Lys-123 and Asp-246 in both human RIOK2 and dRIOK2 as described by other groups (22 ...
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bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
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bioRxiv - Cancer Biology 2022Quote: ... WES libraries were generated by exome capture of approximately 20,000 coding genes using SureSelect human All exon V6 kit (Agilent Technologies) and paired-end sequencing was performed on a HiSeq sequencer (Illumina ...
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bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... For cDNA expression microarray analysis using SurePrint G3 Human GE 8×60K Ver.2.0 (Agilent Technologies, Santa Clara, CA, USA), total RNA was sent to a commercial service (Hokkaido System Science ...
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Genomics 2019Quote: The FLNC reads from the Isoseq3 refine step were error corrected using Lordec (-k 31 -s 3) with short read RNAseq data from the Universal Human Reference RNA (Agilent) (https://www.ncbi.nlm.nih.gov/sra/SRX1426160 ...
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bioRxiv - Cell Biology 2019Quote: ... total leukocyte DNA was enriched using the Agilent SureSelect Human All Exon 50 Mb capture kit (Agilent Technologies, SantaClara, CA). Bidirectional 100-bp paired-end sequencing (Illumina Hi-Seq 2000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Quantitative determination of diphenhydramine in human plasma was accomplished by the use of high-performance liquid chromatography with tandem mass spectrometry detection (Agilent Series 1100 LC with MS detector API4000 ...
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... exome enrichment was performed using the SureSelect Human All Exon 50 Mb Kit V5 (Agilent Technologies, Santa Clara, CA, USA). Sequencing was done on a HiSeq4000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... Hybrid capture of exonic regions was performed using Agilent SureSelect in-solution capture reagents and SureSelect XT Human All Exon V4 probes (Agilent). Captured libraries were sequenced using 100 bp paired-end runs on Illumina HiSeq 2500 ...
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bioRxiv - Cancer Biology 2023Quote: ... exome capture was performed on 200 ng DNA using a customised version of the Agilent Human All Exome V5 Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Immunology 2024Quote: To accurately investigate the bioenergetic function of bovine and human MØ with an extracellular flux analyser (Seahorse Bioscience, Inc, USA), both cell types were characterized according to the manufacturer’s recommended basal and test conditions (23).
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...