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Citations for Agilent :
1101 - 1150 of 3885 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Physiology 2023Quote: ... during which lipids were separated on an Eclipse XDB C18 5-μm column (i.d. 150 × 4.6 mm; Agilent Technologies) using an isocratic mobile phase comprising chloroform:methanol (1:1 v/v ...
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bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Colonies were stained with Wright-Giesma and plates were scanned using the Biotek Cytation 5 Imaging Multimodal Reader (Agilent). From scanned images ...
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bioRxiv - Synthetic Biology 2023Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... used a two solvent gradient (Mobile Phase A: 10 mM Ammonium Acetate in 90/10 Water/Acetonitrile, pH 9.2, 5 μm Agilent InfinityLab Deactivator Additive ...
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bioRxiv - Plant Biology 2024Quote: ... The organic phase was dried using anhydrous MgSO4 and analysed on GC-2010 Pro gas chromatograph (Shimadzu, Japan) equipped with an HP-5MS 30 m × 0.25 mm (5%-Phenyl)-methylpolysiloxane column (19091S-133, Agilent) with nitrogen as the carrier gas with splitless injection mode ...
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bioRxiv - Immunology 2022Quote: ... The HPLC system consisted of a Thermo Scientific Ultimate 3000 equipped with a UV detector set at 274 nm and a reverse phase Zorbax 300SB-C18 column (4.6 × 250 mm i.d., pore size 5 μm Agilent, U.S.A.). The mobile phase consisted of a 50/50 acetonitrile/buffer (0,02 % ammonium acetate solution at pH 5 ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Microarray images were collected by using sure scan microarray scanner system at 5 μm resolution (Agilent Technologies, Scanner 2600D). Fluorescent intensities were quantified using feature extraction software ...
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bioRxiv - Plant Biology 2022Quote: ... and 1 mL injections (passed through silica to filter out precipitate following derivatization) on a Zorbax RX-silica (250 x 4.6 mm, 5 µm; Agilent) column and a mobile phase consisting of solvent A (100% hexanes ...
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bioRxiv - Genomics 2022Quote: ... The GC was equipped with a 30 m DB-35MS + 5 m DuraGuard capillary column (0.25 mm inner diameter, 0.25 μm film thickness; Agilent Technologies). Helium was used as carrier gas at a flow rate of 1.0 mL min−1 and the GC-oven was run at following temperatures and times per sample ...
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bioRxiv - Microbiology 2023Quote: ... of the in vitro SAM cleavage reaction were separated by reverse-phase high performance liquid chromatography (HPLC) with Zorbax Eclipse Plus C18 5-μm (4.6 by 250 mm) column (Agilent) connected to the 1220 Infinity II LC system (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Using a LC-10AT HPLC instrument (Shimadzu), the pooled sample was loaded onto a ZORBAX Eclipse XDB C18 column (5 μm, 4.6 × 150 mm) (Agilent) that had been equilibrated with 25 mM sodium acetate ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 minutes and incubated with the primary antibody of interest which was prepared in antibody diluent (S0809, Dako). Slides were put in a humidified chamber at 4C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... using a HP-5MSI 5% phenyl methyl silox with 30 m × 250 µM × 0.25 µm film thickness (Agilent Technologies). Identification and quantification were conducted using AMDIS (Automated Mass spectral Deconvolution and Identification System ...
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bioRxiv - Physiology 2024Quote: ... and injected 100 µL of sample into an Agilent 1100 series HPLC fitted with a Zorbax RX-SIL column (4.6x25 mm, 5 µm, Agilent). Elution of samples was achieved by a gradient mobile phase of 0.5% ethyl acetate in hexane for 5 minutes then increased to 10% ethyl acetate in hexane for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... As species were separated by ion-pairing chromatography using a Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm particle size, Agilent) through isocratic elution for 25 min with the mobile phase used for extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... The material was loaded on to a Zorbax 2.1 mm x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Physiology 2024Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described37 ...
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bioRxiv - Cancer Biology 2024Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Microbiology 2024Quote: ... reverse-phase LC was performed on a Zorbax Eclipse XDB-C8 column (150 mm, 4.6 mm, 5 µm; Agilent) using Shimadzu UFLC XR instrument ...
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bioRxiv - Microbiology 2024Quote: ... Incubation was carried out at 30 °C in a plate reader (BioTek Cytation 5; Agilent, Santa Clara, CA, USA) and the data were recorded using Biotek Gen5 software (Agilent) ...
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bioRxiv - Systems Biology 2024Quote: ... were directly injected using a Shimazu LC-40 UHPLC onto a 5 cm x 2.1 mm C18 column analytical column (Agilent InfinityLab Poroshell 120 EC-C18 ...
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bioRxiv - Genomics 2024Quote: ... Each protein sample was injected onto an Poroshell 300SB-C8 column (2.1 x 75 mm, 5 µM, room temp, Agilent) and separated using a linear gradient from 5% acetonitrile for 0 to 2 min and ramping to 95% acetonitrile over 7.5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were Hoechst-stained and relative cell numbers counted using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent) at the specific timepoints.
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bioRxiv - Biophysics 2024Quote: ... in xylene and deposited with a 12 μm inter-fiber spacing on top of large diameter (2 μm) support fibers prepared from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Developmental Biology 2024Quote: ... Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies; CatNo. 200521-5). Primers used are shown in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated for 30 minutes in 5 mM MβCD in fresh XFp medium (Agilent Technologies, Santa Clara, CA). To interrogate mitochondrial function ...
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Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomersbioRxiv - Microbiology 2024Quote: ... internally coated with a 0.18 µm film composed of a non-polar stationary phase containing 5% diphenyl-polysiloxane (HP5-MS, Agilent). The injection was performed in split-less mode at 280°C ...
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bioRxiv - Microbiology 2024Quote: Content of the rRNA hydrolysate was resolved using Zorbax SB-C18 column (5 µm, 0.5 x 150 mm, Agilent) and the LC conditions were tuned empirically ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed 2 x 5 min in PBS-T and incubated with streptavidin–horseradish peroxidase complex (DakoCytomation). Following 2 x 5 min washes in PBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Specified gradients for HPLC purifications (MeCN in 0.2% TFA (aq.)) were linear (5 mL/min for 12 min (Agilent) or 25 mL/min for 10 min (Waters)) ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence of samples was measured at 25 °C using Cytation 5 Cell Imaging Multimode Reader (Agilent Technologies, USA). The mean fluorescence intensities (in arbitrary units ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For phthalates and BPA extraction we used Agilent Vac Elut Manifold with SPE cartridge C18 ODS 3 ml tubes 200 mg (Agilent Santa Clara CA, USA). SPE cartridges were conditioned with 2 ml of methanol and then 2 ml of MilliQ water ...
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bioRxiv - Synthetic Biology 2023Quote: Samples (3 µL) were injected into an ion mobility drift tube-quadrupole time-of-flight mass spectrometer (LC-IM-QTOF, Agilent 1290 series LC 6560). An Agilent Poroshell 120 HILIC-Z 150 × 2.1 mm ...
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bioRxiv - Neuroscience 2022Quote: ... samples were suspended in a 4M urea buffer containing ampholytes and subjected to off-gel IEF fractionation using a 24 well strip (pH 3-10) (Agilent, 3100 Off- gel fractionator). Following IEF ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8×60K V2 Arrays (SurePrint G3 Human Gene Expression 8×60K v2 Microarray Kit ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). 10-12 day old i3Neurons were treated with 5 nM vincristine ...
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bioRxiv - Immunology 2024Quote: ... turbidity was monitored in 2-minute intervals at 600 nm absorption using a Cytation 3 plate reader (BioTek, Agilent Technologies, Santa Barbara, CA, USA) preheated to 37 °C and after equilibrating for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP, 1:400, Dako; rabbit anti-Ki67 ...
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bioRxiv - Neuroscience 2023Quote: ... 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10, 1:500; CD68, DAKO A/S PG-M1 ...
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bioRxiv - Neuroscience 2024Quote: ... Every fourth section from approximately bregma −1.40 mm to −2.00 mm were immunostained with ionized calcium binding adaptor molecule 1 (Iba1; 1:2000; FUJIFILM Wako) and glial fibrillary acidic protein (GFAP; 1:1000; Dako); 4-6 sections per brain ...