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Citations for Agilent :
1101 - 1150 of 2118 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... slides were washed three times in PBS for 5 minutes and mounted in DAKO fluorescence mounting medium (Agilent).
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bioRxiv - Microbiology 2022Quote: ... Folsom, CA, USA), with an HP-5MS (30 m × 0.25 mm, 0.25 μm 5% diphenyl–95% dimethylpolysiloxane) (Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Samples of labeled and unlabeled antibody (5 – 10 μg) were run on a HPLC (Agilent 1260 Infinity II) using an Agilent AdvanceBIO SEC 300Å 2.7 mm column (PL1580-3301 ...
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bioRxiv - Cell Biology 2024Quote: ... the plate containing the follicles was transferred to a live-cell imaging system (Agilent BioTek Cytation 5, USA) to capture images at 6-minute intervals during ovulation ...
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bioRxiv - Bioengineering 2024Quote: ... and the number of cells that were alive or dead were quantified using Gen 5 software (Agilent Technologies).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Zorbax SB300-C18/5 μm particles and SPEC-PTC18/SPEC-PTSCX cleanup pipette tips were purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2023Quote: ... and a 5 m guard capillary column coupled to an Agilent 5975C inert mass selective detector (Agilent Technologies). Electron impact ionization (EI ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphopeptide enrichment was performed on 5 µL phase Fe(III)– NTA cartridges on an AssayMAP Bravo platform (Agilent) following the immobilized metal affinity chromatography protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... formic acid at a flow rate of 1.0 mL/min equipped with a ZORBAX SB-C18 column (4.6 × 250 mm, 5 μm, Agilent). Quantification was performed using standard calibration curves prepared using a series of known concentrations of the standard ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Some of them were subjected to further purification using semipreparative techniques (Agilent, C18, 5 μm, 250 × 10 mm) using appropriately selected aqueous AcN or MeOH solvent systems.
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bioRxiv - Biochemistry 2023Quote: ... HPLC was performed on an Agilent 1260 infinity II, equipped with a C-18 column (Mackerey-Nagel, 5 µm, ref 760.100.40) using acetonitrile (HPLC grade, Agilent) and ddH2O (0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was thoroughly washed 5 times with PBS-T and 100 μL of TMB-Blue substrate (DAKO) was added followed by incubation in the dark for 5-10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-μm sections were stained with antibodies or control IgG using the EnVision+System-HRP (DAB) kit (Dako North America Inc ...
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bioRxiv - Plant Biology 2022Quote: ... All samples were loaded into a 10 cm capillary column packed with 5 μM Zorbax SB-C18 (Agilent) and then connected to a 5 cm-long strong cationic exchange (SCX ...
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bioRxiv - Molecular Biology 2022Quote: pKS-SacB used for cloning in vitro MMEJ products (Figure 5) is a derivative of pBluescriptII KS(+) (Agilent), which was constructed by PCR amplifying the sacB gene of pACRISPR (Addgene plasmid #113348 ...
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bioRxiv - Zoology 2022Quote: ... and then into a C18 reversed-phase home-packed 15 cm column (SB-C18, ZORBAX, 5 micron, Agilent). Flow rate was maintained at 400 nL/min for a 60-min LC gradient ...
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bioRxiv - Microbiology 2022Quote: ... injection volume 5-10 μL and data was collected in centroid mode with Mass Hunter Workstation software (Agilent). Raw Agilent.d files were converted to mzXML with MSconvert and analysed using the Maven software package (84) ...
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bioRxiv - Genetics 2023Quote: ... slides were carefully rinsed 3 × 5 min with PBS and slides were mounted with Fluorescent Mounting Medium (Dako) and examined under fluorescence using a Zeiss microscope equipped with an AxioCam HRm camera.
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bioRxiv - Biochemistry 2023Quote: ... Peptide purity was assessed with an analytical column (Agilent, ZORBAX 300SB-C18, 4.6 x 150 mm, 5 μm) at a flow rate of 1 mL/min over a 0-90% B gradient in 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... equipped with a guard column (EC-C18; 3.0 mm internal diameter, 5 mm length, 2.7 μm particle size; Agilent), was conditioned for 20 minutes with 95 % mobile phase A and 5 % B ...
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bioRxiv - Cancer Biology 2023Quote: ... on a HPLC column (PLRP-S 1000 Å, 5 mm, 50×2.1 mm, Agilent Technologies, Santa Clara, CA). The following solvents were used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 2h with primary antibody diluted in Blocking Buffer with 5% Goat Serum (Dako #X090710) (antibodies are dilutions used are listed in Table 2) ...
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bioRxiv - Bioengineering 2024Quote: ... ITO slides were then stained for H&E and imaged using the Cytation 5 (Agilent, Santa Clara, CA) and Epsilon scanner at 3000 dpi to select regions of interest using the open-source program ...
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bioRxiv - Plant Biology 2024Quote: ... The column was an Eclipse Plus C18 column (250 mm x 4.6 mm, 5 µm; Agilent Technologies, USA) with a security guard ultra C18 guard column (4.6 mm x 2 mm ...
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bioRxiv - Microbiology 2024Quote: ... The samples were injected onto a DB-5 column (30 cm by 0.25 mm, 1.0-μm film thickness; Agilent). The injector temperature was set at 280°C ...
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bioRxiv - Immunology 2024Quote: ... BMDMs were plated at a density of 5×104 cells per well into XFe96 cell culture microplates (Agilent) and cultured overnight followed by β-glucan (50 μg/mL ...
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bioRxiv - Synthetic Biology 2024Quote: ... The experiment was conducted using a BioTek Synergy HTX Multi-Mode Microplate reader with Gen 5 software (Agilent), set at a temperature of 37°C ...
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bioRxiv - Immunology 2024Quote: ... Dual luciferase activity was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent, Santa Clara, CA). Normalized luciferase activity was determined by calculating Firefly to Renilla signal ratio.
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bioRxiv - Plant Biology 2024Quote: ... 1uL of sample was injected with a 5:1 split ratio onto a 60m DB-5ms column (Agilent). The initial oven temperature was 120 °C with an oven ramp of 6 °C/min until 300 °C and held for 8 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Automated dilution was performed using 5 μL of solvent A (99.9% water + 0.1% formic acid) with the Bravo liquid handler (Agilent). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... The width of the scratch was measured at each timepoint on the Cytation 5 with Biospa microscope (Agilent).
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... or 1 µM (OMM1) carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP) and 0.5 µM of a rotenone antimycin A mix (Agilent Technologies). Following the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated with the indicated primary antibodies (Reagents) overnight at 4 °C followed by Envision/diaminobenzidine detection (Dako, Glostrup, Denmark) and hematoxylin counterstaining/mounting (Entellan ...
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bioRxiv - Molecular Biology 2020Quote: ... were induced with 2μg/mL doxycycline for 6 hours prior to crosslinking at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Cells were then processed according to the eCLIP protocol for input and immunoprecipitated samples until cDNA was obtained ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Genomics 2021Quote: ... 4 μl of the purified product were amplified in multiple 100 μl reactions using Herculase II Fusion DNA Polymerase (Agilent) following the manufacturer’s specifications with 0.3 μM of the IS5/IS6 primers ...
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... iPSC-derived kidney organoids were cut at a thickness of 4 μm using a cryo-microtome and mounted on FLEX IHC Microscope Slides (DAKO, Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The plasma (50 µL) was diluted with 150 µL of buffer A (1:4 dilutions, as recommended by Agilent Technologies), and then filtered to remove particulates using a 0.45 μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Each RNA sample was processed with fluorescence-labelling to prepare complimentary RNA (cRNA) targets that could be detected by an SBC human ceRNA array V1.0 (4□×□180K, Agilent Technologies). The microarray kit contained 88,371 circRNA probes and 18,853 mRNA probes ...
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bioRxiv - Microbiology 2022Quote: ... esters and 4-vinyl guaiacol concentrations were analysed using headspace solid phase micro-extraction coupled with gas chromatography (Agilent 7890A)- mass spectrometry (Agilent 5975C ...
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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bioRxiv - Developmental Biology 2021Quote: ... Half of the cDNA was amplified for 17 PCR cycles and a 1:4 dilution of the resulting library was assessed by Agilent Bioanalyzer High Sensitivity DNA kit ...
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bioRxiv - Immunology 2020Quote: Extracts and 4-OHE1 standard were analyzed using a liquid chromatography system (LC; 1200 series, Agilent Technologies, Santa Clara, CA) that was equipped with a reversed-phase analytical column (length ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...