Labshake search
Citations for Agilent :
1051 - 1100 of 1245 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Membranes were labelled with primary antibody for 1 hour at room temperature or overnight at 4°C followed by incubation with HRP-conjugated secondary antibodies (Dako). Membranes were developed using the Western Lightning ECL system (Perkin Elmer) ...
-
bioRxiv - Microbiology 2023Quote: ... a mutant version of each of the hsdR1 had a unique BamHI site inserted by site directed mutagenesis of a C at position 1033 of hsdR1 to a G using oligonucleotide directed mutagenesis (5’-CATCAGAGACTTTTTTAGCGGATCCAACCTAAACAAAAAGAC-3’) (Quick-Change Lightning. Agilent). The mutated site is shown in bold and italics and the generated BamHI site is underlined ...
-
bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
-
bioRxiv - Biochemistry 2023Quote: ... Fluorescence emission at 340 nm from the reaction mixture was monitored at 37°C with 15 s intervals using a Biotek Synergy H1 microplate reader equipped with Biotek Gen5 software (Agilent). A linear fit was performed for the measured fluorescence within 10 min reaction time to obtain the rate of decrease in NADH fluorescence ...
-
bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
-
bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were stored at - 80°C until crosslinking (still frozen) with 400 mJ/cm2 UVC using a Stratalinker 2400 (Stratagene).
-
bioRxiv - Microbiology 2023Quote: Log phase cultures of cells grown without inducer were diluted to an OD600 of 0.05 and grown in 96-well plates for 24 h at 30°C with constant shaking on a BioTek Cytation 1 plate reader (Agilent), measuring the absorbance at 600 nm every 30 min for three technical replicates for each strain ...
-
bioRxiv - Cell Biology 2023Quote: ... Kidneys were equilibrated at 37°C for 1 h in a non-CO2 incubator and then assayed on a Seahorse XFe24 Analyzer (Agilent) following a 12-min equilibration period ...
-
bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
-
bioRxiv - Microbiology 2024Quote: ... Extracted metabolites were separated on a Cogent Diamond Hydride Type C column of 1200 liquid chromatography coupled to an Mass 6220 TOF spectrophotometer (Agilent). Ion abundances of metabolites were estimated using Profinder 8.0 and log2 fold changes were analyzed and plotted using Gene Cluster 3.0 and Java Treeview.
-
bioRxiv - Physiology 2024Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:4, diluted in 1% goat serum/PBS, Agilent). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed overnight at 4 °C with rabbit monoclonal anti-PSyn EP1536Y diluted 1:320,000 in DAKO antibody diluent (Agilent #S0809). After three 5-min washes with PBST ...
-
bioRxiv - Plant Biology 2024Quote: ... Root length was measured 24h upon UV-C exposure (300, 600 and 900 J/m2) using the Stratalinker 2400 (Stratagene). The relative root growth was calculated as followed ...
-
bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
-
bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
-
bioRxiv - Biochemistry 2024Quote: ... the fluorescence value was recorded every minute for over 30∼60 min at 37°C by Mx3500P (Agilent, CA, USA). The endpoint detection here was defined as detecting the accumulated fluorescence once at the end of the reaction ...
-
bioRxiv - Biochemistry 2024Quote: ... and subsequently cloned into a pET-SUMO expression vector with an additional C-terminal hexahistidine tag cloned in using QuikChange (Agilent).
-
bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections were progressively rehydrated before antigen retrieval for 30 min at 95°C in 1X Antigen Retrieval Buffer (Dako, 51699). Samples were incubated in perm-block buffer (5% donkey serum ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: All hydrolysis assays were performed in 96-well plates at 30 °C in a BioTek Synergy H1 microplate reader (Agilent). Production of GDP ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
-
bioRxiv - Cell Biology 2022Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 37°C in a non-Co2 incubator and Seahorse XF calibrant solution (Agilent Technologies). After this ...
-
bioRxiv - Genomics 2020Quote: ... HindIII-based Hi-C libraries from our previous study (Wutz et al., 2017) were captured using SureSelect target enrichment system (Agilent Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... followed by three 10 min incubations in 500 μl wash buffer II at 65°C) were performed according to the SureSelect Target enrichment protocol (Agilent Technologies). After the final wash ...
-
bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
-
bioRxiv - Cell Biology 2019Quote: ... Fluorescence intensity (Exitation: 560 nm, Emmission: 590 nm) was recorded at 37°C using the Cary Eclipse fluorescence spectrophotometer (Agilent Technologies). 100 s later when the fluorescence is steady ...
-
bioRxiv - Genetics 2019Quote: ... The PAM sequences in the c(3)G gene were mutated using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent Technology). The bases changed are in bold above ...
-
bioRxiv - Biochemistry 2020Quote: Measurements in both settings were performed in 3 min mix and 3 min measure cycles at 37 °C in six replicates per condition on a Seahorse XFe96 Analyzer (Agilent Technologies). OCR and ECAR were depicted as pmol/min and mpH/min ...
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
-
bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
-
bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...
-
bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
-
bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
-
bioRxiv - Biophysics 2021Quote: ... then was injected to online SEC-SAXS equipped with a temperature-controlled (15 °C) silica-based SEC column (Agilent BioSEC-3)32 ...
-
bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... The samples were loaded on a Superdex 200 10/300 Increase column (Cytiva) at a temperature of 20°C run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 ml/min ...
-
bioRxiv - Biochemistry 2022Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
-
bioRxiv - Molecular Biology 2021Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3µm) operated at 0.6 mL/min and 80°C (Agilent Technologies, Santa Clara, CA) on an HPLC equipped with a diode array detector set for absorbance detection at 214nm and ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The fragment size distribution and concentration of the final PacBio and Dovetail Omni-C library were assessed using a TapeStation (Agilent Technologies) and a Qubit Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
-
bioRxiv - Neuroscience 2021Quote: Paraffin-embedded sections were deparaffinized and autoclaved at 121°C for 20 min in Target Retrieval Solution (Dako Japan Inc., S1700). Frozen sections were briefly washed in PBS and incubated with 0.5 % SDS (v/v ...
-
bioRxiv - Genetics 2022Quote: ... and incubated in the dark overnight at 4 C before running on a Novocyte Quanteon flow cytometer using NovoExpress 1.3.0 software (Agilent, Santa Clara, CA) at a flow rate of 14 uL/minute ...