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Citations for Agilent :
1051 - 1100 of 2353 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: A pET-24B plasmid containing Marseillevirus marseillevirus doublet histones (Hδ-Hγ with a C-terminal 6×His tag and Hβ-Hα) (Valencia-Sanchez et al., 2021) was transformed into Escherichia coli BL2-codon plus(DE3)-RIL competent cells (Agilent Technologies) and cultured at 37°C in 2xYT-Kanamycin medium ...
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bioRxiv - Microbiology 2022Quote: ... coli and used as a template for generation of E2 mutants by a QuikChange Lightning Site Directed Mutagenesis Kit (Agilent Technology) following the supplier’s procedure ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
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bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were rabbit (P044801-2) or mouse (P044701-2) HRP-conjugated (Dako, Agilent), used at 1:10000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were rabbit (P044801-2) or mouse (P044701-2) HRP-conjugated (Dako, Agilent), used at 1:10000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45: 1:1000, 70257 Cell Signaling Technology; CD3: 1:2000 A0452 Dako/Agilent) diluted in TBST+5% goat serum in a humid chamber overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... αIgG (Agilent Cat#A042402-2). Cells were cultured in RPMI-1640 (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... Krt8/18 (DAKO M365201-2), GATA6 (R&D AF1700 ...
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bioRxiv - Neuroscience 2021Quote: ... GFAP (Agilent, Cat# Z033429-2) Stained sections with only secondary antibodies were used as controls ...
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bioRxiv - Neuroscience 2021Quote: ... GFAP (Agilent/Dako, Z033429-2), Lectin (Vector Labs ...
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bioRxiv - Neuroscience 2021Quote: ... GFAP (Agilent/Dako, Z033429-2), Lectin (Vector Labs ...
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bioRxiv - Systems Biology 2020Quote: ... pH 9 (Agilent, S236784-2) for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 9 (Agilent, #S236784-2) at 97 °C for 10 min ...
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bioRxiv - Physiology 2019Quote: ... ACC1/2 (DAKO DENMARK, P0397), phospho-ACC Ser212 (Millipore,03303).HDAC4 (7628 ...
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bioRxiv - Physiology 2021Quote: ... DAB chromogen (Agilent, K346889-2) with hematoxylin counter stain (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... bluing buffer (Dako, CS70230-2) for 90 seconds and finally in Eosin Y (Sigma ...
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bioRxiv - Physiology 2023Quote: ... 2 μM of FCCP (Agilent), and 0.5 μM of rotenone AA (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: ... and 2 mM glutamine (Agilent). Cells were allowed to attach at RT for 15 min and then transferred to a 37°C incubator without CO2 for 40 min ...
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bioRxiv - Cell Biology 2022Quote: ... pH 9.0 (Agilent, S236784-2) for 10 minutes was used for MMP13 and p16 antigen retrieval ...
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bioRxiv - Developmental Biology 2023Quote: ... Insulin (1:2, Dako IR002), Integrin-α6 (1:400 ...
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bioRxiv - Physiology 2023Quote: ... and 2 mM pyruvate (Agilent) and the mitochondria concentration assessed by Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL-2 (Dako #M0887) and anti-β-actin (EMD Millipore #MAB1501) ...
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bioRxiv - Cell Biology 2024Quote: ... Diaminobenzidine (Agilent, Cat#K346811-2) was used as a chromogen and finally ...
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bioRxiv - Neuroscience 2024Quote: ... 2 glutamine (Agilent #103579-100) in XF DMEM medium (Agilent #103575-100)] in atmospheric air at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Dye-swap hybridizations (2+2) were performed on microarray slides (4×44K zebrafish V3, Agilent) using gasket slides and a hybridization chamber and incubated for 17 h at 65°C and 10 rpm in the hybridization oven (Agilent Technologies) ...
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bioRxiv - Biophysics 2021Quote: Wild-type (WT) 3RS-and 4RL-Tau constructs were expressed in BL21 CodonPlus(DE3)-RP Escherichia coli cells (Stratagene, La Jolla, CA) and purified as previously described [17 ...
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bioRxiv - Immunology 2019Quote: Individual subunits (Strep-Tactin and dead streptavidin-SpyCatcher or dead streptavidin and Strep-Tactin-SpyCatcherΔ) were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies # 230280) and refolded from inclusion bodies using a modified version of the protocol previously described by Howarth and Ting43 ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Immunology 2023Quote: Strep-Tactin SpyCatcher and dead streptavidin (monovalent) or Strep-Tactin SpyCatcher and Strep-Tactin (tetravalent) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies #230280) were combined and refolded from inclusion bodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and fixed with precooled methyl alcohol in −20℃ for 30 min followed by H&E staining (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702), sections were processed at RT by isopropanol (MilliporeSigma ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The final library was analyzed using Agilent DNA 1000 chip (Agilent) to estimate the quantity and check size distribution ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor sections with an RNA integrity number of 9.3 (Agilent 1000) and subjected to oligoT magnetic bead enrichment ...
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bioRxiv - Neuroscience 2019Quote: ... Samples purified were then validated using BioAnalyzer (Bioanalyzer DNA 1000, Agilent) and 4 nM of each library pooled using unique indices before sequencing on a MiSeq (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a streptavidin-HRP complex (1:1000, Dako). Immunoreactivity was visualized using DAB (0.4 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... anti-S100 (1/1000) rabbit polyclonal antibody (Z0311, Dako Corp., USA). Fluorescent dye-conjugated secondary antibodies used in appropriate combinations ...
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bioRxiv - Microbiology 2021Quote: ... Quality was evaluated with a Bioanalyzer DNA 1000 chip (Agilent Technologies), and then paired-end sequencing (2 × 125 bp ...
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bioRxiv - Pathology 2021Quote: ... RNA integrity was confirmed using the Agilent Bioanalyzer 1000 (Agilent Technologies).
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bioRxiv - Neuroscience 2022Quote: Primary antibodies used: rabbit anti-GFAP (1:1000, Agilent Dako, USA), rabbit anti-Ezrin (1:300 ...
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bioRxiv - Neuroscience 2022Quote: Primary antibodies used: rabbit anti-GFAP (1:1000, Agilent Dako, USA), rabbit anti-Ezrin (1:300 ...
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bioRxiv - Immunology 2021Quote: ... The library was analyzed using High Sensitivity DNA ScreenTape 1000 (Agilent) for quality and quantification and pulled together for the final library denaturation ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were: rabbit anti-GFAP (1:1000; Dako, Carpinteria, CA); rat anti-GFAP (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... MSH6 (Monoclonal Rabbit Anti-Human; Clone EP49, dilution 1 : 1000 Dako) PMS2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were: rabbit anti-GFAP (1:1000; Dako, Carpinteria, CA); rat anti-GFAP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... and anti-mouse IgG–conjugated HRP (1:1000 dilution; P0447, Dako) was used as the secondary antibody.
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Samples purified were then validated using BioAnalyzer (Bioanalyzer DNA 1000, Agilent) and 4 nM of each library pooled using unique indices before sequencing on a MiSeq (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... Exon skipping was analyzed using the DNA 1000 kit (5067, Agilent) on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara ...