Labshake search
Citations for Agilent :
951 - 1000 of 1111 citations for WesternBright Quantum HRP Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... they were then washed three time in PBS containing 0.1% tween 20 and incubated 1h with a rabbit anti-mouse antibody conjugated with horseradish peroxidase (HRP) (Dako). The activity of HRP was revealed by enhanced chemiluminescence (Perkin-Elmer).
-
bioRxiv - Evolutionary Biology 2021Quote: ... using an anti-GFP antibody (MBL, Code No. 598) and an anti-Rabbit Goat Immuno-globulins/HRP (DAKO, P0160) as 1st and 2nd antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3x with 0.1% PBST and incubated in an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) diluted 1:2000 in 5% milk powder in 0.1% PBST for 2 hours at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... TH immunolabeling with horseradish peroxidase (HRP) - 3,3-diaminobenzidine (DAB) detection and hematoxylin counterstain was performed using an Dako Omnis automated machine (Agilent Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... A goat anti-rabbit HRP polymer (Envision +) was used as secondary antibody and color was developed with DAB + (DAKO / Agilent Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated for 1hr at room temperature with polyclonal HRP-conjugated goat anti-rabbit (1:5000; Dako, Denmark) or anti-mouse (1:5000 ...
-
bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
-
bioRxiv - Pathology 2023Quote: ... Secondary HRP signal was either inactivated by performing two blocking steps of Dako dual endogenous peroxidase block (Dako, S2002) for 10 minutes at room temperature (each) ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the secondary antibody-horseradish peroxidase (HRP) complex from the REAL EnVision detection kit (Dako #K5007) for 1h at RT ...
-
bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with 0.1 % Tween-20 in PBS (PBST) and incubated in an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) diluted 1:2000 for 2 hours at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
-
bioRxiv - Cancer Biology 2023Quote: ... Signals were amplified using the Dako envision+ system-HRP labeled polymer anti-rabbit antibody (Agilent Technologies, Santa Clara, CA). Color development was performed with diaminobenzene (DAB ...
-
bioRxiv - Cell Biology 2024Quote: ... Western blotting analysis was carried out using standard protocols with the antibodies listed in Table_1 and HRP-conjugated secondary antibodies (Agilent). Bands were visualised bu SuperSignal West Fempto ECL substrate (Thermo ...
-
bioRxiv - Pathology 2023Quote: ... then washed in PBS and exposed to the detection kit (EnVision + System-HRP Anti-Rabbit, Dako Denmark A/S) which included 3,3′-diaminobenzidine tetrahydrochloride as chromogen and counterstained with Harris hematoxylin ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the enzymatic activity was measured by detecting cleaved substrate linked to fluoropore using a fluorescence spectrofluorometer (Agilent Varian Cary Eclipse, Hyderabad, India) as described previously [45].
-
bioRxiv - Bioengineering 2019Quote: ... Substrates and inhibitors were injected during measurements at a final concentrations of 3.5 μM 4-(trifluoromethoxy) phenylhydrazone (FCCP; Seahorse Bioscience, Billerica, MA, USA), 1 μM oligomycin ...
-
bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
-
bioRxiv - Cell Biology 2020Quote: ... then probed for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG secondary antibodies 1:500 in PBS (Dako, Agilent Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... sections were incubated with secondary antibody polyclonal rabbit anti-mouse HRP-conjugate (cat.P0260, Dako, Agilent Technologies, Santa Clara, CA, USA) diluted 1:100 in PBS 1× for 1h at room temperature (RT) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... antibody staining was carried out using an anti-RFP antibody for 1h detected with EnVision HRP anti-rabbit secondary (Agilent) followed by incubation with Tyramide-conjugated Opal 570 (PerkinElmer ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
-
bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
-
bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
-
bioRxiv - Immunology 2019Quote: ... Visualization of bound anti-insulin antibody was achieved using HRP-labelled polymer-conjugated goat anti-rabbit IgG (Dako EnVision+ System), followed by counterstain with hematoxylin ...
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako) in a dilution of 1:1500 ...
-
bioRxiv - Immunology 2021Quote: ... rabbit anti-VWF (A0082, 1:1000) and rabbit anti-VWF-HRP (P0226, 1:8000) used for sandwich ELISA were purchased from Dako, sheep anti-VWF (ab11713 ...
-
bioRxiv - Immunology 2021Quote: ... paraffin-embedded tissues were cut at 5uM and stained using Envision+System-HRP (DAB) chemistry and AutostainerPlus hardware (both Dako) by the City of Hope Pathology Core shared resource ...
-
bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
-
bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody at a dilution of 1:7000 (Dako) or 1:2000 (Pierce ...
-
bioRxiv - Microbiology 2021Quote: ... and then with streptavidin-HRP complex followed by 3,3-diaminobenzidine or 3-amino-9-ethylcarbazole detection (LSAB kit, Dako France). Sections were then counterstained with hematoxylin ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS + 0.1% Tween 20 and 5% dried skimmed milk powder and probed with relevant primary antibodies followed by horseradish peroxidase (HRP)-conjugated polyclonal goat anti-mouse and polyclonal goat anti-rabbit secondary antibodies (Dako). Membranes were treated with EZ-ECL (Geneflow ...
-
bioRxiv - Microbiology 2020Quote: ... The blocked membrane was then probed with anti-HA (1:1000 dilution, CellSignalling) and rabbit anti-mouse P260 IgG conjugated with HRP (1:1000 dilution, DAKO). The antibody-stained membrane was then soaked with SuperSignal West Pico PLUS substrate (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides were then washed for 3 min in TBST and incubated for 30 min with HRP (Horse Raddish Peroxidase) conjugated anti-rabbit (Ref: K4003, Dako) or anti-mouse (Ref ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved on 10–16% gradient SDS-PAGE under reducing conditions followed by immunoblotting for PMCA (primary antibody: 5F10 anti-pan-PMCA, Abcam, ab2825, secondary antibody: anti-mouse-HRP, Dako) and aSN (primary antibody ...
-
bioRxiv - Pathology 2022Quote: ... appropriate HRP-coupled secondary antibodies (rabbit anti-mouse, Cat. No. P0260, and goat anti-rabbit, Cat. No. P0448, from DAKO and rabbit anti-goat ...
-
bioRxiv - Molecular Biology 2022Quote: ... sections were incubated for 30 minutes in DAKO Envision-Plus System HRP Labelled Polymer Anti-Mouse (DAKO, Catalog No. K4001). Peroxidase labeling was visualized with the Liquid DAB+ Substrate Chromogen System (DAKO ...
-
bioRxiv - Molecular Biology 2022Quote: ... Integrin α11 expression levels were detected by rabbit polyclonal antibodies to human or mouse integrin α11 (provided by Donald Gullberg, Department of Biomedicine, University of Bergen, Norway) and the polyclonal anti-rabbit immunoglobulins/HRP (Dako). Images were analyzed using the gel analyzing tool by ImageJ ...
-
bioRxiv - Physiology 2022Quote: ... Membranes were washed with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: swine anti-rabbit Ig HRP-conjugated (1:3000, catalog no. P0399, Dako) or goat anti-mouse Ig HRP-conjugated (1:5000 ...
-
bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... For signal detection the MACH 3 Mouse HRP Polymer Detection system was employed according to manufacturer’s protocol using DAB+ (Fa.Agilent Technologies, K3468). Slides were counter-stained with Hematoxylin Gill’s Formula (Fa.Vector ...
-
bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 nucleocapsid (EY-2A, a kind gift from Prof Alain Townsend) primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). Chemiluminescence substrate (West Dura ...
-
bioRxiv - Immunology 2022Quote: ... The membranes were washed and incubated with secondary swine anti-rabbit HRP conjugated antibody (Agilent DAKO, Santa Clara, CA, P0399) in 1% milk in PBST for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were then washed twice with wash buffer before a 20-minute incubation with Envision FLEX HRP Polymer (GV823, Agilent DAKO) for HRP binding ...
-
bioRxiv - Molecular Biology 2022Quote: ... Sections were immunostained with the primary antibody for 60 min and subsequently incubated with Dako EnVision HRP secondary antibody (Dako) for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...