Labshake search
Citations for Agilent :
951 - 1000 of 1459 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
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bioRxiv - Developmental Biology 2021Quote: ... Half of the cDNA was amplified for 17 PCR cycles and a 1:4 dilution of the resulting library was assessed by Agilent Bioanalyzer High Sensitivity DNA kit ...
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bioRxiv - Immunology 2020Quote: Extracts and 4-OHE1 standard were analyzed using a liquid chromatography system (LC; 1200 series, Agilent Technologies, Santa Clara, CA) that was equipped with a reversed-phase analytical column (length ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Microbiology 2023Quote: Pooled gradient fractions were diluted 1:2 with ice-cold PBS and tumbled overnight at 4°C with 50 µl Strataclean resin (Agilent). Beads were pelleted at 600 RCF for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The adapter-modified DNA fragments were enriched by 4 cycles of PCR using SureSelect forward and SureSelect ILM Pre-Capture Indexing reverse (Agilent) primers ...
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bioRxiv - Biochemistry 2024Quote: ... and 3D z-stacks were taken of each replicate well monitored every 4 hours for 48 hours using a Cytation C10 imager (Agilent) with confocal 546 nm excitation laser ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed overnight at 4 °C with rabbit monoclonal anti-PSyn EP1536Y diluted 1:320,000 in DAKO antibody diluent (Agilent #S0809). After three 5-min washes with PBST ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Microbiology 2023Quote: ... Growth curves of OD578 were monitored every hour after 1 min of linear shaking under anaerobic conditions using an Epoch2 microplate reader coupled with a Biostack 4 microplate stacker (both Agilent) housed in a custom-made incubator (EMBL workshop24) ...
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bioRxiv - Genetics 2023Quote: ... Membranes were labelled with primary antibody for 1 hour at room temperature or overnight at 4°C followed by incubation with HRP-conjugated secondary antibodies (Dako). Membranes were developed using the Western Lightning ECL system (Perkin Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... This system makes it possible to culture many bacterial strains in parallel in a replicated manner using many 96-well plates which are handled with a stacker (BioStack 4, Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Cells were imaged using a 60x Plan Apo 1.4 NA objective on a Nikon Ti2-E microscope with a Yokogawa X1 spinning disk confocal system, MLC400B 4-line (405nm, 488nm, 561nm, and 647nm) dual-fiber laser combiner (Agilent), Prime 95B back-thinned sCMOS camera (Teledyne Photometrics) ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Microbiology 2022Quote: ... One hundred μL of the extract was mixed with 10μL of 4-chlorobenzotrichloride as injection standard and analyzed by GC (HP 7890 Series GC, Agilent, USA) with a 20 m 0.18mm (18μm film thickness ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with Alamar Blue for 3-4 hours and then imaged with a Synergy Neo plate reader using excitation: 550 nm and emission: 590 nm (Agilent). The average plate background (media only with 0.1% DMSO ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... and diluted to 3.5% nitric acid by adding 3800 μL milliQ water after which all test tubes containing samples were placed into an automatic sampler (SPS 4 Autosampler, Agilent) integrated with the MP-AES (model 4210 ...
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bioRxiv - Plant Biology 2024Quote: ... Temperature-dependent phase transition was detected by measuring the optical scattering at 800 nm while increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface ...
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bioRxiv - Physiology 2024Quote: ... RNA concentration and integrity were evaluated by a fluorimetric assay (Qubit 4, ThermoScientific, USA) and capillary electrophoresis (TapeStation, Agilent, Germany), respectively ...
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bioRxiv - Microbiology 2024Quote: ... Nine images were taken every 4 hours in each well and the number of cells by images were determined using BioTek Gen5 Software (Agilent).
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bioRxiv - Immunology 2024Quote: 0.4 ug of biotinylated SL9 (SLYNTAVTL) or IV9 (ILKEPVHGV) peptides (NIH Tetramer Core Facility) were conjugated to PE-streptavidin (Agilent), APC-streptavidin (Agilent ...
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bioRxiv - Biochemistry 2024Quote: ... mutations were inserted into the WT isolated (residues 193 – 261) RRM2 domain in a modified pGEX-6p1 vector (4) using the QuickChange method (Stratagene) and verified through Sanger Sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and quantity of the enriched libraries were evaluated using Qubit® 4 Fluorometer and 4200 TapeStation System (Agilent). Sequencing was performed on an Illumina NovaSeq6000 machine with single-end 100 bp reads for NPM1 and H3K9me2 according to standard protocols.
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were incubated for 2 h at 4℃ in a guinea pig anti-insulin antibody (1:200, A0564, Dako), a rabbit anti-somatostatin antibody (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were automatically taken every 4 hours over 72 hours in a BioTek BioSpa Automated Incubator (Agilent, Santa Clara, CA). Cell proliferation was quantified using Aligent’s Gen5 image analysis tools.
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated for each aaRS activity ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated as the GFP synthesis rate ...
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Biochemistry 2022Quote: ... All libraries at this step yielded >300,000 colonies implying a 100x coverage (assuming 1/3 of the variants were perfect based on our previous analysis of Agilent OLS based libraries). Each sublibrary was combined at an equimolar ratio to make a complete library with all intended mutations included.
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Plant Biology 2024Quote: ... For separation of all metabolites, we used a Zorbax RRHD Eclipse XDB-C18 column (50 x 3 mm, 1.8 µm) (Agilent, Santa Clara, CA, USA) with a 1290 Infinity II inline filter (0.3 µm ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...