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Citations for Agilent :
51 - 100 of 1131 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 17 500 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10 μm particle size ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were mouse anti-Cytokeratin 7 (DAKO - M7018, 1:400), Rabbit anti-Cytokeratin 20 (Abcam - ab76126 ...
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bioRxiv - Neuroscience 2021Quote: ... Images were acquired on a 7 Tesla MRI scanner (Agilent Inc.) (23 ...
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bioRxiv - Immunology 2023Quote: ... or R-phycoerythrin-cyanine-7 (PE-Cy7) fluorochromes (Prozyme, Thermo-Fisher).
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bioRxiv - Immunology 2024Quote: ... Samples with an RNA integrity number (RIN) below 7 (BioAnalyzer, Agilent) did not proceed to sequencing.
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bioRxiv - Biochemistry 2024Quote: ... Only high-quality RNA (RNA integrity number > 7, Agilent 2100 Bioanalyzer) was used for microarray analysis ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight at 4°C with 10 µg/ml polyclonal rabbit anti-human/mouse fibrin(ogen) (Dako), 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26 ...
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bioRxiv - Biochemistry 2021Quote: NIH 3T3 cells were transiently transfected with pFA2-cJunWT (PathDetect c-Jun trans-Reporting System from Agilent Technologies), or the indicated pFA2-cJun mutants or pFC2-dbd (empty vector) ...
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bioRxiv - Immunology 2023Quote: ... A 13 kb genomic region harboring the ATG start codon was subcloned into the pBlueScript II vector (Stratagene). The DNA fragment harboring partial exon2 sequences and Venus cDNA sequences were generated by overlapping PCR technique and was used to construct a targeting vector ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed 2 x 15 min in PBST and 2 x 15 min in PBS and coverslipped with Fluorescence Mounting Medium (DAKO #S3023).
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Genomics 2020Quote: ... Hybridisations were carried out over 17 hours at 65° C at 10rpm rotation and washed following manufacturer’s instructions (Agilent). Datasets are available at ArrayExpress ...
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Immunology 2021Quote: ... the following mutations were introduced into the BG505 SOSIPv5.2 construct(17) using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent): P240T ...
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bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Immunology 2021Quote: A multi-channel 7 Tesla MRI scanner (Agilent Inc., Palo Alto, CA) was used to image brains in skulls ...
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bioRxiv - Neuroscience 2023Quote: ... At day 7 of differentiation astrocytes were switched to XF media (Agilent) and then assessed using the previously described Mitochondrial Stress Test Protocol [24] ...
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bioRxiv - Genetics 2023Quote: ... resulting from Cas12a based trans-cleavage were measured every 2.5 min using a BioTek Synergy 2 microplate reader (Agilent). In a diagnostic specificity assay using field samples ...
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bioRxiv - Microbiology 2021Quote: ... MDA-amplified environmental DNA isolated from the Lower Culex Basin at Yellowstone National Park (USA)17 was then amplified with Herculase polymerase (Agilent). Amplification of control and gap regions was then performed following the parameters shown in Suppl ...
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bioRxiv - Plant Biology 2021Quote: ... Final libraries were amplified with 17 cycles of PCR and assessed on the Bioanalyzer with the High Sensitivity DNA kit (Agilent). All root tissue libraries that were sequenced comprised two biological replicates.
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bioRxiv - Microbiology 2020Quote: ... The first reaction resulted in a plasmid containing 2,000 bp-long homologous arms (amplified from the genomic DNA of V. dahliae isolate Ls.17 using the Herculase II Fusion DNA polymerase; Agilent) flanking the neo cassette (conferring resistance to geneticin ...
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bioRxiv - Neuroscience 2021Quote: ... micro-capillary column (450 mm OD x 320 mm ID x 17 cm) using an Agilent 1200 Series HPLC (Agilent). Peptides were separated using a gradient from 100–60% solvent B (A = 0.1% TFA ...
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bioRxiv - Biochemistry 2021Quote: ... or PDK1SKD-PIF S241A (4 mg/ml) was injected onto a Superdex 200 10/300 column (Cytiva) operated by a 1260 Infinity HPLC (Agilent Technologies). Light scattering of a 690 nm laser was detected by a MiniDawn Treos (Wyatt ...
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bioRxiv - Neuroscience 2024Quote: ... sections were post-fixed with 4% formaldehyde for 10 minutes and histologically stained with Dako Mayers hematoxylin (Agilent, cat. no. S3309) (4 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... B’β or GFP and empty vector control or shRNA-expressing plasmid were combined with the PathDetect Elk1 trans-reporting system (Agilent) and a SV40-pNL1.1 ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Peaks were extracted using MasterHands, automatic integration software (Keio University, Tsuruoka, Yamagata, Japan)13 and MassHunter Quantitative Analysis B.04.00 (Agilent Technologies) to obtain peak information including m/z ...
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bioRxiv - Immunology 2023Quote: ... in the α3 domain of HLA-A*02:01 described to abrogate binding of CD8 (13) were changed by site-directed mutagenesis (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed by NovoCyte 3000 flow cytometer equipped with three lasers and 13 fluorescence detectors (Agilent, Santa Clara, CA). GFP and Alexa Flour 647 fluorophores were excited by the 488 and 640 nm lasers ...
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bioRxiv - Molecular Biology 2021Quote: ... was generated from a 7-kb genomic clone from Lambda FIX Library (Stratagene) (Supplemental Fig ...
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bioRxiv - Neuroscience 2022Quote: ... Postmortem data were then acquired on an 7 T small animal scanner (Agilent) fitted with a 40 G/cm gradient coil (Agilent ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality (RIN > 7) was confirmed using an RNA 6000 Nano Kit (Agilent). Spatial gene expression slides were processed following manufacturer instructions (Visium Spatial Gene Expression Reagent Kits User Guide ...
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bioRxiv - Immunology 2023Quote: ... or cyan-7 conjugated R-phycoerythrin (PECy7) fluorochrome-conjugated streptavidin (Prozyme, Thermo-Fisher).
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bioRxiv - Developmental Biology 2024Quote: ... and 7 using the Agilent SureSelect Kit (Agilent Technologies, Santa Clara, CA, USA). Whole-genome sequencing was carried out for the proband of Pedigree 5 using the DNBSEQ-T7 platform (Huada ...
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bioRxiv - Microbiology 2020Quote: Fatty acid methyl esters were obtained as previously described [17] and separated by using a gas chromatograph (model 6890 N; Agilent Technologies). Peaks were automatically computed and assigned using the Microbial Identification software package (MIDI) ...
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bioRxiv - Biochemistry 2023Quote: ... was expressed and purified following the method published previously.17 The L230G STEAP1 mutation was introduced by the QuikChange method (Stratagene, CA) using the primers:
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bioRxiv - Biochemistry 2024Quote: ... was added to reach a final reaction volume of 100 µL and substrate hydrolysis was measured at OD405nm every 15 s for 15 min at 30°C using the Synergy H1 microplate reader (Agilent, Santa Clara, CA, USA). The observed substrate hydrolysis rate in the absence of ixochymostatin was considered 100% and compared with the remaining enzymatic activity in the presence of the inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... Trans) following the manufacturer’s instructions and checked for RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNAClean XP Kit (Cat#A63987 ...
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bioRxiv - Cell Biology 2021Quote: ... except that in this case the cDNA was synthesized from 1 µg (testis) or 13-20 ng (spermatozoa) of RNA using the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent 200820) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Trio WES of 13 patients and their parents was performed using the SureSelect Human All Exon V5+UTR kit (Agilent technologies). In patient 4 ...
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bioRxiv - Microbiology 2023Quote: ... with 13 cycles of 30 s on followed by 30 s off and quantified using an Agilent D1000 ScreenTape (Agilent Technologies); DNA fragment size after this step averaged 240 bp.
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were then PCR-amplified with 13 cycles and the final library quality was assessed using a TapeStation System (Agilent Technologies), followed by a second size selection ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed twice with wash buffer and data were acquired on a NovoCyte 3000 flow cytometer equipped with three lasers (405nm, 488nm, and 640 nm) and 13 fluorescence detectors using the NovoExpress (v. 1.6.2) software (Agilent, Santa Clara, CA). Data analysis was carried out using FlowJo (v ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA concentrations were between 17 and 66 ng/uL in volumes of 30 or 40 uL H2O with integrity numbers (RIN, Bioanalyzer, Agilent Technologies, USA) between 6.3 and 7.9 (Table S1) ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... 200 μL was pipetted into a polypropylene test tube (17 mm diameter and 130 mm height, Agilent Technologies, Santa Clara, CA, USA) and diluted to 3.5% nitric acid by adding 3800 μL milliQ water after which all test tubes containing samples were placed into an automatic sampler (SPS 4 Autosampler ...
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bioRxiv - Neuroscience 2021Quote: A 7 T horizontal small-bore magnet and (Agilent Technologies Inc, Santa Clara, USA) and a quadrature volume radiofrequency coil (39 mm internal diameter ...
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bioRxiv - Cell Biology 2020Quote: ... Samples with RIN values above 7 in the Bioanalyzer RNA 6000 Nano assay (Agilent) were used for the synthesis of complementary DNA and RT controls using a reverse transcription TATAA GrandScript cDNA Synthesis Kit (TATAA Biocenter) ...
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bioRxiv - Genomics 2024Quote: ... the integrity (RIN > 7) and concentration (ng/µl) were accessed using Bioanalyzer 2100 (Agilent). At last ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... 7-µm-thick paraffin sections were deparaffinized and autoclaved in target retrieval solution (DAKO) for 10 min at 121°C ...