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Citations for Agilent :
51 - 100 of 2353 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: Human BRAF kinase domain (residues 448-723 with a tobacco etch virus (TEV)-protease-cleavable N-terminal 6x-His tag in pProEX containing 16 mutations to improve solubility38 was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Microbiology 2020Quote: His-tagged VP35 IID (VP35 aa 215-340) and GST-NP (aa 412-500) or shorter variants were expressed in the BL21 CodonPlus strain (Agilent Technologies). Cells were lysed in Buffer (0.15 ...
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bioRxiv - Cell Biology 2021Quote: ... A Ddyo7-mCherry expression plasmid was generated by first TA cloning a PCR product (myo42 to myi185+2) encompassing the myoi 3’ region of the gene (aa 475 - end) minus the stop codon using StrataClone (Agilent) (pDTi289+2) ...
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bioRxiv - Systems Biology 2019Quote: ... and Spike Kit (Agilent, UK). cDNAs were hybridized to Mouse miRNA Microarray ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used were as follows: E-cadherin antibody (M361201-2, Dako), anti-Filaggrin (sc-66192 ...
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bioRxiv - Microbiology 2022Quote: ... Iron in each sample was determined by atomic absorbance spectroscopy (AAS) using a flame 55 AA Absorption Spectrometer (Agilent, Santa Clara, CA, USA). Analytical grade standards for iron (Thermo Fischer Scientific ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... anti-mouse (Dako P044701-2; 1:1000) Alexa Fluor 488 or anti-rabbit (#1706515 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... anti-GFAP rabbit (Agilent Cat# 033401-2; 1:1000 ICC, 1:1000 IHC), anti-GFAP chicken (Encorbio Cat# CPCA-GFAP ...
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bioRxiv - Developmental Biology 2022Quote: ... and anti-SMA (M085129-2, 1:1000, Agilent). All commercial antibodies are validated by vendors ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-Gfap (1:1000, Agilent #Z033401-2); rabbit anti-alpha-Sma (1:200 ...
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bioRxiv - Genomics 2023Quote: ... and GFAP (1:1000, Rabbit, DAKO, Z033401-2). Following primary antibody incubation sections were washed 4x2 minutes with TBST and stained with species-appropriate secondary antibody conjugated to a Horseradish Peroxidase (HRP ...
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bioRxiv - Neuroscience 2024Quote: ... For GFAP (1:1000, Agilent Technologies, Z033429-2), Iba1 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) were chemically transformed with 1:4 diluted Gibson assembly products and plated onto ampicillin-agarose plates ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Agilent) were used for cloning other plasmids ...
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bioRxiv - Genomics 2019Quote: ... coli (Agilent). After cultures reached an OD600 nm of 0.5 ...
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bioRxiv - Immunology 2021Quote: ... coli (Agilent) were transformed with pET-6xHis-SUMO bacterial expression vectors for full-length ORF9 or C-terminally truncated ORF9 1–244 (ORF9-N) ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) as previously described (57) ...
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bioRxiv - Microbiology 2021Quote: ... coli (Agilent), grown as starter cultures in MDG media (0.5% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Stratagene) using glutathione (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) grown in high salt Luria broth (Sigma ...
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bioRxiv - Immunology 2020Quote: ... coli (Agilent), and purified with glutathione sepharose (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... coli (Agilent). Dilutions of 1:1000 and 1:10,000 were plated on LB agar plates supplemented with 100 μg/mL ampicillin to estimate the number of unique transformants in each library ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli (Agilent) and colonies were grown on MDG agar plates (1.5% agar ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (89 ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent). Bacterial cell pellets were lysed using a cell disruptor in the presence of 2.0 mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with pET15b p97 ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with the UBXD1 expression vectors and used for large-scale expression ...
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bioRxiv - Genomics 2023Quote: ... coli (Stratagene) for protein production using Isopropyl β-d-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent). Uniformly labeled (U-13C,15N ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Agilent) were transformed with a vector containing sequences encoding for GELYTACs or nanobody only controls with pelB signal sequence for localization to the periplasm for disulfide bond formation ...
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bioRxiv - Microbiology 2024Quote: ... coli (Agilent), colonies were grown on 1.5% agar MDG plates (2 mM MgSO4 ...
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bioRxiv - Biophysics 2023Quote: ... and E-cadherin (E-cadherin Monoclonal Mouse clone NCH-38, DAKO), in blocking solution (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... pCMV-Tag 2A (Agilent) was used for N-terminal flag tagging using EcoRI and HindIII flanking sites ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Stu2KR/AA mutations were introduced into the Stu2WT background using QuikChange (Stratagene) mutagenesis ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG (Agilent, 1:800 dilution), rabbit anti-V5 (GenScript ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... coli (Agilent #200314). Colonies were miniprepped and Sanger sequenced using a set of primers to ensure proper assembly and in-frame V regions (Supplementary Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Agilent). Cells were grown to optical density at 600nm (OD600 ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent technologies). Small-scale expression was done in 5 ml of auto-inducible terrific broth media (ForMedium ...
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bioRxiv - Physiology 2019Quote: ... coli (Agilent Technologies). 30 ml bacterial starter cultures were grown at 37° C overnight and then added to 1.5 liters of LB media at 37° C ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (Stratagene) with an N-terminal TEV cleavable GST tag ...