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Citations for Agilent :
51 - 100 of 2581 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and Hematoxylin and Eosin (H&E) (Agilent Technologies, #CS11830-2) staining was performed using the DAKO CoverStainer ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Biochemistry 2021Quote: ... Fusion proteins were expressed and purified from BL21 Codon RIL (Stratagene). Expression was induced by adding 0.15 mM IPTG for 4,5 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Fusion protein was expressed in E.coli BL21(DE3) CodonPlus-RIL (Stratagene) in Super Broth Auto Induction Media (Formedium ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... 2 µL Herculase II Fusion DNA polymerase (Agilent Technologies, Cat# 600677), 20 µL 5X Herculase buffer ...
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bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
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bioRxiv - Plant Biology 2021Quote: ... MBP and MBP-COP1 fusion protein were induced in BL21-CodonPlus (Stratagene) E ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: Human BRAF kinase domain (residues 448-723 with a tobacco etch virus (TEV)-protease-cleavable N-terminal 6x-His tag in pProEX containing 16 mutations to improve solubility38 was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were expressed using Escherichia coli BL21 gold strain (Agilent Technology Inc.) cultured in Terrific Broth (TB ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was performed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Agilent). Bacterial cell cultures were grown in LB at 37°C to an OD600 of 0.6 ...
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bioRxiv - Biochemistry 2023Quote: Protein complexes were overproduced in BL21-CodonPlus (DE3) Escherichia coli cells (Agilent) grown in high salt LB Broth (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The RNase E recognition site mutant fusions cfa’-’lacZ-LongG1 and G2 were made by mutating pCB1 using QuikChange mutagenesis (Agilent Technologies) and oligos that restore the G101C mutation to the WT and add the additional desired mutations ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used were as follows: E-cadherin antibody (M361201-2, Dako), anti-Filaggrin (sc-66192 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Cell Biology 2022Quote: ... The expression of the GST-GAP fusion protein in E.coli strain BL-21-RILP (Stratagene) and its purification using Glutathione Sepharose 4B were performed as described previously (Jung et al. ...
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bioRxiv - Biochemistry 2021Quote: ... All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene) using an autoinduction protocol (Studier ...
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bioRxiv - Biochemistry 2021Quote: The KlNmd4 protein was expressed in Escherischia coli BL21(DE3) Gold strain (Agilent technologies) using pMG897 plasmid and 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) were chemically transformed with 1:4 diluted Gibson assembly products and plated onto ampicillin-agarose plates ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Agilent) were used for cloning other plasmids ...
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bioRxiv - Genomics 2019Quote: ... coli (Agilent). After cultures reached an OD600 nm of 0.5 ...
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bioRxiv - Immunology 2021Quote: ... coli (Agilent) were transformed with pET-6xHis-SUMO bacterial expression vectors for full-length ORF9 or C-terminally truncated ORF9 1–244 (ORF9-N) ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) as previously described (57) ...
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bioRxiv - Microbiology 2021Quote: ... coli (Agilent), grown as starter cultures in MDG media (0.5% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Stratagene) using glutathione (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) grown in high salt Luria broth (Sigma ...
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bioRxiv - Immunology 2020Quote: ... coli (Agilent), and purified with glutathione sepharose (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... coli (Agilent). Dilutions of 1:1000 and 1:10,000 were plated on LB agar plates supplemented with 100 μg/mL ampicillin to estimate the number of unique transformants in each library ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli (Agilent) and colonies were grown on MDG agar plates (1.5% agar ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (89 ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent). Bacterial cell pellets were lysed using a cell disruptor in the presence of 2.0 mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with pET15b p97 ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with the UBXD1 expression vectors and used for large-scale expression ...
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bioRxiv - Genomics 2023Quote: ... coli (Stratagene) for protein production using Isopropyl β-d-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent). Uniformly labeled (U-13C,15N ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Agilent) were transformed with a vector containing sequences encoding for GELYTACs or nanobody only controls with pelB signal sequence for localization to the periplasm for disulfide bond formation ...
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bioRxiv - Microbiology 2024Quote: ... coli (Agilent), colonies were grown on 1.5% agar MDG plates (2 mM MgSO4 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...