Labshake search
Citations for Agilent :
51 - 88 of 88 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... which was labelled with alpha-32P-ATP using the Prime-It II Random Primer Labelling Kit (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Mitochondrial stress test or ATP rate assay protocols were executed using a Seahorse XFe24 analyzer (Agilent Technologies), which includes the injection of electron transport chain inhibitors ...
-
bioRxiv - Biochemistry 2024Quote: ... The unhydrolyzed GTP that was converted to ATP was recorded on BioTek Synergy HT (Agilent, Winooski, VT).
-
bioRxiv - Cell Biology 2023Quote: ... Seahorse XF Cell Mitostress test and Real-Time ATP Rate Assays (Agilent Technologies, Santa Clara, CA, USA) were used to evaluate mitochondrial function ...
-
bioRxiv - Molecular Biology 2023Quote: ... The XF Real-Time ATP Rate Assay Kit (Cat. No. 103592-100, Agilent Technologies, Cedar Creek, TX) was performed according to the manufacturer’s protocol using the Seahorse XF96 Analyzer (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
-
bioRxiv - Developmental Biology 2022Quote: ... the sections were washed twice in TTBS buffer before incubation in a diluted solution of Fluorescein (FITC) conjugated F (ab’)₂ fragment goat anti-rabbit IgG (Dako) or Alexa Fluor 647 conjugated F(ab)2 fragment swine anti-rabbit secondary antibody (Molecular Probes) ...
-
bioRxiv - Physiology 2021Quote: ... The contribution of respiration and glycolysis to ATP production were calculated using the Seahorse Analytics web application (Agilent). Measurements were normalized to protein content as determined by BCA assay (Thermo Fisher Scientific).
-
bioRxiv - Physiology 2024Quote: ... ATP production was assessed by measurement of proton extrusion rate (PER) on a Seahorse XFe96 instrument (Agilent Technologies). The PER was measured every 13 minutes for a 3-minute interval before sequential challenge with 1 ...
-
bioRxiv - Cancer Biology 2024Quote: The assessment of mitochondrial activity and ATP production was conducted using a Seahorse XF analyzer (Agilent, California, USA) along with the mitochondrial stress kit (Agilent ...
-
bioRxiv - Neuroscience 2022Quote: ... Mitochondrial respiration and ATP production were evaluated using the Seahorse XFe24 Extracellular Flux Analyzer (Agilent, Santa Clara, CA) by comparing the oxygen consumption rate (OCR ...
-
bioRxiv - Developmental Biology 2023Quote: ... Total ATP generation as well as mitochondrial and glycolytic contributions were determined using the Wave Desktop Application (Agilent) for the conducted assay.
-
bioRxiv - Cancer Biology 2023Quote: Mitochondria respiration and real-time ATP production was assessed by Seahorse XF Cell Mito Stress Test Kit (Agilent) and Seahorse XF Real-Time ATP Rate Assay Kit (Agilent) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The ATP Assay and Mito Stress Assay programs were run on a Seahorse XFe96 (Agilent, Santa Clara, CA) as we previously described (30).
-
bioRxiv - Cell Biology 2021Quote: ... Extracellular flux assay to measure mitochondrial respiration and ATP production was carried out as recommended by manufacturer’s protocol (Agilent). SRB assay (35 ...
-
bioRxiv - Neuroscience 2022Quote: Mitochondrial respiration-derived and glycolysis-derived ATP production rates were determined in primary astrocytes by measuring OCR and ECAR under basal conditions and in response to 1.5 μg/mL oligomycin and 0.5μM/0.5μM rotenone/antimycin A (all XFp Real-Time ATP Rate Assay Kit, Agilent Technologies, 103591-100) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: Extracellular flux analysis (Seahorse) was conducted using the Seahorse XF Real-Time ATP rate assay kit (Agilent, 103592-100). The day prior to the assay ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were next washed three times with PBS and incubated with a secondary fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200) in 1% BSA for 1 h at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Glycolytic and mitochondrial ATP production rates were calculated according to the “Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology” White Paper (Agilent).
-
bioRxiv - Cancer Biology 2022Quote: Real-time changes in metabolism were tested using a Seahorse XFe96 Analyzer in conjunction with the Seahorse XF Glycolysis Stress Test Assay Kit and the Seahorse XF Real-time ATP Assay Rate Kit (Agilent). Manufacturer instructions were followed to perform each of the assays ...
-
bioRxiv - Cancer Biology 2020Quote: ... the cell media was replaced with the assay media and ran using the Seahorse XF Cell Mito Stress Test and ATP Rate Assay on a Seahorse XFe96/XF96 analyzer (Agilent). Hoechst 33342 staining (ThermoFisher ...
-
bioRxiv - Neuroscience 2022Quote: Mitochondrial and glycolytic function was analyzed with the Seahorse Cell Mito Stress Test and Seahorse Real-Time ATP Rate Assay on a Seahorse XFe24 Extracellular Flux Analyzer (Agilent). Neurons were treated on day 11 of culture with 500 μL of fresh neuronal media plus 500 μL of astrocytic conditioned media of varying treatment ...
-
bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
-
bioRxiv - Cancer Biology 2022Quote: Percent ATP produced by mitochondria and the level of compensatory glycolysis were measured using a Seahorse XF Real-Time ATP Rate Assay Kit and XFe96 Analyzers (Agilent) according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... On day 2 the XF Real-Time ATP Rate Assay Kit (Cat. No. 103592-100, Agilent Technologies, Cedar Creek, TX) was run following the manufacturer’s protocol using the Seahorse XF96 Analyzer as described above.
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
-
bioRxiv - Microbiology 2024Quote: Seahorse analyses of the energy metabolism in activated CD4 T cells were performed using the ATP rate assay (Seahorse Biosciences, Agilent) following manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... and bioenergetic profiles were measured with Real-Time ATP Rate Assay and Long Chain Fatty Acid (LCFA) Oxidation Stress Assay (Agilent). Concentrations of the used inhibitors were ...
-
bioRxiv - Genomics 2021Quote: ... Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent, 300385) using PCR generated DNA template produced from gDNA isolated from a wild type S.pombe strain (YP71 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Real-Time ATP production rate were measured using the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent Technologies, Santa Clara, CA). To measure glucose-dependent mitochondrial respiration ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Real-Time ATP production rate were measured using the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent Technologies, Santa Clara, CA). To measure glucose-dependent mitochondrial respiration ...
-
bioRxiv - Cell Biology 2023Quote: Mitochondrial oxygen consumption linked to ATP production was measured using a Seahorse Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA, EUA) as described previously (Vilas-Boas et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... The ATP production from mitochondrial respiration and glycolytic respiration in response to Oligomycin and Rotenone/Antimycin A was measured using the ATP production assay kit (Agilent, USA). The oxygen consumption rate (OCR ...
-
bioRxiv - Cancer Biology 2024Quote: ... was used to measure oxygen consumption and extracellular acidification rates as described for the Real-time ATP rate assay (Agilent Technologies). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: The rates of ATP generation from glycolysis and mitochondria were measured via the Agilent Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, CA) as described 80 ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then washed 1X with 200 mL warm ATP Assay Medium prepared per protocol (Agilent XF DMEM Medium pH 7.4 (103757-100); 10 mM XF Glucose ...
-
bioRxiv - Immunology 2023Quote: Kinetic analysis of cellular glycolysis and oxygen consumption and the rate of ATP production was accomplished through the use of the Extracellular Flux Analyzer XFe96 (Agilent/Seahorse, CA, USA). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... Basal ATP production rates were calculated using the Seahorse XF Real-Time ATP Rate Assay Kit Report Generator (Wave Desktop Software, Agilent Technologies, Cedar Creek, TX). Briefly ...