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Citations for Agilent :
51 - 88 of 88 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CF (R&D/BT10500-050) was incubated at a 4:1 molar ratio with either streptavidin-PE (Prozyme PJRS25) or streptavidin-APC (Prozyme PJ27S ...
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bioRxiv - Plant Biology 2020Quote: ... The QuEChERS extraction pouch for sample extraction and the d-SPE tube for sample purification were purchased from Agilent Technologies (USA) ...
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bioRxiv - Plant Biology 2022Quote: ... Site-directed mutagenesis of pH4::H4 in pENTR/D using QuikChange II XL (Agilent Technologies, Santa Clara, CA, USA) was first performed to create plasmids with 10 silent mutations in the H4 coding sequence ...
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bioRxiv - Immunology 2024Quote: ... 400,000 T cells/well were plated onto Poly-D-lysine coated cell culture plate in RPMI XF media (Agilent) supplemented with 10mM glucose (Agilent ...
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bioRxiv - Genomics 2023Quote: ... 0.9 μl of ‘11.1x buffer’ [21] and 0.06 μl of a 20:1 mix of PCRBIO Taq Polymerase (PCRBiosystems; 5U/μl): Pfu DNA polymerase (Agilent Technologies; 2.5 U/μl). PCR cycling conditions for the amplification of cat mitogenome segments generally consisted of an initial denaturation at 96°C for 1 min ...
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bioRxiv - Microbiology 2023Quote: ... using two different carrier gases (argon and helium) and two stationary phases (CP- Molsieve 5 Å Plot and PoraPLOT U J&W GC columns, Agilent Technologies, Germany). Moreover ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 U/μl Murine RNase inhibitor (Vazyme, Cat. No. R301-03) and 2% AffinityScript Multiple Temperature Reverse Transcriptase (Agilent, Cat. No. 600100-51). After incubation of 5 min at 65°C of RNA with random hexamers ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in quadruplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Plant Biology 2020Quote: ... Eluting metabolites were fragmented in electron impact mode with an ionization voltage of 70 eV and data was acquired using MSD ChemStation software (revision D.01.02.SP1, Agilent Technologies). Analytes were identified based on their mass fragmentation patterns by comparison with those of authentic standards using the National Institute of Standards and Technology Mass Spectral Search Program (version D.05.00) ...
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bioRxiv - Immunology 2022Quote: ... Seahorse XF Cell Culture Microplates were pre-treated with poly-D-lysine and 106 primary B cells or 105 DG75 cells were plate in Seahorse XF Base Medium (Agilent) supplemented with 2mM L-glutamine (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were analyzed with Qubit and fragment size was accessed using the high sensitivity D-5000 screen tapes (5067-5592 Agilent) on a Tapestation.
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... 60sal- MUT2 and 60sal-MUT3 were generated from pENTR/D-TOPO-UAS(5x)-60sal(1x) using Pfu polymerase and Quick Change Site-Directed Mutagenesis (Stratagene). Sequences of 6 nucleotides were changed C to A and G to T using the oligonucleotide pairs 60sal- MUT1 Fw and 60sal-MUT1 Rv ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 HO-1 +/+ and HEK293 HO-1 -/- were seeded at a density of 2.5 × 104 cells/well on a poly-D-lysine (#P6407-5MG) coated Seahorse XF96/XF Pro cell culture microplate (#204624-100, Agilent) on the day prior to the analysis ...
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... T140A/D SETD8 cDNAs were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (Agilent #210518).
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bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Physiology 2022Quote: ... The LC-MS/MS data acquired using Agilent Mass Hunter Workstation (.d files) were processed in quantitative analysis software (Agilent Technologies) for quantitative analysis of samples ...
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bioRxiv - Microbiology 2021Quote: ... the fractions were injected on HPLC for purification performed on an Eclipse+ C18 column (L = 150 mm, D = 3.0 mm, Particles diameter 5 µm) (Agilent, Waldbronn, Germany). The volume injected was 100 µl ...
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bioRxiv - Neuroscience 2024Quote: ... d) using a Cascade Summit series semi-automated probe station connected to a 4294A precision impedance analyser (Keysight, Agilent Technologies, model). The measurements were carried out by measuring the total impedance between the counter electrode ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Cell Biology 2024Quote: ... The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent, USA). All measurements were performed using Seahorse XFe96 Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... oligomycin (1 µM) and 2-deoxy-D-glucose (2-DG) (50 mM) (Seahorse Glycolysis Stress Test Kit, Agilent Technologies, 103020-100). Analyses were performed with Wave software 2.3.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Immunology 2024Quote: B cells were treated with asparaginase for 18h and 1.5x105 cells were plated on a poly-D- lysine coated 96-well Seahorse utility plate (Agilent, California, United States). Metabolic function was analysed by measuring basal metabolism and metabolism after oligomycin ...
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bioRxiv - Microbiology 2021Quote: The determination conditions of bacillomycin D and fengycin were established by injecting 10 μL samples into an HPLC column (Eclipse XDB-C18, 5 μm; Agilent, Santa Clara, CA). The temperature was maintained at 30 °C during the experiment ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Bioengineering 2023Quote: ... The optical purity of L-lactic acid and D-lactic acid were measured via a HPLC system (Agilent 1260 series, Hewlett-Packard, USA) equipped with a SCAS Sumichiral OA-5000 column (150 × 4.6 mm ...
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bioRxiv - Immunology 2024Quote: ... of the V(D)J libraries was quantified on an Agilent TapeStation 4150 using a D5000 ScreenTape and Reagents (Agilent, 5067-5588, 5067-5589), while the concentration (ng/uL ...
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bioRxiv - Plant Biology 2024Quote: ... The identification and quantification of volatile metabolites was carried out using an Agilent Model 8890 GC and a 7000 D mass spectrometer (Agilent, Palo Alto, CA, USA), equipped with a 30 m × 0.25 mm × 0.25 μm DB-5MS (5% phenyl-polymethylsiloxane ...
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bioRxiv - Genomics 2020Quote: ... Blots were washed three times with TBST for 15min at room temperature and subsequently incubated for 2h at room temperature with HRP-conjugated secondary antibodies (goat anti-rabbit, Dako; rabbit anti-goat, R&D Systems) at a dilution of 1:5000 in TBST ...