Labshake search
Citations for Agilent :
901 - 950 of 6656 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... PCR3 products were PCR-purified and Tapestation (Agilent) was used to quantify and pool samples for NGS.
-
bioRxiv - Plant Biology 2021Quote: ... in the AriaMx Realtime PCR System (Agilent Technologies) as previously described (Arsova et al. ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitive PCR was performed using the AriaMX (Agilent) with a 25 µl reaction ...
-
bioRxiv - Microbiology 2020Quote: ... Mutations were generated by PCR using Quikchange (Stratagene). SdhA deletions were generated by inverse PCR (Ochman et al. ...
-
bioRxiv - Microbiology 2023Quote: ... PCRs were performed with Stratagene Mx3005P (Agilent Technologies) with a 0.04 threshold ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4C PCR library efficiencies were confirmed by Agilent Bioanalyzer and libraries were sequenced on a HiSeq4000 in PE150 mode by the MSKCC Genomics Core Facility ...
-
bioRxiv - Plant Biology 2023Quote: ... The PCR reactions used Pfu Turbo Cx (Agilent), dNTP (Takara Bio) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The PCR samples were also analyzed by Agilent 2100 Bioanalyzer system (Agilent Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... prior to PCR analysis following manufacturer’s guidelines (Agilent). Gene-specific TaqMan probes (Applied Biosystems ...
-
bioRxiv - Cell Biology 2023Quote: ... mutations were corrected by PCR-based mutagenesis (Stratagene). Secondly ...
-
bioRxiv - Zoology 2019Quote: ... with a flame ionization detection (FID) and fatted with DB-FF column (30m×0.25mm, diameter = 0.5μm, Agilent Technologies, USA). Nitrogen was supplied as the carrier gas at a flow rate of 40 mL/min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of primary antibodies (pan-Cytokeratin and α-SMA) was performed by using EnVision-mouse HRP (Dako, Hamburg, Germany) for 45 min at RT ...
-
bioRxiv - Physiology 2020Quote: ... Separation and detection of total triglyceride FAMEs was achieved using a 6890N Network GC System (Agilent Technologies; CA, USA) with flame ionization detection ...
-
bioRxiv - Synthetic Biology 2020Quote: ... This was followed by incubation and wash steps (four washes) and final detection with 100 µL of 3,3’,5,5’-tetramethylbenzidine (TMB) and Substrate-Chromogen (Dako, Denmark). The reaction occurred for 30 s and then was stopped by the addition of 50 µL of 1 M H2SO4 per well ...
-
bioRxiv - Cancer Biology 2020Quote: ... The spectrophotometric data were obtained using a Synergy 2 Multi-Detection Microplate Reader (Agilent Technologies, Santa Clara, CA, USA) at a wavelength of 450 nm ...
-
bioRxiv - Cell Biology 2020Quote: ... The reactions for K14 were detected with EnVision™ FLEX/HRP Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) and visualized with Magenta Substrate chromogen system (Agilent/Dako ...
-
bioRxiv - Cell Biology 2020Quote: ... The reactions were visualized using EnVision™ FLEX /HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+GV809/821) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The reactions for K14 were detected with EnVision™ FLEX/HRP Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) and visualized with Magenta Substrate chromogen system (Agilent/Dako ...
-
bioRxiv - Cell Biology 2020Quote: ... The reactions were visualized using EnVision™ FLEX /HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+GV809/821) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... The slides were washed again in PBS and then incubated with the streptavidin-peroxidase (DAKO Detection System - K0690, USA). The reactions were revealed with DAB (K3468 ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by washing and detection of bound Adu with a rabbit anti-mouse horse radish peroxidase conjugated antibody (Dako) and 3,3′,5,5′-tetramethylbenzidine substrate ...
-
bioRxiv - Neuroscience 2021Quote: ... TH immunolabeling with horseradish peroxidase (HRP) - 3,3-diaminobenzidine (DAB) detection and hematoxylin counterstain was performed using an Dako Omnis automated machine (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
-
bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
-
bioRxiv - Biophysics 2021Quote: Room-temperature (RT) absorption spectra of cells were acquired on a Varian Cary 4000 UV–Vis-spectrophotometer (Agilent technologies) equipped with an integrating diffuse reflectance sphere (DRA-CA-50 ...
-
bioRxiv - Neuroscience 2020Quote: ... slides were washed again and incubated for 30 min at RT with Envision+ HRP labelled polymer anti-mouse (DAKO). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... pLAIΔEnvGFP.Gag-LUC RT D185E and INT D116N were generated by site-directed mutagenesis using Pfu Turbo DNA polymerase (Agilent) and the following primers:
-
bioRxiv - Neuroscience 2023Quote: ... cDNA of the first RT reaction as well as the final library was QC’ed via High Sensitivity DNA chip bioanalyzer (Agilent).
-
bioRxiv - Microbiology 2023Quote: ... 12µl of sample was incubated with 2µl of 25µM AR2 primer at 72°C for 2min using 10x AffinityScript RT buffer and enzyme (Agilent Technologies), 0.1M DTT ...
-
bioRxiv - Cell Biology 2020Quote: ... Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023). Blocking was done in M.O.M ...
-
bioRxiv - Developmental Biology 2021Quote: ... then at 4°C overnight with primary antibodies diluted in Dako REAL Antibody Diluent (Agilent, Santa Clara, CA). We used the following primary antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Then slides were incubated with E2F1 (VD301790, Thermo Fischer, 1:100 diluted in Dako REAL Antibody Diluent S2022) or E2F8 antibody (ab185727 ...
-
bioRxiv - Systems Biology 2019Quote: ... 20% of the total mixture was incubated at 37°C with PNGaseF and fractionated into 16 high pH reversed phase fractions using a 1260 Infinity II HPLC (Agilent Technologies, Santa Clara, CA) with a 4.6 × 150 mm XBridge C18 column and a 30 minute gradient (mobile phase A ...
-
bioRxiv - Molecular Biology 2019Quote: ... of each lipid class were prepared by acid-catalyzed transesterification at 50°C for 16 hours (Christie, 1973) before quantified by a Agilent 7890B gas chromatograph with flame ionization detector (Agilent Technologies, Santa Clara, CA).
-
bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
-
bioRxiv - Cell Biology 2021Quote: ... or IRBITKO cells was subjected to PCR amplification (Herculase II Fusion DNA Polymerase and 5X Herculase II PCR Buffer and dNTP (Agilent Techologies) using primers flanking the region targeted by the gRNA ...
-
bioRxiv - Immunology 2020Quote: ... qRT-PCR for mRNA expression was done with the Brilliant III Ultra-Fast SYBR Green qRT-PCR Master Mix (Agilent Technologies) as previously described (19) ...
-
bioRxiv - Genomics 2019Quote: ... were washed three times with 200 μl binding buffer (SureSelect Target Enrichment, Agilent Technologies), before incubation with the Hi-C DNA/RNA capture bait mixture with 200 μl binding buffer for 30 min at room temperature ...
-
bioRxiv - Genetics 2021Quote: ... They were then washed three times and mounted in mounting medium (DAKO, ref #S3023) containing 1µg/mL DAPI or incubated with Phalloidin 633 (1:1000 in 1X-PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 2 times with XF DMEM assay medium (Agilent, #103575-100), supplemented with glucose (Agilent ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Samples were diluted 4 times in water and ran on a Bioanalyzer (Agilent Technologies) to determine cDNA concentration ...
-
bioRxiv - Biochemistry 2022Quote: ... washed three times with PBS and mounted onto slides using fluorescence mounting medium (Dako).
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 100 µL of XF Base Media (Seahorse Bioscience) containing 2 mM L-Glutamine ...
-
bioRxiv - Immunology 2023Quote: ... the sections were washed three times with PBS and incubated with HRP polymer (DAKO) for 30 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... washed again three times in PBST and mounted using Mowiol mounting medium (Dako, #S3023). Rabbit anti-TH (1:1000 ...
-
bioRxiv - Biochemistry 2023Quote: ... system coupled with an quadrupole time of flight mass spectrometer (Agilent 6545, QTOF-MS). Analytes were separated on a C18 bridged ethylene hybrid column (Waters ...
-
bioRxiv - Plant Biology 2023Quote: ... coupled with a quadrupole time of flight mass spectrometer (Agilent 6546 LC/Q-TOF) operating in positive ionization mode (Agilent Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... and rinsed a final time with PBS before mounting with fluorescence mounting medium (Dako). The slides were then imaged using widefield or confocal microscopy.
-
bioRxiv - Plant Biology 2023Quote: ... EO was diluted 1000 times in pentane and subjected to GC-MS analysis (Agilent Technologies 7980[A gas chromatograph system with the 5977[A mass selective detector) ...