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Citations for Agilent :
901 - 950 of 2715 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were depleted semi-automatically with an Agilent Multiple Affinity Removal Column Human 14 column (MARS Cat# 5188-6557, Agilent Technologies, USA) on a microflow HPLC system using MARS buffers A and B in a gradient as suggested by the manual ...
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bioRxiv - Genomics 2024Quote: ... Labelling and hybridization of the patient’s and reference DNA (#5190-3797, Human 230 Reference DNA Female, Agilent Technologies, Santa Clara, CA, USA) were performed using enzymatic labelling and hybridization protocols,v.7.5 (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: Array-based comparative genomic hybridization (aCGH) was performed using SurePrint G3 Human CGH 8×60K microarrays (Agilent Technologies, Santa Clara, CA, USA) with 41 kb overall median probe spacing according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... 60,000 cells were plated on XF24 cell plates (Agilent) to measure cell respiration ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells (15,000 cells/well) were plated on an XF96 polystyrene cell culture microplate (Seahorse®, Agilent). The following day ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were plated at confluency (40,000 cells per well) in Seahorse XF96 Cell Culture Microplates (Agilent). Cells were transfected with siRNA or mammalian expression vectors at the time of plating (reverse transfection) ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C in primary antibody (2% NGS in TBST) with either rabbit anti-GFAP (1:2000, Agilent Cat# Z0334, RRID: AB_10013382), rabbit anti-synaptophysin (IgG ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were fixed in fixed in 4 % of paraformaldehyde in phosphate-buffered saline (PBS) before permeabilization with 0.5% Triton X-100 in PBS and stained with primary antibodies: anti-Ki67 (M7240, Agilent Dako, Santa Clara, CA, USA) diluted at 1/50 and anti-Poly-ubiquitin purchased from ENZO (ENZ-ABS840 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lifted and seeded at 2×104 cells/well in XFe96 Cell Culture Microplate (Agilent Technologies) in 80 μl of fresh medium ...
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bioRxiv - Immunology 2023Quote: Caco-2 cells were plated at 30,000 cells per well on Seahorse XFe24 Cell Culture Microplate (Agilent). At the end of the experiment ...
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bioRxiv - Biochemistry 2022Quote: ... cells were seeded at 10,000 cells/well in 96 well Seahorse XF cell culture plates (Agilent, UK). The sensor cartridges were hydrated overnight with tissue culture grade H2O in a non-CO2 incubator at 37°C as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto Seahorse XFe96 cell culture plate (Agilent) at a density of 6000 - 8000 cells/well with culture medium for 24 hours ...
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bioRxiv - Physiology 2022Quote: ... cells were seeded on XF96 cell culture microplates (Seahorse Bioscience) at a seeding density of 5.0 x 103 cells per well ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated in XF96 well cell culture microplates (Agilent) at equal densities in substrate-limited medium (DMEM with 0.5 mM glucose ...
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bioRxiv - Molecular Biology 2023Quote: After culturing cells on Seahorse XFe96 cell culture microplates (Agilent), the medium was replaced with Seahorse XF DMEM (Agilent ...
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bioRxiv - Cell Biology 2019Quote: One-color microarray-based gene expression analysis was performed using a SurePrint G3 Human Gene Expression v3 8×60K Microarray Kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Neuroscience 2021Quote: The exome sequences were enriched from 1.0 μg genomic DNA using an Agilent liquid capture system (Agilent Sure Select Human All Exon V5). First ...
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bioRxiv - Cell Biology 2019Quote: ... KLF4 and C-MYC open reading frames under the control of the human elongation factor-1α (EF-1α) promoter in the presence of 1.2% GeneJammer (a polyamine-based transfection reagent; Stratagene, La Jolla, CA, USA). Cells were maintained in mTeSR™1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Hybridization of samples was conducted on Agilent Whole Human Genome 4×44-k 60-mer oligonucleotide arrays (G4112F, Agilent Technologies, Santa Clara, CA), using 150ng of total RNA as template/sample ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Cancer Biology 2024Quote: ... WES libraries were prepared and captured using an Agilent SureSelect Human All Exon kit (Catalog No. 5191-5735, Agilent Technologies, Santa Clara, CA). The DNA library ...
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bioRxiv - Neuroscience 2020Quote: ... the myoblasts were incubated overnight at 4 °C with primary antibodies against sarcomeric myosin, MF-20 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:200) and Desmin (Dako, Santa Clara, CA, 1:100). After washing with PBS ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products, catalog number GA64461-2). However ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Genomics 2023Quote: RNA seqFISH+ and two-layer DNA seqFISH+ primary probes were generated from oligo array pools (Twist Bioscience for 150-nt oligos; Agilent Technologies for 170-nt oligos) based on Oligopaint technologies80 with enzymatic amplifications as described previously7 ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Agilent). Cells were grown to optical density at 600nm (OD600 ...
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bioRxiv - Cell Biology 2019Quote: AD293 cells (Agilent) were maintained in Dulbecco’s Modified Eagles medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (Stratagene) with an N-terminal TEV cleavable GST tag ...
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bioRxiv - Biophysics 2021Quote: ... AD293 cells (Agilent) were cultured in DMEM basic (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Agilent) were transformed with pET28-S plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Agilent) were transformed with ~2 μg αHL-D8H6 plasmid40 without heat shock and incubated on LB-agar plates containing 25 g L−1 lysogeny broth (LB ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Agilent). An overnight starter culture of cells was grown in 2xYT supplemented with carbenicillin (50 μg/L ...
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bioRxiv - Plant Biology 2021Quote: ... coli cells (Stratagene) and purified using an amylose resin according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (Agilent) were transformed with the plasmid ...