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Citations for Agilent :
901 - 950 of 1825 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The plate was immediately loaded into a Biotek Synergy 4 96-well plate reader (Agilent Technologies, USA), and NADPH fluorescence (340/460 excitation/emission ...
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bioRxiv - Microbiology 2023Quote: ... TIMS dimension was calibrated linearly using 4 selected ions from ESI Low Concentration Tuning Mix (Agilent Technologies) [m/z ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C with anti-total tau primary antibody solution (1: 5000, DAKO A0024). After 1 hour incubation at RT in the corresponding secondary antibody solution (donkey anti-rabbit 800) ...
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bioRxiv - Microbiology 2024Quote: ... 4-CP and phenol were measured using a high-Performance Liquid Chromatograph (HPLC, model: 1100, Agilent, USA) equipped with a 250 nm diode array detector (DAD ...
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bioRxiv - Genomics 2024Quote: ... Chromatin was collected by centrifugation (14,000xg, 10 minutes at 4C) and quantified (Qubit 4 Fluorometer, Agilent TapeStation). Native H3K4me3 ChIP-seq was performed in 500ul SDSless RIPA [10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Unstained 4-m thick sections were labeled with H&E or anti-CD79a (1:2000, Dako M7050) and anti-CD56 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemical reaction was developed using 3, 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cell Biology 2020Quote: ... Frozen samples were spread to a thin layer on petri dishes pre-cooled on dry ice and UV-crosslinked 3 times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). All samples were stored at −80°C until tissue lysis.
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Developmental Biology 2020Quote: ... the included linker sequence between 3’ UTR and the start of sfGFP was removed using site-specific mutagenesis (QuikChange, Agilent). The gRNA expression plasmid and donor plasmid were injected by BestGene into embryos of Vas-Cas9 flies (#56552 ...
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bioRxiv - Genetics 2021Quote: ... Additional sequences were added to the 5’ (ACTGGCCGCTTGACG-) and 3’ (-CGCAGGAGCCGCAGTG) ends of each fragment and synthesized in a 100K pool by Agilent Technologies (Supplementary Table S2) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times 10 min in Tris-Triton Solution and mounted on gelatin-coated slides using Fluorescence Mounting Medium (Dako). Z-stack images (4 optical sections ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The purified PCR products were subsequently used as megaprimers to generate the library of mutants in the plasmid pZE-ProQ-3×FLAG by following GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) guidelines ...
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bioRxiv - Microbiology 2020Quote: ... was generated by introducing mutations in the 3’ splice site of the pPOLI-WSN-M plasmid using QuikChange II site-directed mutagenesis protocol (Agilent). pPolI-WSN-M-M2SMRtoAAA (Mut1) ...
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Microbiology 2021Quote: ... and then with streptavidin-HRP complex followed by 3,3-diaminobenzidine or 3-amino-9-ethylcarbazole detection (LSAB kit, Dako France). Sections were then counterstained with hematoxylin ...
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bioRxiv - Plant Biology 2021Quote: ... and 3 μl were injected in a splitless mode into an Agilent Gas chromatography mass spectrometry (GC-MS) system (Agilent 7890A chromatograph ...
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bioRxiv - Plant Biology 2021Quote: ... The Zorbax Eclipse Plus C-18 column Rapid Resolution HT (3 X 100 mm, 1.8 μm, 600 bar, Agilent, USA) was kept at 25°C and the elution was performed with acetonitrile (Optima ...
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bioRxiv - Molecular Biology 2020Quote: ... The free HSF2BP protein as well as the complex between ARM and F15X were analyzed using a Bio SEC-3 column (Agilent) equilibrated in 25 mM Tris-HCl buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Bisulfite-converted DNA was used for PCR amplification of the DMRs and products were visualized on a 3% Tris-Acetate-EDTA agarose gel and quantified by TapeStation (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... were changed to 5′-ACCTGCAAAG −3′ by using the Quick-Change site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). Expression vectors for SENP2 ...
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were then washed for 3 min in TBST and incubated for 30 min with HRP (Horse Raddish Peroxidase) conjugated anti-rabbit (Ref: K4003, Dako) or anti-mouse (Ref ...
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bioRxiv - Neuroscience 2022Quote: ... A frequency of 59.673 MHz and an amplitude of 8 dBm was generated by a signal generator (EXG Analog Signal Generator, NS171B, 9 kHz – 3 GHz, Agilent) and fed into the transmitter and reception boards of the scanner via a respective custom made power splitter.5
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bioRxiv - Biochemistry 2022Quote: UPLC separations were performed on a reverse phase Poroshell 120 EC-C18 column (3 × 100 mm, 2.7 μm) (Agilent Technologies) operating at 30 °C and a flow rate of 0.4 mL/min ...
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bioRxiv - Cancer Biology 2022Quote: ... For signal detection the MACH 3 Mouse HRP Polymer Detection system was employed according to manufacturer’s protocol using DAB+ (Fa.Agilent Technologies, K3468). Slides were counter-stained with Hematoxylin Gill’s Formula (Fa.Vector ...
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bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
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bioRxiv - Neuroscience 2020Quote: ... DI rinse and wash buffer prior to a 3-minute incubation with Haematoxylin ready-to-use solution (K8018, Agilent DAKO) and a further DI rinse and wash step ...
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bioRxiv - Neuroscience 2020Quote: ... DI rinse and wash buffer prior to a 3-minute incubation with Haematoxylin ready-to-use solution (K8018, Agilent DAKO) and a further DI rinse and wash step ...
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared for each time-points and seady-state sample using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) automated on the NGS WorkStation (Agilent) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were de-waxed and antigen retrieval was performed in a pressure cooker at 125°C for 3 min employing an Antigen Retrieval solution (Dako, pH6 for EZH2 ...
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bioRxiv - Microbiology 2022Quote: ... 50 mg/mL glycogen (1/50 vol/vol and 3 volumes of 100% ethanol. The quality of the isolated RNA was assessed on an Agilent Bioanalyzer ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into pVp16-Dest vector for IPTG (isopropyl-β-thiogalactopyranoside)-induced expression (3 h at 37°C) in E.coli strain ArcticExpress (Agilent). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... Then all membranes were washed 3 times and mounted on coverslips using an aqueous fluorescent mounting medium (DakoCytomation, Huddinge, Sweden). Samples were analysed with Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... SPEC-PTSCX sample cleanup pipette tips and Bond Elut C18/ 3 mL cleanup cartridges were from Agilent (Santa Clara, CA), cell culture slides (8-chamber ...
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bioRxiv - Developmental Biology 2021Quote: ... pCS2+-5’HA-sobp and pCS2+-sobp-3’HA were generated using the QuikChange lightning Site-directed mutagenesis kit (Agilent). The same kit was used to sequentially remove three nucleotides located at the 5’ end of the pCS2+-3’HA-sobp ORF to generate a construct whose transcribed mRNA does not bind to the designed translation-blocking antisense morpholino oligonucleotide (pCS2+-sobpMOI-3’HA ...
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bioRxiv - Cell Biology 2020Quote: ... 6 PCR reactions including two biological replicates and 3 technical replicates per gene were performed using Brilliant SYBR master mix (Agilent), running cycles of 10 minutes at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: Primary brown adipose cells were isolated and cultured for 3 days before plated in XF cell culture microplates (Seahorse Bioscience). Cells (10,000 cells ...
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bioRxiv - Microbiology 2020Quote: ... and ZIKV X1 C35G R (5’-GCCTGCTAGTCAGGCACAGCTTGGGGA-3’) were used with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) to introduce the X1 C10415G mutation into the p2-ZIKV plasmid ...
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bioRxiv - Genetics 2020Quote: Single variants were introduced by mutagenesis of the Tile 3-KCNH2 plasmid (Table S1) using the Quikchange Lightning Multi kit (Agilent), with 1 primer per variant ...
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p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional responsebioRxiv - Cancer Biology 2021Quote: ... The cells were washed 3 times with PBS for 5 min and then incubated with a secondary antibody: Flex-HRP (EnVision System, Dako) for 30 min and washed 3 times 5 min with PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Phosphorylation site mutations and gRNA sequences were introduced into vectors by PCR amplification with mutagenic primers (Table 3) with Phusion (Thermo) or PfuUltra II (Stratagene) polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... Glacial acetic acid was added to dissolve the crystals and the absorbance was measured at 595 nm with a Cytation 3 Image Reader (Agilent). The values of vehicle-treated controls were set to 100%.