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Citations for Agilent :
901 - 950 of 1018 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and anti-GGCT (16257-1-AP; proteintech) antibodies were used for immunodetection on rehydrated sections pretreated with Target Retrieval Solution (S1699; DAKO). The Envision™+ system (HRP/DAB ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were washed twice in TTBS buffer before incubation in a diluted solution of Fluorescein (FITC) conjugated F (ab’)₂ fragment goat anti-rabbit IgG (Dako) or Alexa Fluor 647 conjugated F(ab)2 fragment swine anti-rabbit secondary antibody (Molecular Probes) ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then heated to 97°C for 20 minutes in a high pH target retrieval solution (TRS) (K8004, Agilent) for heat-induced antigen retrieval (HIER) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC staining were loaded into an Agilent pre-treatment module to be dewaxed and undergo heat induced epitope retrieval (HIER) using high pH target retrieval solution (TRS) (K8004, Agilent). The sections were heated to 97⁰C for 20 minutes in high pH TRIS buffer ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by adding STOP solution and the absorbance at 450 nm was recorded using a BioTeck Gen5 plate reader (Agilent). Endpoint titers were calculated as the dilution that emitted an optical density exceeding 4X the PBS control group.
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bioRxiv - Immunology 2023Quote: ... reaction was stopped by adding STOP solution and absorbance at 450 nm was recorded using a BioTeck Gen5 plate reader (Agilent). Endpoint titers were calculated as the dilution that emitted an optical density exceeding 4X the PBS control group.
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bioRxiv - Immunology 2023Quote: ... reaction was stopped by adding STOP solution and absorbance at 450 nm was recorded using a BioTeck Gen5 plate reader (Agilent). For nucleocapsid ...
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bioRxiv - Developmental Biology 2023Quote: For IHC staining slides were thawed and antigen-retrieval performed by incubating the sections with preheated Target Retrieval Solution (DAKO) 20 min at 70°C ...
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bioRxiv - Immunology 2023Quote: For CFTR detection in cross sections, target retrieval was performed with a citrate buffer (Target Retrieval solution, pH 6, (Dako) for 30min after deparaffinization ...
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bioRxiv - Cell Biology 2022Quote: ... Immediately after the slides were immersed in pre-heated (90-100 degree Celsius) 1X antigen retrieval solution (Dako, cat # S1699) for 25 min ...
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bioRxiv - Bioengineering 2023Quote: ... The clarified aqueous solution was filtered using a 0.22 µm filter and run on HPLC-RID (Agilent 1290 Infinity II/Agilent 1100) for glucose measurements ...
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bioRxiv - Bioengineering 2024Quote: Near-IR absorption spectroscopy: The NIR absorbance spectra of solutions were measured using a UV-Vis-NIR spectrophotometer (Cary 5000, Agilent). During the measurement ...
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bioRxiv - Plant Biology 2024Quote: ... The internal reference masses of 121.050873 m/z and 922.009798 m/z (G1969-85001 ES-TOF Reference Mass Solution Kit, Agilent Technologies & Supelco) were used for all analyses of the samples ...
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bioRxiv - Cell Biology 2024Quote: ... the culture medium was changed with the assay medium containing unbuffered DMEM supplemented with 10 mM Seahorse XF glucose solution (Agilent), 1 mM Seahorse XF pyruvate solution (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Deparaffinization and antigen retrieval was performed in the low pH EnVision FLEX Target Retrieval Solution at 98 °C for 24 minutes in the DAKO PT link from Agilent. Antibodies were diluted in DaVinci Green Diluent (Biocare ...
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bioRxiv - Genomics 2024Quote: ... slides were rinsed three times in milli-Q water before submerging into bluing buffer (Bluing Solution, Cat No. CS702, Dako). Slides were washed once in milli-Q water ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by a double wash with PBS and with distilled water and mounting on slides using a mounting solution (Dako).
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bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
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bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
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bioRxiv - Cancer Biology 2024Quote: ... with 0.1% v/v Ammonium Hydroxide (Alfa Aesor) in 90% Water (Honeywell)/10% Acetonitrile (BioSolv) containing 5 µM Infinitylabs Deactivator Solution (Agilent). Solvent B was 90% Acetonitrile / 10% Water containing 5 µM Infinitylabs Deactivator Solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed at 95-98°C for 30 min using 1X of Target Retrieval Solution pH9 (S236784-2, Agilent). The tissue slides were cooled down for 20 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Staining solution was once again rinsed and replaced with PBS and cell count was analyzed using a Biotek Cytation1 (Agilent) measuring puncta count at Ex.377 ...
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bioRxiv - Plant Biology 2020Quote: ... sucked 500 μl solution from the tube to detect the concentrations of remaining nitrate using High Performance Capillary Electrophoresis System (Agilent Technologies). Then measured the root fresh weights and calculated the nitrate uptake speeds (NO3− (μmol)/root fresh weight (g)/h) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Cell Biology 2022Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 37°C in a non-Co2 incubator and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Genomics 2020Quote: ... gDNA before SRE treatment was diluted 5-fold and the undiluted solution after SRE treatment was used according to the protocol using TapeStation 2200 (Agilent Technologie) and Genomic DNA ScreenTape (Agilent Technologie).
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bioRxiv - Pathology 2020Quote: ... The quantity and quality of the RNA solution was assessed using a Qbit machine and an Agilent 2100 Bioanalyzer (Agilent Technologies) before library construction and sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... The release process of photo-uncaged lifitegrast from the contact lens to its bathing solution was monitored using UV-vis absorption spectroscopy recorded by an Agilent 8453 UV–vis spectrophotometer (Agilent Technologies).
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The mass analyzer was calibrated between m/z 118 and 2721 using an Agilent ESI-L low concentration tuning mix solution (Agilent, USA) at a resolution of 20 000 giving a mass accuracy below 2 ppm ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). After processing of raw data as previously described24 ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
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bioRxiv - Plant Biology 2022Quote: ... 100μL of hybridization solution was dispensed into the gasket slide and assembled to the Agilent SurePrint HD Arabidopsis GE 4X44K Microarrays (Agilent®). Slides were incubated for 17 h at 65°C in an Agilent hybridization oven ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Formalin-fixed samples tissue microarrays of EwS-samples and normal tissues were stained for GPR64 after antigen retrieval using Target Retrieval Solution (Fa.Agilent Technologies, S1699) with anti-GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Neuroscience 2021Quote: Paraffin-embedded sections were deparaffinized and autoclaved at 121°C for 20 min in Target Retrieval Solution (Dako Japan Inc., S1700). Frozen sections were briefly washed in PBS and incubated with 0.5 % SDS (v/v ...
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bioRxiv - Pathology 2022Quote: ... paraffin embedded (FFPE) tissue sections were wax removed with Xylene and incubated in Target retrieval solution pH 9.0 (Dako, Glostrup, Denmark) for 10 min at room temperature prior to incubating for 15min at 110°C ...
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The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligamentsbioRxiv - Molecular Biology 2020Quote: ... 30μg of the soluble fraction was subjected to an in-solution digestion on 10 μl Strataclean™ resin (Agilent Genomics, UK) followed by reduction and alkylation in 3 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2020Quote: ... The solution was filtered and then injected into LC-MS system (Agilent 6538 UHD-QTOF equipped with Agilent 1290 infinity UPLC). Upon extracting the chromatograms based on the reported m/z ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed for 15 min in the autoclave (250°F) using 1x Target antigen retrieval solution (Dako; S169984-2). All antibody steps were performed as described above ...
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bioRxiv - Immunology 2021Quote: ... Slides were then rinsed with double distilled water (ddH2O) and boiled in 1X Dako pH9 antigen retrieval solution (Agilent, S236784-2) for 10 min ...