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Citations for Agilent :
901 - 950 of 1351 citations for 4 2 Trimethylsilyl Ethyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
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bioRxiv - Cell Biology 2024Quote: ... and HP-0921 from Agilent (m/z 922.0098) in positive mode and 4-NBA (m/z 166.0146) and HP-0921 from Agilent (m/z 966.0007, formate adduct) in negative mode.
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bioRxiv - Systems Biology 2024Quote: ... The sequencing libraries were quantified using the Qubit 4 fluorometer and library sizes were quantified using the D1000 ScreenTape assay (Agilent 5067-5582) on the TapeStation 4200 automated electrophoresis platform.
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bioRxiv - Biophysics 2024Quote: A 60,000 member tiling library of the Disprot database (release 2022 12) containing 16 residue-long peptides with 4-fold overlap was ordered from Agilent (G7270A, USA). The library was solubilised in 40 µL TE buffer yielding ∼1 µM concentration ...
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bioRxiv - Bioengineering 2024Quote: ... 12 by PCR using primers 3 and 4 listed in Supplementary Table 1 and cloned into the BamHI-KpnI site of pBluescript II KS (+) (Stratagene, CA, USA) using ligation mix (Takara Bio USA) ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies (Supplementary Table 2) were diluted according to manufacturer’s guidelines in DAKO Antibody Diluent (cat. S202230, Agilent). Cells were incubated with the primary antibody mixes overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... we generated a pT7-D6/2-NSP1-null via the QuikChange II site-directed mutagenesis kit (Agilent Technology) based on pT7-D6/2-NSP1 (22) ...
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bioRxiv - Biochemistry 2020Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Cat. N. P044701-2, Agilent Technologies LTD, Cheadle, UK) (2.4μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... The tissues were deparaffinized to distilled water and the antigen retrieval performed with Proteinase K (Dako #S302030-2) treatment for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions contained 10 μL of 2×Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent), 2 μL of each 2 μM primers (Table S5) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 µl aliquot of the sample was injected into LC-QTOF-MS system (Agilent, 6545 LC/QTOF-MS) coupled with a C18 column (ZORBAX RRHD Plus C18 ...
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bioRxiv - Plant Biology 2021Quote: ... All mutations listed in Supplemental Table 2 were introduced using the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent) with appropriate mutagenic primers ...
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bioRxiv - Plant Biology 2021Quote: ... Purified peptide suspensions (2 μL each) were injected into a HPLC-chip (Polaris-HR-Chip-3C18, Agilent Technologies) through a capillary pump with a flow at 1.5 μL/min ...
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bioRxiv - Microbiology 2021Quote: ... and the quality was checked on 2% agarose gel and verified using the Agilent Bioanalyser 2100 (Agilent technologies). RNAs selected for further analysis had a minimum RNA Integrity Number (RIN ...
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bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with primary antibodies: rabbit polyclonal anti-PSMA/GCPII (1:150) (M362029-2; DAKO, US), anti-CD68 (1:250 ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with appropriate primary antibodies diluted in EnVision FLEX Antibody Diluent (Agilent Dako, K800621-2) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Genomics 2020Quote: The Aβ coding sequence and two flanking regions of 52bp and 72bp respectively upstream and downstream of Aβ were amplified (primers MS_01 and MS_02, Supplementary file 2) by error-prone PCR (Mutazyme II DNA polymerase, Agilent). 30 cycles of amplification and 0.01ng of initial template were used to obtain a mutagenesis rate of 16 mutations/kb ...
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bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
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bioRxiv - Immunology 2021Quote: A pAAV-MCS plasmid containing inverted terminal repeats (ITRs) from AAV serotype 2 (Agilent Technologies, Santa Clara, CA) was utilized as backbone for AAV6 plasmid construction (naturally occurring AAV6 has an AAV2 ITR (24)) ...
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bioRxiv - Cell Biology 2021Quote: ... T-cell lymphoma diagnoses were confirmed by immunohistochemical staining of sections with anti-CD3 antibody (Agilent, M725429-2) and DAB solution development.
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bioRxiv - Microbiology 2022Quote: ... pneumoniae was done in 96 well microtitre plates using a BioteK Epoch-2 microtiter plate reader (Agilent Technologies). Each treatment was replicated in at least 3 wells ...
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bioRxiv - Developmental Biology 2022Quote: ... Repeated washes were performed before incubation with secondary antibody (FITC-labelled swine anti-rabbit F (ab’)2 (Dako) or Alexa Fluor 647-labelled goat anti-rabbit IgG (Molecular Probes ...
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bioRxiv - Physiology 2022Quote: ... were placed in 50 µl XF medium (non-buffered DMEM containing 10 mM glucose and 2 mM GlutaMax, pH 7.3-7.4, Agilent), centrifuged in a carrier tray (300g for 3 min with no break) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was then performed at 97 °C for 10 minutes with Target Retrieval Solution (Agilent, S236784-2) on a PT Module (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
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bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Bioengineering 2022Quote: ... before incubating in primary antibody diluted in DAKO antibody diluent (Cat# S080983-2, Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2-hour conjugation of 12.0 μM streptavidin (Prozyme, SA10) and 100 μM fluorescent dye (Alexa Fluor 488 Cadaverine or Lissamine Rhodamine B ethylenediamine ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were steamed for 35 minutes with a pH 6 Dako Target Retrieval (Agilent Technologies, S169984-2) and permeabilized with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
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bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies against human CD3 (1:100; #A045229-2) and CD68 (1:400; #M0876) were obtained from Dako, UK ...
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bioRxiv - Microbiology 2024Quote: ... sections were blocked for 1 h at room temperature in serum-free blocking buffer (Agilent Technologies, X090930-2), and then incubated with polyclonal rabbit antibody to ASBT (kindly provided by Paul Dawson ...
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bioRxiv - Genetics 2024Quote: ... PCR-2 reactions amplified vector fragments from MSp509_PKR-WT plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kb ...
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bioRxiv - Immunology 2024Quote: ... then in 3% hydrogen peroxide for 15min and in serum-free protein block solution (Dako, Cat # X090930-2) for 30min ...
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bioRxiv - Microbiology 2024Quote: ... washed 3x with dPBS and resuspended at 2% hematocrit in Seahorse XF DMEM assay medium (pH 7.4) (Agilent) containing 10 mM glucose (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit TMEM119 at 1:500 (ab185333, Abcam, mouse PG-M1 at 1:400 (GA61361-2, Dako Omnis, Agilent), mouse P2RY12 at 1:1000 (ab180366 ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit TMEM119 at 1:500 (ab185333, Abcam, mouse PG-M1 at 1:400 (GA61361-2, Dako Omnis, Agilent), mouse P2RY12 at 1:1000 (ab180366 ...