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Citations for Agilent :
851 - 900 of 1345 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Site-directed mutagenesis was performed by PCR techniques using PfuUltra high-fidelity DNA Polymerase (Stratagene). All mutations were confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using the PfuUltra II Fusion High-fidelity DNA polymerase (Agilent, California, USA) using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen ...
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bioRxiv - Microbiology 2020Quote: ... Viral cDNA was amplified by PCR using the Herculase II polymerase (Agilent, Santa Clara, CA) and amplified viral cDNA was gel purified using a Spin Gel Purification kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: In-vitro mutagenesis by PCR-mediated recombination QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) was performed using the cDNA of human GPRC6A cloned in the vector pcDNA3 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... and cloned into a plasmid vector using the StrataClone blunt PCR cloning kit (Agilent; 240207). Colonies were selected and grown in 4ml 2xTY media supplemented with Ampicillin (10μg/ml) ...
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bioRxiv - Developmental Biology 2022Quote: ... Amplified fragments were then cloned into StrataClone PCR Cloning Vector pSC-A-amp/kan (Stratagene) and sequenced in order to check for the identity and orientation of the inserted PCR fragment ...
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bioRxiv - Evolutionary Biology 2022Quote: ... vi) PCR enrichment and index ligation using Kapa HiFi Hot Start Uracil+ Ready Mix (Agilent) for bisulfite libraries (14 cycles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were tested for Mycoplasma contamination using MycoSensor PCR Assay Kit (Agilent, Didcot, UK).
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bioRxiv - Molecular Biology 2020Quote: ... The plate was then loaded into an Agilent AriaMax real time PCR machine (Agilent Technologies) and the qRT-PCR was performed (1 cycle at 95.0°C for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were cloned into a pCMV-Tag2 plasmid (Stratagene, San Diego, CA, USA) digested with BamHI and SalI containing FLAG-tag and S-tag sequences ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were visualized using Agilent Tape Station system (Agilent Technologies, Palo Alto, CA, USA), yielding a band of 81 bp for a methylated product and 93 bp for an unmethylated product ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed using a Stratagene QPCR Systems Mx3005P (Agilent Technologies, Santa Clara, CA, USA) using the QuantiTect SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Cell Biology 2019Quote: ... we used Roche’s LightCycler and Brilliant II SYBR® Green QRT-PCR Master Mix (Agilent).
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bioRxiv - Developmental Biology 2020Quote: ... The Dot1L sequence was subcloned by PCR amplifying and inserting into CTAP vector from Agilent, as well as pGEX-4T-3 from GE.
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bioRxiv - Cell Biology 2020Quote: ... TC10 mutants were produced through PCR-based site-directed mutagenesis using the Quikchange kit (Stratagene). For the Q75L mutation ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed using the Brilliant III Ultra-Fast SYBR® dye system (Agilent) with ROX as a reference dye ...
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bioRxiv - Pathology 2021Quote: ... A linear range of values was obtained for PCR amplification on a Mx3000 Thermocycler (Agilent) using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... SEP-GluA1-S845A was generated by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed mutagenesis were performed via PCR following the QuikChange site-directed mutagenesis protocol (Agilent) and using PfuUltra II Fusion HS DNA Polymerase (Agilent) ...
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bioRxiv - Genetics 2019Quote: ... The PCR product was ligated with T4 ligase prior to transformation into competent cells (Stratagene). The plasmid was sequenced to validate the removal of the putative EGR1 binding site ...
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bioRxiv - Developmental Biology 2021Quote: ... The purified PCR reaction was checked on the Bioanalyzer using High Sensitivity DNA chip (Agilent). Size selection of the sRNA library was done on LabChip XT instrument (Perkin Elmer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cloned into pSC-A-amp/kn using a StrataClone PCR Cloning Kit (Agilent Technologies). Plasmids were then Sanger sequenced using T7/T3 primers or the original PCR primers (MWG Eurofins) ...
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bioRxiv - Neuroscience 2021Quote: ... PCR-based mutagenesis kit exactly as described by the manufacturer (Stratagene, La Jolla, CA, USA). Mutagenized clones were isolated and subjected to Sanger DNA sequencing to verify the entire coding sequence for the presence of the desired mutation and the absence of any PCR-induced sequence errors.
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bioRxiv - Cell Biology 2020Quote: ... cDNA was prepped by reverse-transcriptase PCR using the AffinityScript cDNA QPCR Synthesis kit (Agilent Technologies ...
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bioRxiv - Genetics 2019Quote: ... The PCR products obtained were analyzed by migration on a 2,100 Bioanalyzer instrument (Agilent Technology).
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bioRxiv - Molecular Biology 2022Quote: The ABE7.10 mutant library was generated with error prone PCR using GeneMorph II (Agilent #200550) in the high mutation rate condition as described in manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplified PCR products were separately gel purified and ligated into blunt-end digested pBlueScript (Stratagene). Sanger nucleotide sequencing analysis was performed on DNA from 48 transformed colonies using a T7 primer.
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bioRxiv - Microbiology 2022Quote: ... Quantitative qRT-PCR was performed with the Brilliant II SYBR Green QPCR Master Mix (Agilent). Primers were designed to amplify ∼150 bp product within the target genes ...
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bioRxiv - Molecular Biology 2022Quote: ... The libraries were amplified with 7 PCR cycles using Herculase II Fusion Polymerase kit (Agilent). Libraries hybridisation was performed as described previously 68 using probes mapping to 122 polycomb target genes promoters and 78 control active gene promoters ...
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bioRxiv - Plant Biology 2022Quote: ... Transcript quantification was carried out using Stratagene MX3000P real-time PCR detection system (Agilent Technologies) with FIREPol® EvaGreen® Mix (Solis BioDyne) ...
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bioRxiv - Genomics 2023Quote: ... the PCR was performed in duplicate per ligation reaction using Herculase II reagents (Agilent Technologies). The parallel library preparations and PCR reactions were subsequently pooled for each reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product size distribution was checked using Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) after adapter ligation ...
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bioRxiv - Genomics 2023Quote: ... The resulting PCR product was sent for analysis using a 5200 Fragment Analyzer System (Agilent) and the quantification for each PCR fragment is given for each sample ...
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bioRxiv - Physiology 2023Quote: ... qRT-PCR was carried out with the Agilent MX3000 system (Agilent, Santa Clara, California, USA), using the THUNDERBIRD SYBR qPCR Mix (ToYoBo ...
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bioRxiv - Biochemistry 2022Quote: The selected nucleotide sequence was amplified by PCR using Herculase II Fusion Enzyme (Agilent, USA) with specific oligonucleotide primer pairs incorporating p15TV-L adapters ...
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bioRxiv - Physiology 2023Quote: ... Error prone PCR mutagenesis was performed using the GeneMorph II Random mutagenesis kit (Agilent, Germany). Standard PCR reactions for cloning or site-mutagenesis were done using Herculase II Fusion polymerase (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were performed using a Pfu-Ultra II Fusion High Fidelity DNA Polymerase (Agilent). All sequences were verified using Sanger sequencing (Microsynth) ...
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bioRxiv - Plant Biology 2023Quote: ... PCR amplification was carried out using Herculase II Fusion DNA Polymerase enzyme (Agilent Genomics, USA) with specific primers (Supplemental Table S1).
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were subjected to thermal denaturation in a Real Time PCR machine (Agilent Stratagene Mx3005P). Fluorescence intensity of the probe was recorded throughout denaturation using a temperature gradient from 25 °C to 95 °C ...
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bioRxiv - Neuroscience 2023Quote: qRT-PCR reactions were performed using Brilliant II SYBR Green qPCR Master Mix (Agilent #600828) according to manufacturer instructions using a Light Cycler HT7900 (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were purified using the AMPure XP system and quality was assessed by Agilent Bioanalyzer 2100 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were subjected to thermal denaturation in a Real Time PCR machine (Agilent Stratagene Mx3005P). Fluorescence intensity of the probe was recorded throughout denaturation using a temperature gradient from 25 °C to 95 °C ...
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bioRxiv - Immunology 2024Quote: PCR was carried out with an error-prone polymerase (Agilent, GeneMorph II Random Mutagenesis Kit) to create a randomly mutagenized GP38 library as previously described79.
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bioRxiv - Cancer Biology 2023Quote: Each library preparation PCR reaction contained the following components: 1μl Herculase II Fusion Enzyme (Agilent), 2.5μl Deoxynucleotide (dNTP ...
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bioRxiv - Neuroscience 2024Quote: ... PCR reactions were performed using a Pfu-Ultra II Fusion High Fidelity DNA Polymerase (Agilent), whereas Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were tested for mycoplasma contaminants using a PCR-based detection kit (Agilent). LN-95 AR knockout cells were provided by Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... and heated for 3 minutes (35°-80°C) in a PCR machine (Agilent SureCycler 8800). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... and heated for 5 minutes (40°-70°C) in a PCR machine (Agilent SureCycler 8800). Next ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PCR was performed on a StraTGene Mx 3005P qPCR system (Agilent Technologies, Santa Clara, CA). The PCR program heats to 95°C for 10 min and conducts 45 cycles of 15 s at 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... Low ROX (95074; Quanta BioSciences) in a Stratagene Mx4000 multiplex quantitative PCR system (Agilent Technologies) using primers for Drosophila 18S and 28S rRNA ...