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Citations for Agilent :
851 - 900 of 7100 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Quantitative detections of 15N2 and 15NO were performed by GC/MS (model 7890A/5975C, Agilent) equipped with a CP-Molsieve 5A Plot (25 m×0.32 mm×30 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... chromogen detection (with HRP polymer, anti-rabbit or anti-mouse, with Envision System from Dako) and hematoxylin counterstaining were performed per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021, les Ulis - France) utilizing onboard Dako Omnisautomate OMNIS (Dako ...
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bioRxiv - Plant Biology 2020Quote: ... Separation and detection was next performed using an Agilent 1200 series HPLC system (Agilent Technologies) as previously reported (Fraser et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... chromogen detection (with HRP polymer, anti-rabbit or anti-mouse, with Envision System from Dako) and hematoxylin counterstaining were performed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibody followed by 10 min incubation with the DAB substrate/chromogen detection system (DAKO). The sections were counterstained for 2 min with hematoxylin (0.1%) ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection was performed by horseradish peroxidase (HRP)-conjugated secondary antibodies (all from Agilent, CA, USA) and chemiluminescence (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... for 5 mins before adding the detection system reagent Streptavidin-HRP (DAKO Cat Number P0397) 1:500 in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were performed using Easy-A High-Fidelity PCR master mix (Agilent Technologies, UK) on a Mastercycler Pro Thermal Cycler (Eppendorf ...
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bioRxiv - Microbiology 2019Quote: ... Both PCR amplifications were carried out using the Paq5000 Hotstart PCR Master Mix (Agilent, USA). The library was sequenced on the MiSeq platform with the MiSeq Reagent V2 250 cycles kit ...
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bioRxiv - Microbiology 2020Quote: ... All primary and secondary antibodies were diluted in Dako REAL antibody diluent (Agilent technologies). Images were captured and analysed using a Nikon Eclipse TI microscope with NIS-Elements Microscope Imaging Software (Nikon).
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bioRxiv - Molecular Biology 2023Quote: ... sections were further blocked with Dako REAL Peroxidase-Blocking solution (Agilent, Santa Clara, USA) for 20 min ...
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bioRxiv - Pathology 2023Quote: ... We blocked against unspecific antigens by applying DAKO Real blocking solution (DAKO, Cat# S2023) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... We blocked against unspecific antigens by applying DAKO Real blocking solution (DAKO, Cat# S2023) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... or E2F8 antibody (ab185727, Abcam, 1:500 diluted in Dako REAL Antibody Diluent S2022) overnight or for 60min ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5 ...
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bioRxiv - Microbiology 2021Quote: ... at RT for 1 h followed by incubation with streptavidin R-Phycoerythrin (PE, Agilent Technologie) at RT for 30min ...
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bioRxiv - Neuroscience 2019Quote: ... consisting of a 10-minute RT incubation with boiling antigen retrieval solution (Dako, Ely, UK). Slides were washed three times with PBS and blocked for 1 hour with 5% Normal Donkey Serum (NDS ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min at RT and peroxidase activity was revealed using DAB+ (Dako, K346811-2). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 hours at RT before mounting them in clear mounting medium (DAKO, cat#S3023). We imaged fluorescent signals of mounted brain sections under a scanning microscope (ZEISS ...
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bioRxiv - Cell Biology 2022Quote: ... at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent). Primary antibodies used herein are listed in Table 2 while secondary antibodies used are ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene, La Jolla, USA). Up to eight colonies containing the PCR product were selected and inoculated on Luria Bertani agar slants ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Bioengineering 2020Quote: ... LEU4 and Ll_ilvD were generated by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). The CEN plasmids harboring wild-type ILV6 (pYZ127) ...
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bioRxiv - Plant Biology 2021Quote: ... Grown colonies were screened with colony PCR using CP-F and UnivR primers and KAPA2G Robust HotStart Kit (Agilent, Supplemental Method 2). Sanger sequencing of the selected clone confirmed correct sequence of the PVY-coding part and correct in-frame insertion of GFP coding sequence ...
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bioRxiv - Cell Biology 2022Quote: ... All other constructs were made by PCR amplification followed by standard restriction enzyme cloning or by site-directed mutagenesis kit (QuickChange II, Agilent Technologies, 200523). All oligonucleotides were obtained from Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... The original eGFP-C2-DEF6 plasmid (5) was used to generate the mutant DEF6 proteins by mutagenesis PCR (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent Technologies, #210516). All mutations were verified by sequence analysis (see Suppl ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Probes produced from PCR templates were radiolabelled with [α-32P]-dCTP (Hartmann Analytic, #SRP-305) using Prime-it II Random Primer Labelling kit (Agilent, #300385) and oligonucleotides used as a probe were radiolabelled with [γ-32P]-ATP (Hartmann analytic #SRP-501) ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Molecular Biology 2023Quote: ... the A3 sequence specifying amino acids 21-393 was amplified from pHD1344tub(PAC)-Nterm3V5-A3 by error prone PCR using the GeneMorphII Random Mutagenesis kit (Agilent, Cat # 200523) and primers that add AttB sites (Table S2 ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Cell Biology 2020Quote: Quantitative reverse transcription PCR (qRT-PCR) analysis was carried out on an MX300-P (Agilent technologies) using gene specific primers (QuantiTect® ...
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bioRxiv - Biochemistry 2023Quote: ... cells were washed three times with seahorse medium (Agilent Technologies, 102353) containing 2 mM glutamine ...
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bioRxiv - Microbiology 2024Quote: ... retention times for each component were confirmed using MS (Agilent 6490) with no iodine treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma Plus PCR Primer Set (Agilent) was used to determine mycoplasma for all cell lines ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... PCR was performed using Mx3005P (Agilent) with SYBR® Premix EX TaqTM II (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... and detection was carried out at 254 nm in a photodiode array detector (G1315D, Agilent Technologies). The mobile phase consisted of a filtered and degassed solution of 0.1 M NaH2PO4 0.2 mM EDTA pH 3.1 (Harapanhalli et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Absorbance was measured at 564 nm with a Synergy 2 Multi-Detection Microplate Reader (Agilent Technologies) and normalized to a vehicle control ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were re-suspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cancer Biology 2021Quote: Tumor MMR status was determined by immunohistochemical detection of PMS2 (anti-PMS2 antibodies; clone EP51, DAKO) and MSH6 (anti-MSH6 antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The detection was carried out using Dako EnVision®+ Dual Link System-HRP (DAB+) (Dako, Agilent). The Liquid DAB+ Substrate Chromogen System (Dako ...
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bioRxiv - Microbiology 2023Quote: ... The detection was carried out using Dako EnVision®+ Dual Link System-HRP (DAB+) (Dako, Agilent). The Liquid DAB+ Substrate Chromogen System (Dako ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed with a secondary Ab conjugated to HRP (Agilent DAKO, Santa Clara, CA, USA) and visualized with tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed with a secondary Ab conjugated to HRP (Agilent DAKO, Santa Clara, CA, USA) and visualized with tetramethylbenzidine (TMB ...
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bioRxiv - Bioengineering 2023Quote: ... followed by detection using similar protocols published previously(21) with the NovoCyte flow cytometer (Agilent, USA). The iFluor 647 fluorescent intensity was detected with the APC channel ...
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bioRxiv - Cancer Biology 2022Quote: Drug detection studies were performed using Accurate Mass-Q-TOF mass spectrometer (Agilent Technologies Inc, USA) equipped with electron spray ion source (ESI) ...