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Citations for Agilent :
851 - 900 of 1173 citations for Recombinant Human IL18RAP Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Whole-exome sequencing libraries were prepared using SureSelectXT Human All Exon V5 + UTR kit (75 Mb; Agilent technologies, Santa Clara, CA, USA) as per manufacturer’s instructions and sequenced on HiSeq 2000 to generate paired end 2 × 100bp sequence reads (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Exome and clinical exome sequencing was performed on 3μg of genomic DNA using the Agilent SureSelectXT Human All Exon (V6) and Focussed Exome respectively (Agilent Technologies, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the 3 liver cancer specimens also underwent bulk-level WES using Agilent SureSelect Human All Exon v7 K it (Agilent, 5191-4005) and illumina NovaSeq 2 × 150 bp sequencing mode ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were depleted semi-automatically with an Agilent Multiple Affinity Removal Column Human 14 column (MARS Cat# 5188-6557, Agilent Technologies, USA) on a microflow HPLC system using MARS buffers A and B in a gradient as suggested by the manual ...
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bioRxiv - Genomics 2024Quote: ... Labelling and hybridization of the patient’s and reference DNA (#5190-3797, Human 230 Reference DNA Female, Agilent Technologies, Santa Clara, CA, USA) were performed using enzymatic labelling and hybridization protocols,v.7.5 (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: Array-based comparative genomic hybridization (aCGH) was performed using SurePrint G3 Human CGH 8×60K microarrays (Agilent Technologies, Santa Clara, CA, USA) with 41 kb overall median probe spacing according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949 ...
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bioRxiv - Genetics 2023Quote: Exome enrichment was applied to the cDNA purified from the previous step by using probe kit SSELXT Human All Exon V8 (Agilent, 5191-6879) for human samples ...
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Genomics 2021Quote: Exome capture was carried out for six cell lines and 39 mixtures using SureSelect Human All Exon V6 (Agilent Technologies, Inc., CA, USA). To minimize duplicate reads in ultra-deep sequencing ...
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bioRxiv - Cell Biology 2019Quote: One-color microarray-based gene expression analysis was performed using a SurePrint G3 Human Gene Expression v3 8×60K Microarray Kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Neuroscience 2021Quote: The exome sequences were enriched from 1.0 μg genomic DNA using an Agilent liquid capture system (Agilent Sure Select Human All Exon V5). First ...
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bioRxiv - Cell Biology 2019Quote: ... KLF4 and C-MYC open reading frames under the control of the human elongation factor-1α (EF-1α) promoter in the presence of 1.2% GeneJammer (a polyamine-based transfection reagent; Stratagene, La Jolla, CA, USA). Cells were maintained in mTeSR™1 (STEMCELL Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The reaction products for the total RNA of the human cell line KMM-1 were analysed by microfluidics-based automated electrophoresis with the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... WES libraries were prepared and captured using an Agilent SureSelect Human All Exon kit (Catalog No. 5191-5735, Agilent Technologies, Santa Clara, CA). The DNA library ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Cancer Biology 2023Quote: ... Overexpjression of MTf was accomplished by stably transfecting SK-MEL-28 cells with human MTf cDNA or with the empty pCMV-Script® (Stratagene, CA, USA) vector alone14 ...
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bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Hybridization of samples was conducted on Agilent Whole Human Genome 4×44-k 60-mer oligonucleotide arrays (G4112F, Agilent Technologies, Santa Clara, CA), using 150ng of total RNA as template/sample ...
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bioRxiv - Cancer Biology 2024Quote: DNA libraries were prepared from tumor and matched normal tissues of ACCC patients using Low Input Exome-Seq Human v6 (Agilent Technologies, Waldbronn, Germany) for exome capture and analyzed on a NovaSeq 6000 (Paired-End 150bp S2 ...
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bioRxiv - Genomics 2021Quote: ... using a mixture of equal volume of ready-to-use protein block serum-free solution (Agilent Dako ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were submitted to the Stanford Protein and Nucleic Acid Facility for quality assessment by Agilent Bioanalyzer to determine the RNA integrity (RIN ...
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bioRxiv - Developmental Biology 2020Quote: ... the slides were further washed and blocked in protein block serum-free buffer (Dako, Cat # X0909). The slides were incubated with AIBP antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... then stained with: rabbit polyclonal (pAb) glial fibrillary acidic protein (GFAP,1:1000, Dako, Z033401-2), goat pAb glial fibrillary acidic protein (GFAP,1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... The labeled proteins were subjected to digestion with Sialidase A (Agilent Technologies, Santa Clara, CA, USA), β1-4 galactosidase (Agilent Technologies) ...
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bioRxiv - Neuroscience 2019Quote: Primary antibodies used for immunofluorescence were polyclonal rabbit anti-glial fibrillary acidic protein antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation in 3% hydrogen peroxide and in serum-free protein block solution (Dako, X0909) before adding primary antibody for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The molecular weights for each rNA were estimated using an AdvanceBio SEC 300Å protein standard (Agilent) of known molecular weights that was included in the run and the presence of NA in each fraction was measured with the Mu-NANA assay.
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bioRxiv - Microbiology 2021Quote: ... Separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies). Samples were loaded on a 100 µm × 20 mm trap column (in-house packed with ReproSil Pur C18-AQ ...
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Long-term alterations in brain and behavior after postnatal Zika virus infections in infant macaquesbioRxiv - Neuroscience 2019Quote: ... we performed immunohistochemical (IHC) analysis by using antibodies against Glial fibrillary acidic protein (Agilent Technologies, M0761), NeuN (EMD Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µg of anti-HA antibody (12CA5) was immobilized on Protein A cartridges (Agilent, G5496-60000), using the in-built immobilization methodology ...
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bioRxiv - Microbiology 2021Quote: ... the protein was digested by trypsin overnight and the digested peptides were purified with ZipTips (Agilent). Using a Dionex UltiMate 3000 RSLCnano system equipped with a Dionex UltiMate 3000 RS autosampler ...
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bioRxiv - Neuroscience 2020Quote: ... Peptide/protein identifications and quantitative analysis were performed using Spectrum Mill (Agilent Technologies, Santa Clara, CA) as described previously ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mL fractions were collected and protein was concentrated by incubation with 4μL StrataClean resin (Agilent Technologies) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and slides were incubated with Dako Serum Free Protein Block (#X090930-2, Agilent, Santa Clara, CA) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... FcγR binding was quantified by incubating immune complexes with biotinylated FcγRs (FcγRIIB, FcγRIII, and FcγRIV, courtesy of Duke Protein Production Facility) conjugated to Steptavidin-PE (Prozyme). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Biophysics 2021Quote: ... Separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies). Samples were loaded on a 100 µm × 20 mm trap column (in-house packed with ReproSil-Pur C18-AQ ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigens (for HE1-, or LL-) proteins were detected using 10 mg tablets of DAB (Dako Cytomation) diluted in 5 ml Tris-buffer (brown colour).
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bioRxiv - Neuroscience 2022Quote: ... washed in PBS (3 × 5min) and incubated with serum free protein block (Dako, Cat# X090930-2) for 1h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated for 1 hour at room temperature with serum-free protein block (Dako X0909) to block non-specific binding sites followed by Cleaved-Casp3 primary antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP) antibody (RRID:AB_10013382; Dako Agilent, Santa Clara, CA, USA, #z0334), mouse anti-DCX (RRID:AB_10610966 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP) antibody (RRID:AB_10013382; Dako Agilent, Santa Clara, CA, USA, #z0334), mouse anti-DCX (RRID:AB_10610966 ...