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Citations for Agilent :
851 - 900 of 2041 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide microarrays for human whole genome (G4858A design 072363, 8×60k chips SurePrint G3 unrestricted GE, Agilent Technologies) were used for global gene expression analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 2009). MEKK1 542–888 construct (H. sapiens MEKK1 TOG) was amplified from the MegaMan human transcriptome library (Agilent). The TOG construct was cloned into pETNKI-his-3C-LIC-kan vector ...
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bioRxiv - Molecular Biology 2019Quote: ... The R1285Q mutant of human FANCI (FANCImut) was generated by using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), and was cloned with a N-terminal cleavable Flag tag into the pCDNA 3.1 vector ...
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bioRxiv - Genetics 2019Quote: ... and exome DNA was enriched with an Agilent SureS elect Human All Exon V5 kit (Agilent Technologies, USA). Paired-end sequencing was performed on Illumina HiSeq 4000 (Supplementary Table 1 and 2).
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bioRxiv - Physiology 2021Quote: Mutations were introduced into human Cx50 cDNA in a SP64T vector with QuikChange Site-Directed Mutagenesis Kit (Stratagene) by using PCR primers that amplified the whole plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and incubated with horse radish peroxidase (HRP) conjugated goat-anti-human IgG antibody (Dako, P0447) (diluted 1:1,600 in PBS/0/1% BSA ...
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bioRxiv - Genetics 2020Quote: ... The whole exome was captured by an Agilent SureSelect All Human exon v5.0 kit (Agilent, Santa Clara, US.), and sequenced on an Illumina Hiseq X-10 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
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bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Cancer Biology 2023Quote: ... which recognises residues 1978-2016 of human NaV1.5) and stained with EnVision+ Dual Link System/HRP (DAB+; Dako), following the manufacturer’s instructions and counterstained with Mayer’s haematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Quick Amp Labeling Kit and SurePrint G3 Human Gene Expression 8×60Kv3 Microarray (Cat. No. G4851C, Agilent Technologies) was used corresponding to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Positive control PhIP-seq designed to capture mouse and human GFAP began by incubating 0.1µg/mL polyclonal anti-GFAP antibody (Dako) with the murine PhIP-seq library.
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The sequence library was prepared using a Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced using a 2500 Illumina with 125-bp paired-end reads (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated at 95°C for 30 minutes in Antigen Retrieval Solution (pH 9) (Dako), cooled down at RT and rinsed in TBS ...
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bioRxiv - Immunology 2019Quote: ... for 12h at 37°C before immunostaining according to standard protocol with anti-CD3 (Polyclonal; Dako), anti-CD8 (clone 4B11 ...
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bioRxiv - Genetics 2021Quote: ... containing the c.547G>A mutation introduced by site-directed mutagenesis (QuickChange Lightning kit, Agilent Technologies), and a pgk-neor selection cassette flanked by FRT sites ...
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bioRxiv - Microbiology 2020Quote: ... Acetylene and ethylene were detected by flame ionisation (FID) at 300°C and ChemStation software (Agilent) was used to integrate signal peak areas ...
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bioRxiv - Cancer Biology 2020Quote: ... then sections were incubated overnight with primary antibodies at 4°C with Background Reducing Components (DAKO). Alexa Fluor secondary antibodies (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... NSPCs where incubated for 1 h in a non-C02 but humidified 37 °C incubator (Agilent) in the same XF assay medium as described above ...
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bioRxiv - Pathology 2021Quote: Mitochondrial respirations were measured at 37°C in a Seahorse XF24 Extracellular Flux Analyzer (Agilent, USA). The XF Cell Mito Stress Test Kit (103015-100 ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 20 minutes at 4°C before mounting with Dako Fluorescence Mounting Medium (Agilent) and glass coverslips ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: FFAs in liver tissue were extracted and measured by GC-MS (Agilent 7890 A / 5975 C) according to the method described by Tang et al(26) ...
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bioRxiv - Physiology 2019Quote: ... at 95°C for 20 min in a high pH buffer (Dako Antigen retrieval, high pH). Endogenous peroxidase was blocked using the EnVision FLEX Peroxidase-Blocking kit followed by two washes for 5 min each (Dako wash buffer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated from 25 to 96°C in a Mx3005P real-time PCR instrument (Stratagene). Fluorescence was monitored with excitation and emission filters set to 465 and 590 nm ...
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bioRxiv - Biochemistry 2021Quote: ... proSP-C BRICHOS D105N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with rabbit anti-GFAP (1:500; DAKO, Z0334). The antibody was prepared in 10% donkey serum in PBS containing 0.02% sodium azide and 0.3% triton X-100 ...
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bioRxiv - Immunology 2019Quote: ... for 12h at 37°C before immunostaining according to standard protocol with anti-CD3 (Polyclonal; Dako), anti-CD4 (clone 1F6 ...
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bioRxiv - Biophysics 2022Quote: ... Data acquisition and analyses were performed using OpenLAB CDS ChemStation Edition C.01.05 (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
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bioRxiv - Microbiology 2023Quote: ... with agitation at 37°C for 16 hours in an imaging multimode reader (Cytation 5, Agilent). Optical density at 600 nm (OD600) ...
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bioRxiv - Neuroscience 2023Quote: ... Partial purification was achieved using C-18 solid phase extraction columns (Agilent Technologies, Lake Forest, CA). A series of 4 elutions with 1.5 mL of 60% ...
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bioRxiv - Neuroscience 2023Quote: ... Mutation of Syngap1 (SYNGAP1-c.2214_2217del) was introduced by site-directed mutagenesis (QuikChange Lightning, Agilent #210518). HEK293T cells were co-transfected with expression vectors using Lipofectamin 3000 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... at 4 °C ON and afterward incubated with rabbit anti-HA antibody 1:2000 (Dako, A0001) 2 h at RT ...
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genomics 2019Quote: ... 37,608 individual biotinylated RNAs targeting the ends of 22,076 promoter-containing human HindIII restriction fragments) were prepared according to the manufacturer’s instructions (SureSelect Target Enrichment, Agilent Technologies). The Hi-C library DNA was denatured for 5 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Genetics 2019Quote: ... DNA libraries were prepared by using the SureSelect Human All Exon V6 kit (Agilent Technologies, Santa Clara, CA, USA) and were paired-end sequenced on the Illumina HiSeq 4000 platform at 100X coverage ...
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bioRxiv - Genetics 2021Quote: Oxford Nanopore MinION long-read sequencing was performed on RNA isolated from an adult human cortex (540005, Agilent Technologies). PolyA-enriched RNA was isolated using the Dynabeads mRNA Direct kit (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome-sequencing libraries were generated using the Agilent SureSelect Human All Exon V4+UTR capture platform (Agilent, CA, USA). Libraries were sequenced to a target depth of 200-fold coverage on a 100 bp paired-end sequencing run using an Illumina HiSeq 2000 instrument ...
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bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...