Labshake search
Citations for Agilent :
8451 - 8500 of 9267 citations for Human Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 0.3 μg of total RNA using the Affinityscript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, US). qPCR was performed using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA extraction was performed using a FinePure Plant RNA Kit (Genfine Biotech Co. Ltd., Beijing, China) and the qualities were assessed using a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA). A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA integrity (RIN) was assessed using a Bioanalyzer 2100 instrument in conjunction with the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California).
-
bioRxiv - Plant Biology 2022Quote: ... The RNA quality was assessed using both the NanoPhotometer ® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Genomics 2022Quote: ... RNA samples were then quantified and checked for RNA integrity using an Agilent BioAnalyzer 2100 with RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA), and only samples with an RNA integrity number ≥8 were sent for RNA-Seq ...
-
bioRxiv - Cell Biology 2022Quote: ... I15K/R54P) were generated by mutagenesis of pcDNA-3XFLAG-TRIM25 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA), while the TRIM25-PTAA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values were > 7 for all samples ...
-
bioRxiv - Neuroscience 2021Quote: ... The quality and quantity of the extracted RNA samples were analyzed with a 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA). Paired-end cDNA libraries were prepared from 0,2 ng of extracted RNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Samples from Tenebrio molitor and Calliphora vicina were qualified with the High Sensitivity RNA ScreenTape Assay Kit and the Agilent 4200 TapeStation system (Agilent, Santa Clara, United States) and Pyrrhocoris apterus samples with the Agilent RNA 6000 Pico Kit on a BioAnalyzer 2100 (Agilent ...
-
bioRxiv - Immunology 2022Quote: ... Individual indexed IGK and IGL AIRR-seq libraries were assessed using the Agilent 2100 Bioanalyzer High Sensitivity DNA Assay Kit (Agilent, Cat. No. 5067-4626) and the Thermofisher Qubit 3.0 Fluorometer dsDNA High Sensitivity Assay Kit (Thermofisher ...
-
bioRxiv - Neuroscience 2022Quote: ... cDNAs were synthesized through reverse transcription from the total RNA according to the protocol of Affinity Script Multiple Temperature cDNA synthesis kit (Agilent Technologies, Santa Clara, CA). Expression levels of genes encoding plp (forward primer ...
-
bioRxiv - Plant Biology 2019Quote: ... Additional synthetic SlMai1 variants were generated by PCR amplification using oligonucleotides encoding the amino acid changes followed either by BP Clonase recombination (for G2A and C3/4S mutants) or using the QuickChange Site-Directed Mutagenesis kit (for K91M; Agilent, Santa Clara, CA, USA).
-
bioRxiv - Cancer Biology 2019Quote: ... we modified the PTK-ATF4-Luc plasmid from (Dey et al., 2010)39 using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent; Santa Clara, California, USA) to introduce two BsmBI restriction sites flanking the ATF4 5’ UTR ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
-
bioRxiv - Cancer Biology 2020Quote: ... Target capture were performed on pools of 96 libraries using a Sure Select® user-defined probe kit (Agilent Technologies, Palo Alto, CA, USA). Massively parallel sequencing was carried out in a High-Seq® machine (Illumina ...
-
bioRxiv - Cancer Biology 2019Quote: The pEGFP-N1-CELF1 putative eIF4E binding mutants were generated via site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, La Jolla, CA) and primers listed in Supplementary Table 2 ...
-
bioRxiv - Developmental Biology 2019Quote: We used the manufacturer protocol for the Agilent SureSelect Capture kit for non-human exomes with 200 ng gDNA per individual (75 Mb capture designed on the zv9 version of the zebrafish genome; Agilent SSXT Zebrafish All Exon kit; Agilent Technologies, Santa Clara, CA). Samples were multiplexed and run across two lanes of the Illumina HiSeq 4000 as paired-end 150 bp reads ...
-
bioRxiv - Neuroscience 2019Quote: ... and SNAREQ (SNAP25 K40A/D51A/E52A/E55A/D166A) were generated in the above described Syt1 and t-SNARE background respectively using the QuickChange mutagenesis kit (Agilent Technologies, Santa Clara, CA). All proteins were expressed and purified as described elsewhere (Mahal et al ...
-
bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
-
bioRxiv - Microbiology 2021Quote: ... The purity and integrity of the RNA pools were checked by the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit and Bioanalyzer2100 system (Agilent Technologies, CA, USA). One microgram of RNA from each pool was used for library preparation with the NEBNext® UltraTMRNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... plasmid phapXVENUS-hph (Gsaller et al., 2014) was used as template and modified using QuikChange II site-directed mutagenesis kit (Agilent for K38E and R50A) or Q5 site-directed mutagenesis kit (New England Biolabs ...
-
bioRxiv - Neuroscience 2021Quote: ... The fragment distribution of each library was assessed the using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA High Sensitivity kit (ThermoScientific ...
-
bioRxiv - Immunology 2021Quote: cDNA was synthesized from the previously extracted RNA of mice colon tissue by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random 9mer primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... after the molecular cloning the wt sequence was restored by mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, CA), the pETM11-CspA plasmid as DNA template and the mutagenic primers G670 5′-TTTCAGGGCGCCATGTCCGGTAAAATGACTG-3′ and G671 5′-CAGTCATTTTACCGGACATGGCGCCCTGAAA-3′.
-
bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... HMW DNA was also imaged with a gDNA 165 kb kit (FP-1002-0275) on the Femto Pulse system (Agilent, Santa Clara, CA, USA). A detailed procedure is deposited in protocols.io (dx.doi.org/10.17504/protocols.io.bafmibk6).
-
bioRxiv - Cancer Biology 2021Quote: ... purity and integrity using NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit of Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Samples were pooled to satisfy the quantity criteria of RNA sequencing and a minimum amount of 20 ng RNA per pooled sample was used as input material for the RNA sample preparations ...
-
bioRxiv - Neuroscience 2020Quote: The α10W55T mutant subunit was produced as previously described (Boffi et al., 2017) using Quick change XL II kit (Stratagene, La Jolla, CA, USA). The chimeric α10x construct was generated in two steps ...
-
bioRxiv - Microbiology 2022Quote: ... The final library concentration was measured using the Qubit dsDNA HS Assay Kit (Thermo Fischer Scientific, USA) and the average library size was estimated using the Bioanalyzer TapeStation 2200 (Agilent Technologies, Santa Clara, USA). The libraries were diluted to 4 nM stocks ...
-
bioRxiv - Genomics 2022Quote: ... Quantity were assessed on a Qubit 2.0 Fluorometer using a Qubit RNA HS Assay kit and the quality were checked by capillary electrophoresis on an Agilent Bioanalyzer using the RNA 6,000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA). Purified RNA was stored at −80 °C until further use.
-
bioRxiv - Neuroscience 2022Quote: ... These constructs were made by performing site-directed mutagenesis on the Syt9 cDNA with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA). Full-length CLM constructs were used for the rescue experiments in Fig ...
-
bioRxiv - Cancer Biology 2022Quote: We performed in-solution targeted enrichment of exonic sequences from spleens of two heterozygous MVD013 mice on C3H background together with six mutants and two wild-type controls from three other lines on the same genetic background using the SureSelectXT Mouse All Exon 50 Mb kit (Agilent Technologies, Santa Clara, CA). The generated libraries were indexed ...
-
bioRxiv - Immunology 2022Quote: ... and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Cell Energy Phenotype kit on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The quality of extracted RNA was verified using the Agilent 2100 Expert Bioanalyzer using the RNA 1000 Nano kit (Agilent Technologies, Santa Clara, CA). Ribosomal RNA depletion and library construction were performed using the RiboZero Plus kit and TruSeq stranded mRNA library preparation kit (Illumina ...
-
bioRxiv - Physiology 2022Quote: ... Basal oxygen consumption rate and mitochondrial Oxidative Phosphorylation System (OXPHOS) parameters were measured according to manufacturer’s instructions of the Mito Stress test Kit (Seahorse Bioscience, Agilent Technologies, CA, USA). Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... RNA quality was determined by spectrophotometry at 260 nm and 280 nm and by fluorescence capillary assay (RNA 6000 Pico kit, Agilent Technologies, Santa Clara, Ca). RNA integrity numbers ranged from 2.3 to 6.2.
-
bioRxiv - Physiology 2022Quote: ... and concentration prior to library generation with the Agilent RNA Pico 6000 kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA) ...
-
bioRxiv - Neuroscience 2022Quote: ... The fragment distribution of each library was assessed using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies; Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA HS kit (ThermoScientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8×60K V2 Arrays (SurePrint G3 Human Gene Expression 8×60K v2 Microarray Kit ...
-
bioRxiv - Molecular Biology 2022Quote: The reverse transcription reaction was performed on 0.4 µg of RNA template and cDNA was synthesized using an AffinityScript QPCR cDNA Synthesis Kit as instructed by the manufacturer (Agilent Technologies, Les Ulis, France). Quantitative PCR was performed using optimized TaqMan® gene expression assays (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... of the cDNA was assessed on an Agilent Technologies Bioanalyzer 2100 using an Agilent DNA 1000 kit according to the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA, USA).
-
bioRxiv - Neuroscience 2022Quote: ... Quantity and quality of all RNA samples were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). Only samples with an RNA Integrity Number (RIN ...
-
bioRxiv - Biochemistry 2024Quote: A genetic library was constructed based on the metX and metY genes with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA), adjusted to produce an average of 4 nonsynonymous mutations per gene ...
-
bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
-
bioRxiv - Microbiology 2024Quote: ... The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA, USA, 5067-1511). High-Quality RNA samples with RIN number > 7.0 were used to construct the sequencing library ...
-
bioRxiv - Cell Biology 2023Quote: ... Dephosphorylated and denatured total RNA was labeled with cyanine 3-pCp and subsequently hybridized to the Agilent Mouse microRNA Microarray Release 15.0 using the microRNA Complete Labeling and Hyb Kit (Agilent Technologies, Inc., Santa Clara CA). Following hybridization for 20 h ...
-
bioRxiv - Cell Biology 2022Quote: ... The quality of the cDNA product was checked using an Agilent High Sensitivity DNA Kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) to ensure the complete removal of contaminants ...
-
bioRxiv - Biochemistry 2023Quote: ... The cDNA of Affilin variant Af2p was used as template for error-prone PCR employing the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA). The error rate was set to 10-14 mutations per kbp and the resulting theoretical library size was calculated to contain 6·1010 independent variants ...
-
bioRxiv - Cell Biology 2023Quote: ... The size distribution and concentration of the libraries were assessed on Tapestation with a DNA High Sensitivity kit (# 5067-4626, Agilent Technologies, Santa Clara, CA). Paired end 38 bp sequences were generated from samples on a NextSeq 500/550 (Illumina ...