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Citations for Agilent :
801 - 850 of 1488 citations for Mouse RTF1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Bindings were revealed by a HRP-conjugated anti-mouse/rabbit IgG antibodies (Dako) and ECL (Advansta ...
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bioRxiv - Molecular Biology 2019Quote: ... Secondary antibodies used in this study: anti-mouse-HRP 1:1000 (Dako P0260), anti-rabbit-HRP 1:1000 (Dako P0448 ...
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bioRxiv - Molecular Biology 2022Quote: ... the mouse tissue sections were incubated with Target Retrieval Solution (Dako, Glostrup, Denmark) at 98 °C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... USA) and the secondary antibody rabbit anti-mouse-FITC (1:50, #F0232, Dako) as previously described [17] ...
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bioRxiv - Cancer Biology 2022Quote: ... The NET panel included six antibodies: Chromogranin A (DAK-A3, Mouse monoclonal, Dako) at 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies against Ki67 (1:50; Code No. M7249, Dako, Glostrup, Denmark) and BrdU (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies, Glostrup, Denmark) primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... the blots were incubated with anti-mouse (P0447, 1:1000 dilution, DAKO, Denmark) or anti-rabbit (P0448 ...
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies followed by an incubation with ENVISION Flex+ Mouse Linker (DAKO, 15’) for signal amplification ...
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bioRxiv - Physiology 2022Quote: ... We used a Mouse Gene Expression 4 × 44K Microarray chip (G4846A, Agilent Technologies), which can examine 23,215 genes as described [30] ...
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bioRxiv - Immunology 2023Quote: Microarray analysis was performed using SurePrint G3 Mouse GE 8×60K slides (Agilent) and Partek Genomics Suite version 6.6 ...
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bioRxiv - Neuroscience 2023Quote: ... a mouse monoclonal anti-CD68 antibody (clone KP1, 1:50, Dako, Cat# M0814), a rabbit monoclonal anti-Aβ N-terminus (clone D54D2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were then incubated with mouse anti-Ki67 (Dako, M724801-8, 1:200) or rabbit anti-Muc2 antibody (NBP1-31231 ...
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bioRxiv - Cancer Biology 2023Quote: ... Alexa Fluor 488 goat anti-mouse IgG (rabbit polyclonal, P0449: Dako, Glostrup, Denmark), and Alexa Fluor 594 goat anti-rabbit IgG (Dako).
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with polyclonal goat anti-mouse FITC (DAKO F0479 1:20). Finally ...
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bioRxiv - Immunology 2023Quote: ... antirabbit or anti-mouse HRP-conjugated antibodies (1:2000; Dako, Carpinteria, CA, USA) were used for detection of the primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Exome capture was performed using the SureSelectXT Mouse All Exon System (Agilent Technologies). Mapping of WES FASTQ reads to the mouse genome (GRCm38 ...
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bioRxiv - Cell Biology 2023Quote: ... Whole exome sequencing (WES) was performed using the Mouse All Exon kit (Agilent) for target capture followed by next-generation sequencing by Psomagen ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Pathology 2024Quote: ... the sections were stained with EnVision anti-mouse or rabbit immunoglobulin (Dako/Agilent), or anti-sheep secondary antibodies (Jackson ImmunoResearch Inc ...
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bioRxiv - Pathology 2024Quote: ... the sections were stained with EnVision anti-mouse or rabbit immunoglobulin (Dako/Agilent), or anti-sheep secondary antibodies (Jackson ImmunoResearch Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-mouse IgG conjugated with horseradish peroxidase (HRP) (Dako, Carpinteria, CA, USA) for immunohistochemical staining.
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bioRxiv - Biophysics 2023Quote: ... The secondary antibodies used were HRP-conjugated anti-mouse or anti-rabbit (Dako). Signals were imaged using a Fujifilm LAS-3000 system and quantified with ImageJ software.
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... variant hLPYK proteins were created by mutating the coding region of the pLC11 plasmid (originally a kind gift from Dr. Andrea Mattevi 28) using QuikChange (Stratagene) and primers designed to individually obtain all 19 substitutions at each position ...
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... amino acids Lys345 Lys346 to Gln 345 Gln 346) was developed by site-directed mutagenesis of the pGEX6p2-Cx43 CT plasmid (Agilent technologies ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... All GFP-Parkin variants were generated using PCR mutagenesis on the GFP-Parkin WT plasmid (addgene#45875) according to the manufacturer’s protocol (Agilent Technologies). Constructs were verified by Sanger sequencing.
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bioRxiv - Bioengineering 2019Quote: Variant CvFAPG462V was generated by site-directed mutagenesis of the wild-type construct in pETM11 using the QuikChange whole plasmid synthesis protocol (Stratagene) with CloneAmp HiFi PCR premix (Clontech) ...
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bioRxiv - Molecular Biology 2019Quote: ... and A110C mutations were introduced into the HHT2 gene of the pRM200 plasmid (CEN4/ARS1/TRP1/HHT2-HHF2) (Mann and Grunstein, 1992) by site directed mutagenesis (QuikChange, Agilent). The WT or mutagenized pRM200 plasmids were then introduced into the RMY102 strain by standard yeast transformation procedures ...
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bioRxiv - Neuroscience 2019Quote: ... the mouse cDNA sequence for Ntrk2 (GenBank accession number X17647, nucleotides 188-722) was amplified by PCR and cloned into pBluscript II KS (-) plasmid (Stratagene). Antisense probes were synthesized with T3 RNA polymerase (Promega #2083 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
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bioRxiv - Biophysics 2021Quote: The triplex-forming regions were incorporated into the parent plasmids by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene) following the manufacturer’s protocol ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The purified PCR products were subsequently used as megaprimers to generate the library of mutants in the plasmid pZE-ProQ-3×FLAG by following GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... were created by point mutations on the pcDNA3.1-Cyb5r3 (WT) plasmid using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521). Mutagenesis primers were designed using Agilent’s online program ...
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bioRxiv - Microbiology 2020Quote: Huh7.5 cells were seeded 16h prior to transfection with pEGFP-N3-VSV-Gts and p7-encoding plasmid using GeneJammer transfection reagent (Agilent). Medium was changed 4h post-transfection and cells were incubated overnight at 40°C ...
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bioRxiv - Microbiology 2020Quote: ... was generated by introducing mutations in the 3’ splice site of the pPOLI-WSN-M plasmid using QuikChange II site-directed mutagenesis protocol (Agilent). pPolI-WSN-M-M2SMRtoAAA (Mut1) ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Microbiology 2020Quote: ... ftsL30-121 and ftsW were introduced into some plasmids by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies).
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... Correspondent seed-sequence mutated Dual-pMIR-report plasmids were obtained using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies). Low-passage COS7 cells were grown in DMEM (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... MinD and ClpX mutant proteins were constructed by site-directed mutagenesis of plasmids containing minD or clpX using the QuickChange II XL Site-directed mutagenesis kit (Agilent) and confirmed by sequencing prior to purification ...
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...