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Citations for Agilent :
801 - 850 of 1533 citations for Methyl 2 Amino 2 benzyl 3 phenylpropanoate Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Bioengineering 2023Quote: ... on a Shimadzu LC2030C Plus 2 machine equipped with a Poroshell 120EC-C18 column (250 x 4.6 mm, Agilent) and a UV/vis detector ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed with 0.1% Triton X-100 in PBS and the slides were mounted in fluorescence mounting medium (S302380-2, Agilent). Imaging was performed with Cell Observer Spinning Disk Confocal Microscope using the ZEN software ...
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bioRxiv - Cancer Biology 2023Quote: ... All libraries were sequenced (2×100 bp) using Agilent SureSelect RNA XTHS2 All Exon V8 (Agilent, cat. no. G9991A) according to the standard Agilent protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions contained 10 µL of 2× Brilliant III Ultra-Fast SYBR green QPCR master mix (catalog no. 600882; Agilent), 2 µL each of 2 µM PCR primers (see Table S1 for used primers) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
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bioRxiv - Genetics 2023Quote: ... resulting from Cas12a based trans-cleavage were measured every 2.5 min using a BioTek Synergy 2 microplate reader (Agilent). In a diagnostic specificity assay using field samples ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Microbiology 2024Quote: ... cultures were transferred to 21 x14.5 x 5.5 cm3 plastic trays on ice and crosslinked three times at 0.75 J/cm-2 using a UV Stratalinker 2400 (Stratagene) to induce covalent bonds between protein and RNA ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quality was assessed by NanoDrop ND-1000 (260/280 >2; ThermoScientific) and by RNA ScreenTape (RIN ≥7; Agilent). Samples were then sent to the TCAG Microarray Facility at Sick Kids Hospital (Toronto ...
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bioRxiv - Immunology 2024Quote: ... and antigen retrieval performed at 96°C for one hour using pH9 Dako Target Retrieval Solution (S236784-2, Agilent). Sections were stained for CD31 and either collagen I ...
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bioRxiv - Microbiology 2024Quote: ... 2008) medium at 30 °C in a 24-well microtiter plate using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent). Each experiment was carried out in at least three biological replicates with four technical replicates each.
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bioRxiv - Biophysics 2024Quote: ... in xylene and deposited with a 12 μm inter-fiber spacing on top of large diameter (2 μm) support fibers prepared from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Biochemistry 2024Quote: All experiments used 1.5 μL packed bed volume Exp 2 bolt cartridge trapping columns (Optimize Technologies) packed with PLRP (Agilent) solid phase and 150 x 0.3 mm analytical columns packed with 2.6 μm Kinetex core shell particles coated with polar capped C18 (Phenomenex) ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with this media for 4 hours before plating with the assay media (composed of XF Base Medium Minimal DMEM supplemented with 2 mM glucose and 0.5 mM L-carnitine; Agilent) in the XF96 well plate (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... for 2 h and analyzed with an optical density plate reader (plate reader Biotek Epoch2, Agilent Technologies, California, USA) modified from a published method (Oburger et al. ...
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bioRxiv - Neuroscience 2024Quote: ... A permeabilization step for 10 min with 0.1% TritonX-100/PBS was performed before incubation for 1-2 h with serum-free protein block (X0909; Dako). For some targets ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed 2 x 5 min in PBS-T and incubated with streptavidin–horseradish peroxidase complex (DakoCytomation). Following 2 x 5 min washes in PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... dehydrating ethanol series and tissue clearing prior to coverslipping slides with mounting medium (S302380-2, Dako North America Inc.). For cryosectioning ...
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bioRxiv - Physiology 2024Quote: ... the flies were cold anesthetized and collected into an amber glass 2 mL vial (Agilent, P/N 5190-9590) fitted with a 150 µL glass insert (Agilent ...
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bioRxiv - Genetics 2024Quote: ... of approximately 0.1 in 200μl of fresh -HUT media with or without Dox and grown in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent) shaking continuously for up to 25 hrs at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... Amino acid substitutions and deletions were introduced by site-directed mutagenesis using the QuikChange Kit (Agilent).
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bioRxiv - Biophysics 2020Quote: ... Amino acids were then converted into respective fluorescent derivatives using o-phthalaldehyde (OPA) (Agilent #5061-3335). Briefly ...
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bioRxiv - Physiology 2023Quote: ... amino acids and nicotinamide adenine dinucleotide reduced (NADH) and oxidized (NAD+) forms) was created from Agilent METLIN PCDL for identification within the samples ...
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bioRxiv - Cell Biology 2023Quote: ... Amino acid substitutions and deletions were introduced by site-directed mutagenesis using the QuikChange Kit (Agilent). Sequences of the primers used are listed in the Supplementary Table S7.
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bioRxiv - Plant Biology 2023Quote: The AtSHMT1 (AT4G37930) amino acid substitutions were generated using a site-directed mutagenesis kit protocol (Stratagene). The primer-SD was used for Ser190 Leu ...
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bioRxiv - Microbiology 2024Quote: ... Single amino acid mutants were created using the QuikChange II XL site directed mutagenesis kit (Agilent). Primers were designed to introduce mutations at Met68 ...
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bioRxiv - Microbiology 2024Quote: ... Amino acid substitutions and deletions were introduced with the QuickChange II Site-Directed mutagenesis kit (Stratagene), according to the manufacturer’s directions.
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bioRxiv - Plant Biology 2024Quote: ... dissolved in 15 µl pyridine and derivatized with 30 µl N-methyl-N-(trimethylsilyl)trifluoracetamid (MSTFA) before being analyzed by GC-MS (Agilent 7890B GC-Agilent 5977N-MSD) as previously described (Berghoff et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Cell Biology 2021Quote: RNAi-resistant EGFP-ORP5A and EGFP-ORP5B were generated by introducing 4 silent point mutations in the region targeted by the 2 siRNA oligos (#10 and #11) by site-directed mutagenesis (Quickchange II-XL, Stratagene) and the following primers:
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bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.
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bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...