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Citations for Agilent :
801 - 850 of 1054 citations for GM CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by a 30 min incubation at room temperature with the secondary antibody (Envision mouse and rabbit, respectively, Dako) in an autostainer (Dako) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were washed again and an alkaline-phosphate-conjugated goat anti mouse antibody was added (DAKO, 1:2000). Another washing step with TBST and B3 buffer (100 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of primary antibodies (pan-Cytokeratin and α-SMA) was performed by using EnVision-mouse HRP (Dako, Hamburg, Germany) for 45 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Gene expression analysis was conducted using Agilent Whole Mouse Genome 4×44 multiplex format oligo arrays (Agilent Technologies 014868) following the Agilent 1-color microarray-based gene expression analysis protocol ...
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bioRxiv - Microbiology 2021Quote: ... Bound monoclonal antibodies were detected with goat anti-mouse immunoglobulin (Ig) conjugated with horse radish peroxidase (HRP, Dako, Denmark). The reaction was developed with TMB one (Kementec Solutions A/S ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with a HRP-labeled polymer coupled to a secondary anti-mouse antibody (Flex® system, Dako) for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies and dilutions used were as follows: anti-Mouse Immunoglobulins/FITC (1:400, F0479, Dako, Carpinteria, CA, USA) and anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed again and incubated for 30 min at RT with Envision+ HRP labelled polymer anti-mouse (DAKO). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... then followed by immunoblotting for ≤ 1 h with secondary antibodies: 1:5,000 anti-mouse linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies were followed by respective HRP-conjugated anti-mouse or anti-rabbit secondary antibody (1: 2000; Dako). Detection of peroxidase activity was performed by using the Amersham ECL western blot detection kit (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolated mouse cardiomyocytes were seeded at 2K per well in the Seahorse XF96 cell culture microplates (Agilent Technologies, 102416100) pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The bisulfite-converted library was PCR amplified and indexed using the SureSelect XT Mouse Methyl-Seq Kit (Agilent, #G9651A). Prepared libraries were run on an Illumina sequencing platform using a NextSeq High 150 bp paired-end mode.
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bioRxiv - Cell Biology 2019Quote: ... washed in PBS before saturation with 0.6 μg/mL mouse IgG2 diluted in Dako Antibody Reducing Background buffer (Dako) for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were subsequently washed three times for 10 minutes in PBS with 0.05% Tween20 and incubated with horseradish peroxidase (HRP) conjugated anti-rabbit or anti-mouse secondary antibody (Dako) in 5% SMP in PBS ...
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bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark), as secondary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies). The enzymatic reaction was conducted with 1-Step Ultra TMB-ELISA (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... they were then washed three time in PBS containing 0.1% tween 20 and incubated 1h with a rabbit anti-mouse antibody conjugated with horseradish peroxidase (HRP) (Dako). The activity of HRP was revealed by enhanced chemiluminescence (Perkin-Elmer).
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bioRxiv - Neuroscience 2021Quote: ... followed by washing and detection of bound Adu with a rabbit anti-mouse horse radish peroxidase conjugated antibody (Dako) and 3,3′,5,5′-tetramethylbenzidine substrate ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA obtained from FACS-sorted E7.5 mouse embryonic populations was purified with the Absolutely RNA nanoprep kit (Agilent) and quantified as above ...
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bioRxiv - Bioengineering 2021Quote: ... Serotec) mouse monoclonal antibodies, or Aβ40 (1:200, Covance), Iba-1 (1:200, Wako) and GFAP (1:1000, DAKO) rabbit polyclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated at 4°C for 30 minutes with an unconjugated antibody for IAV NP (HB65, 8,0 μg/ml) followed by a PE-conjugated goat anti-mouse secondary antibody (DAKO) and measured on a flow cytometer (BD FACSLyric).
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bioRxiv - Neuroscience 2022Quote: ... For PrP deposition anti-PrP monoclonal antibodies ICSM3525,26 were used in conjunction with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary anti-mouse-HRP (Santacruz sc-516102 Lot#c1419) was diluted 1: 10,000 or anti-rabbit-HRP (Dako P0448) 1:1000 in blocking buffer and incubated for 1hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by washing in TBST (0.1%) and a 1 h incubation in secondary antibody (goat a-mouse-HRP: 1:10000, P0447, Dako). After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... immunohistochemical staining was performed using an antibody directed against the mouse endothelial cell surface molecule CD31 (JC70A, DAKO, Glostrup, Denmark). 10 μm-thick cryosections were fixed with 4% PFA solution for 20 min at room temperature ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... then washed and incubated with Labelled polymer HRP-anti-Rabbit and Labelled polymer HRP-anti-mouse secondary antibody (Dako, Denmark) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... then probed for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG secondary antibodies 1:500 in PBS (Dako, Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... mouse MLE12 cells were seeded at a concentration of 20,000 cells/well on XFp cell plates (Seahorse Bioscience, 103057-100) 24 hours prior to the initiation of studies ...
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bioRxiv - Cell Biology 2019Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: The Bub1 K795R allele was generated by a single-base substitution in full-length mouse Bub1 cDNA cloned into pcDNA3/Myc using site-directed mutagenesis (Stratagene), then subcloned into a pShuttleCMV vector as a BglII-NotI digest ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated with primary or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA) or negative isotype control rabbit IgG (cat ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with secondary antibody polyclonal rabbit anti-mouse HRP-conjugate (cat.P0260, Dako, Agilent Technologies, Santa Clara, CA, USA) diluted 1:100 in PBS 1× for 1h at room temperature (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98, Proteintech, 1:1000; rabbit anti-FLAG polyclonal, Sigma, 1:2000; mouse anti-FLAG monoclonal M2, Stratagene 1:2000 ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...