Labshake search
Citations for Agilent :
801 - 850 of 897 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... slides were rinsed three times in milli-Q water before submerging into bluing buffer (Bluing Solution, Cat No. CS702, Dako). Slides were washed once in milli-Q water ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by a double wash with PBS and with distilled water and mounting on slides using a mounting solution (Dako).
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed at 95-98°C for 30 min using 1X of Target Retrieval Solution pH9 (S236784-2, Agilent). The tissue slides were cooled down for 20 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
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bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
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bioRxiv - Cell Biology 2024Quote: ... Staining solution was once again rinsed and replaced with PBS and cell count was analyzed using a Biotek Cytation1 (Agilent) measuring puncta count at Ex.377 ...
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bioRxiv - Plant Biology 2020Quote: ... sucked 500 μl solution from the tube to detect the concentrations of remaining nitrate using High Performance Capillary Electrophoresis System (Agilent Technologies). Then measured the root fresh weights and calculated the nitrate uptake speeds (NO3− (μmol)/root fresh weight (g)/h) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Cell Biology 2022Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 37°C in a non-Co2 incubator and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Genomics 2020Quote: ... gDNA before SRE treatment was diluted 5-fold and the undiluted solution after SRE treatment was used according to the protocol using TapeStation 2200 (Agilent Technologie) and Genomic DNA ScreenTape (Agilent Technologie).
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bioRxiv - Pathology 2020Quote: ... The quantity and quality of the RNA solution was assessed using a Qbit machine and an Agilent 2100 Bioanalyzer (Agilent Technologies) before library construction and sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... The release process of photo-uncaged lifitegrast from the contact lens to its bathing solution was monitored using UV-vis absorption spectroscopy recorded by an Agilent 8453 UV–vis spectrophotometer (Agilent Technologies).
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The mass analyzer was calibrated between m/z 118 and 2721 using an Agilent ESI-L low concentration tuning mix solution (Agilent, USA) at a resolution of 20 000 giving a mass accuracy below 2 ppm ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). After processing of raw data as previously described24 ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
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bioRxiv - Plant Biology 2022Quote: ... 100μL of hybridization solution was dispensed into the gasket slide and assembled to the Agilent SurePrint HD Arabidopsis GE 4X44K Microarrays (Agilent®). Slides were incubated for 17 h at 65°C in an Agilent hybridization oven ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Formalin-fixed samples tissue microarrays of EwS-samples and normal tissues were stained for GPR64 after antigen retrieval using Target Retrieval Solution (Fa.Agilent Technologies, S1699) with anti-GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Neuroscience 2021Quote: Paraffin-embedded sections were deparaffinized and autoclaved at 121°C for 20 min in Target Retrieval Solution (Dako Japan Inc., S1700). Frozen sections were briefly washed in PBS and incubated with 0.5 % SDS (v/v ...
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bioRxiv - Pathology 2022Quote: ... paraffin embedded (FFPE) tissue sections were wax removed with Xylene and incubated in Target retrieval solution pH 9.0 (Dako, Glostrup, Denmark) for 10 min at room temperature prior to incubating for 15min at 110°C ...
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The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligamentsbioRxiv - Molecular Biology 2020Quote: ... 30μg of the soluble fraction was subjected to an in-solution digestion on 10 μl Strataclean™ resin (Agilent Genomics, UK) followed by reduction and alkylation in 3 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2020Quote: ... The solution was filtered and then injected into LC-MS system (Agilent 6538 UHD-QTOF equipped with Agilent 1290 infinity UPLC). Upon extracting the chromatograms based on the reported m/z ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed for 15 min in the autoclave (250°F) using 1x Target antigen retrieval solution (Dako; S169984-2). All antibody steps were performed as described above ...
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bioRxiv - Immunology 2021Quote: ... Slides were then rinsed with double distilled water (ddH2O) and boiled in 1X Dako pH9 antigen retrieval solution (Agilent, S236784-2) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... The sulfo-SANPAH solution was placed on gels and activated using 1 Joule of 254 nm UV light (Stratagene UV crosslinker). Afterwards ...
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bioRxiv - Cancer Biology 2022Quote: ... dried metabolites were resuspended in 50 μL of a 1:1 DMSO to water solution and analyzed by LC-MS using a 1290 Infinity II liquid chromatography (Agilent Technologies) with a thermal autosampler set at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin sections were rehydrated through graded alcohols and antigen retrieval was performed using Target Retrieval Solution (Dako, Carpinteria, CA, United States), pH 6.0 ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on 4-μm thick formalin-fixed paraffin-embedded (FFPE) whole tissue serial sections following antigen retrieval (Target Retrieval Solution, pH 6.1; Dako, Carpinteria, CA). Immunostains performed included Connexin 26 (Cx26 ...
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bioRxiv - Biophysics 2023Quote: ... BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer, Agilent Technologies). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed by heating slides in a pressure cooker for 45 minutes with Target Antigen Retrieval Solution (Dako, S1699). Sections were blocked with 10% goat serum for 30 minutes and primary antibodies were incubated at 4°C overnight ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Bioengineering 2024Quote: ... The pellets from centrifugation were dried under vacuum and re-dissolved using 5% acetic acid aqueous solution for purification by HPLC (1260 Infinity, Agilent Technologies) equipped with a C8 column (Zorbax 300SB-C8 ...
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bioRxiv - Biochemistry 2024Quote: ... A reference mass correction solution was continually infused at a flow rate of 0.5 mL/min via an external isocratic pump (Agilent, Cheadle, UK) for constant mass correction (see preparation of reference mass correction solution) ...
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bioRxiv - Bioengineering 2024Quote: ... then gradually increase the pH by adding NaOH (aq.) to the peptide solution and monitor the turbidity changes by spectrophotometer at the wavelength of 600 nm (Agilent carry3500). When a turbidity appeared ...
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bioRxiv - Physiology 2024Quote: ... the cell pellet was resuspended in 500ul of warm MAS buffer solution [58] + 1nM Plasma Membrane Permeabilizer (Agilent Seahorse XF PMP) to permeabilize the cells ...
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bioRxiv - Immunology 2024Quote: ... the formalin-fixed paraffin-embedded human PDAC tissue sections were deparaffinized by Xylene and gradient alcohol solutions and stained with hematoxylin (S3301, Dako, US) for 1 min ...