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751 - 800 of 2764 citations for Recombinant HIV 1 gp120 His tagged since 2019

• A reference single-cell map of freshly dissociated human synovium in inflammatory arthritis with an optimized dissociation protocol for prospective synovial biopsy collection

  Sam G. Edalat, et al., bioRxiv - Genomics 2023

  Quote: ... CD20, Ventana Roche L26, prediluted; CD3, Leica LN10, 1:500; CD68, DAKO A/S PG-M1, 1:50; CD138, DAKO A/S ...
• Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses

  Jinyang Wang, et al., bioRxiv - Biochemistry 2023

  Quote: ... shRNAs against mouse ACOT12 (#5, #6 and #7) and mouse ACOT8 (#1 and #2) were also constructed in pAdEasy-1 (Stratagene) for adenovirus packaging based on The AdEasyTM Technology (He et al. ...
• Defining transcription factor nucleosome binding with Pioneer-seq

  Maria Tsompana, et al., bioRxiv - Molecular Biology 2023

  Quote: ... which was amplified using Herculase II Fusion DNA polymerase in 100-µl reaction mixtures (1× Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
• Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinoma

  Chad VanSant-Webb, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The organic samples were resuspended in 200 µL of 2:1:1 isopropyl alcohol:acetonitrile:water and transferred to amber glass mass spectrometry vials (Agilent 5182-0716) containing a glass insert ...
• Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinoma

  Chad VanSant-Webb, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The organic samples were resuspended in 35x the packed cell volume using 2:1:1 isopropyl alcohol:acetonitrile:water and transferred to amber glass mass spectrometry vials (Agilent, 5182-0716) containing a glass insert ...
• Altered projection-specific synaptic remodeling and its modification by oxytocin in an idiopathic autism marmoset model

  Jun Noguchi, et al., bioRxiv - Neuroscience 2023

  Quote: ... free-floating brain slices were incubated in a solution of anti-Iba1 antibody (1:2000; Fuji film-Wako) or anti-GFAP antibody (1:10,000; Agilent-Dako) at 4°C overnight ...
• Calcitonin gene-related peptide and intermedin induce phosphorylation of p44/42 MAPK in primary human lymphatic endothelial cells in vitro

  Shirin R. Hasan, et al., bioRxiv - Cell Biology 2024

  Quote: ... goat horseradish peroxidase (HRP) anti-mouse IgG (#P0447; 1:1000) and anti-rabbit IgG (#P0448; 1:1000) were from Dako.
• Characterizing neuroinvasion and neuropathology of SARS-CoV-2 by using AC70 human ACE2 transgenic mice

  Jason C. Hsu, et al., bioRxiv - Pathology 2024

  Quote: ... the above IHC protocol was modified such that the anti-SARS-CoV-2 S primary antibody was at 1:1000 dilution (1 µg/mL) in background-reducing antibody diluent (Dako, S302283-2 ...
• Crystal Structure of the Werner’s Syndrome Helicase

  Joseph A. Newman, et al., bioRxiv - Biochemistry 2020

  Quote: ... and secondary rabbit (Dako, P0448, 1/1000 dilution), mouse anti-IgG-HRP (Dako ...
• Engraftment of allotransplantated tumour cells in adult rag2 mutant Xenopus tropicalis

  Dieter Tulkens, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and anti-PCNA antibody (1:1000, PC10, Dako). Following secondary antibodies (all 1:500 ...
• Non-canonical Staphylococcus aureus pathogenicity island repression

  Laura Miguel-Romero, et al., bioRxiv - Molecular Biology 2021

  Quote: ... and 1 µl of PfuTurbo DNA polymerase (Agilent) for amplification in a final volume of 50 µl ...
• Direct reprogramming of human fibroblasts into insulin-producing cells by transcription factors

  Marta Fontcuberta-PiSunyer, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Primary antibodies used were: Insulin (DAKO, 1:400), C-peptide (Abcam ...
• Plasmodium falciparum GBP2 is a telomere-associated protein that binds to G-quadruplex DNA and RNA

  James Edwards-Smallbone, et al., bioRxiv - Microbiology 2021

  Quote: ... 1:1500 goat anti-mouse IgG-HRP (Dako). Coomassie staining of recombinant protein after gel electrophoresis was performed by addition of 0.1% Brilliant blue R-250 for 20 mins (Fisher) ...
• Characterization of TR-107, a Novel Chemical Activator of the Human Mitochondrial Protease ClpP

  Emily M. J. Fennell, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... 1 μM Rotenone/Antimycin A (Agilent, 103015-100) were added at indicated timepoints ...
• Preterm birth alters the maturation of the GABAergic system in the human prefrontal cortex

  Helene Lacaille, et al., bioRxiv - Neuroscience 2021

  Quote: ... GFAP (1:1000, Agilent Dako, Glostrup, Denmark, Z0334), Neuropeptide Y (1:500 ...
• Preterm birth alters the maturation of the GABAergic system in the human prefrontal cortex

  Helene Lacaille, et al., bioRxiv - Neuroscience 2021

  Quote: ... GFAP (1:1000, Agilent Dako, Glostrup, Denmark, Z0334), Neuropeptide Y (1:500 ...
• Hotair and Malat1 Long Noncoding RNAs regulate Bdnf Expression and Oligodendrocyte Precursor Cells Differentiation

  Fatemeh Khani-Habibabadi, et al., bioRxiv - Neuroscience 2021

  Quote: ... Anti-GFAP antibody (1:300, Dako Z0334, USA), and Anti-Aba1 antibody (1:200 ...
• Multiple links between 5-methylcytosine content of mRNA and translation

  Ulrike Schumann, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-mouse-HRP (1:5,000; Agilent Dako P0260), anti-mouse-IR-Dye800 (1:10,000 ...
• Multiple links between 5-methylcytosine content of mRNA and translation

  Ulrike Schumann, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-mouse-HRP (1:5,000; Agilent Dako P0260), anti-mouse-IR-Dye800 (1:10,000 ...
• The antimicrobial peptide cathelicidin is critical for the development of Th17 responses in severe inflammatory disease

  Katie J Smith, et al., bioRxiv - Immunology 2022

  Quote: ... and CD31 (JC70A, Dako M0823, dilution 1:100).
• KAT2-mediated acetylation switches the mode of PALB2 chromatin association to safeguard genome integrity

  Marjorie Fournier, et al., bioRxiv - Cell Biology 2020

  Quote: ... goat anti-rabbit (Dako P0448, WB: 1/1000).
• A Novel Tmem119-tdTomato Reporter Mouse Model for Studying Microglia in the Central Nervous System

  Chunsheng Ruan, et al., bioRxiv - Immunology 2019

  Quote: ... rabbit anti-GFAP (1:500; #ZO334; Dako, USA), rabbit anti-Olig2 (1:500;#AB9610 ...
• Patient-matched analysis identifies deregulated networks in prostate cancer to guide personalized therapeutic intervention

  Akinchan Kumar, et al., bioRxiv - Genomics 2019

  Quote: ... Androgen Receptor (Dako, M3562, 1/200, pH 9). Slides were digitalized using a Hamamatsu NanoZoomer slide scanner (Japan ...
• Regulation of HDAC2-PDX1 by RNF125 defines pancreatic cancer development

  Erez Hasnis, et al., bioRxiv - Cancer Biology 2020

  Quote: ... pan-keratin (Dako; #M3515, IHC dilution 1:100), Ki67(Abcam ...
• Axon-like protrusions promote small cell lung cancer migration and metastasis

  Dian Yang, et al., bioRxiv - Cancer Biology 2019

  Quote: ... TAU (1:1000, Dako A0024, Santa Clara, CA), and MAP2 (1:500 ...
• DNA end-resection in highly accessible chromatin produces a toxic break

  Jeroen van den Berg, et al., bioRxiv - Cell Biology 2019

  Quote: ... and goat anti-mouse (P447 DAKO, 1:2000). Secondary antibodies used for immunofluorescence and FACS analysis were anti-rabbit Alexa 488 (A11008 Molecular probes ...
• A unique cerebellar pattern of microglia activation in a mouse model of encephalopathy of prematurity

  Luisa Klein, et al., bioRxiv - Neuroscience 2021

  Quote: ... or polyclonal rabbit anti-mouse (1:5000; Dako) antibodies ...
• Microbiota-derived short chain fatty acids promote Aβ plaque deposition

  Alessio Vittorio Colombo, et al., bioRxiv - Neuroscience 2020

  Quote: ... or anti-GFAP (1:200, DakoCytomation Z 0334), mouse anti-6E10 antibody (1:200 ...
• Impact of endotoxins in gelatine hydrogels on chondrogenic differentiation and inflammatory cytokine secretion in vitro

  Wilhelmina M.G.A.C. Groen, et al., bioRxiv - Bioengineering 2020

  Quote: ... Mouse monoclonal IgG1 antibody (DAKO, X0931, 1:100) was used as a negative control ...
• A human brain vascular atlas reveals diverse cell mediators of Alzheimer’s disease risk

  Andrew C. Yang, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit polyclonal anti-VWF (1:100, GA527, Dako), rabbit polyclonal anti-SLC39A10 (1:100 ...
• Mapping of the fibrinogen-binding site on the staphylocoagulase C-terminal repeat region

  Ashoka A. Maddur, et al., bioRxiv - Biochemistry 2021

  Quote: ... and 1 unit of high-fidelity polymerase (Stratagene). A C-terminal Cys residue for 5-IAF labeling was introduced by site-directed mutagenesis (QuikChange ...
• Rescue of behavioral and electrophysiological phenotypes in a Pitt-Hopkins syndrome mouse model by genetic restoration of Tcf4 expression

  Hyojin Kim, et al., bioRxiv - Neuroscience 2021

  Quote: ... and 1:1000 rabbit anti-GFAP (Dako, Z0334). The following secondary antibodies from Invitrogen (Carlsbad ...
• Cancer cell-specific MHCII expression as a determinant of the immune infiltrate organization and function in the non-small cell lung cancer tumor microenvironment

  Amber M. Johnson, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD8 (clone C8/144B, dilution 1:100, Dako), HLA-DR + DP + DQ (MHCII ...
• Spatial transcriptomics of tumor microenvironment in formalin-fixed paraffin-embedded breast cancer

  Lou Romanens, et al., bioRxiv - Cancer Biology 2020

  Quote: ... CD68 (clone M0876, Dako®, dilution 1: 100) and CD8 (clone ...
• High Sucrose Diets Contribute to Brain Angiopathy with Impaired Glucose Uptake, and Psychosis-related Higher Brain Dysfunctions in Mice

  Shinobu Hirai, et al., bioRxiv - Neuroscience 2021

  Quote: ... and rabbit anti-fibrin (Dako, A0080, 1:500). Thereafter ...
• Acyloxyacyl Hydrolase is a Host Determinant of Gut Microbiome-Mediated Pelvic Pain

  Afrida Rahman-Enyart, et al., bioRxiv - Systems Biology 2021

  Quote: ... at 1:100 in Antibody Dilution Buffer (DAKO) overnight at 4 °C ...
• Aerosol Exposure of Cynomolgus Macaques to SARS-CoV-2 Results in More Severe Pathology than Existing Models

  Sandra L. Bixler, et al., bioRxiv - Microbiology 2021

  Quote: ... rabbit anti-MPO (1:200, A039829-2, Dako Agilent Pathology Solutions ...
• Aerosol Exposure of Cynomolgus Macaques to SARS-CoV-2 Results in More Severe Pathology than Existing Models

  Sandra L. Bixler, et al., bioRxiv - Microbiology 2021

  Quote: ... rabbit anti-CD3 (1:200, A045229-2, Dako Agilent Pathology Solutions ...
• Cardiac Atrial Compartmentalisation Proteomics: A Modified Density Gradient Method to Analyse Endo-lysosomal Proteins

  Thamali Ayagama, et al., bioRxiv - Molecular Biology 2021

  Quote: ... Goat anti-rabbit antibody (1:2500, Dako P0448) was used as the secondary antibody to detect the protein markers of lysosomes ...
• Inter- and intrapopulational heterogeneity of characteristic markers in adult human neural crest-derived stem cells

  Beatrice A. Windmöller, et al., bioRxiv - Cell Biology 2021

  Quote: ... S100 (Rabbit, Z0311, 1:400, Dako Cytomation, Germany), Slug (Rabbit ...
• The Metabesity Factor HMG20A Potentiates Astrocyte Survival and Reactivity Preserving Neuronal Integrity

  Petra I. Lorenzo, et al., bioRxiv - Cell Biology 2021

  Quote: ... Rabbit anti-GFAP (1:200, Dako, Barcelona, Spain), mouse anti-GFAP and rabbit anti-HMG20A (1:1000 ...
• Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration

  Carlos Pérez-González, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti CK20 1:50 (Dako Cat M7019), mouse anti Ki67 1:100 (BD Biosciences Cat BD550609) ...
• Nrf2 modulates the hybrid epithelial/mesenchymal phenotype and Notch signaling during collective cancer migration

  Samuel A Vilchez Mercedes, et al., bioRxiv - Cancer Biology 2021

  Quote: ... E-cadherin (1:50, cat. #M3612, Agilent Dako), ZEB1 (1:100 ...
• Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

  Kevin Christian M. Gulay, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD3 (1:1000; Agilent Technologies, CA, USA, IR503), KDM2B (1:50 ...
• Engineering a 3D hydrogel system to study optic nerve head astrocyte morphology and behavior in response to glaucomatous insult

  Ana N. Strat, et al., bioRxiv - Bioengineering 2022

  Quote: ... or GFAP (rabbit anti-GFAP, 1:100, Dako) overnight ...
• 3D-imaging reveals conserved cerebrospinal fluid drainage via meningeal lymphatic vasculature in mice and humans

  Laurent Jacob, et al., bioRxiv - Neuroscience 2022

  Quote: ... Rabbit anti–human vWF (A0082, Agilent, 1:300). Primary antibodies were detected with the corresponding Alexa Fluor -555 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-Gfap (1:1000, Dako/Agilent, GA524); rat anti-Gfap (1:1000 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-Gfap (1:1000, Dako/Agilent, GA524); rat anti-Gfap (1:1000 ...
• Reactive astrocytes transduce blood-brain barrier dysfunction through a TNFα-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

  Hyosung Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-GFAP (1:300 dilution, Z0334, Dako), mouse anti-GFAP (1:300 dilution ...
• Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly-dT termination signals

  Mathias Girbig, et al., bioRxiv - Biochemistry 2022

  Quote: ... coli XL-1 Blue chemically competent cells (Agilent). The next day ...