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Citations for Agilent :
7901 - 7950 of 8233 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Systems Biology 2023Quote: ... we calibrated the TIMS elution voltages by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Immunology 2024Quote: ... after which live cell imaging analysis was carried out for 30 minutes at a rate of 1 frame per 30 or 40 seconds using BioTek LION Heart XL (Agilent) or TomoStudio (Tomocube ...
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bioRxiv - Cancer Biology 2024Quote: ... These tumor sections were stained at the Histology core (Henry Ford Health) according to the manufacturer’s protocols (Ki-67 IHC MIB-1, Dako Omnis). Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used in this study: rabbit anti-mouse immunoglobulins/HRP (Agilent Cat# P0260, RRID:AB_2636929, 1/10000), goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: MBP-MPS1104-330/WT and MPS1104-330/4A were obtained by over-expression in 1 L culture of BL21CodonPlus(DE3)-RIL cells (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... infected with VSVΔM51-GFP at the indicated MOIs and treated with 0.3-3µM CBL0137 (or media alone) 1 hpi using the Agilent Bravo liquid handling system (Agilent, CA, USA). 24 hpi ...
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bioRxiv - Microbiology 2024Quote: ... The amplified vectors were digested with Dpn1 and 1 µL of each (vector and promoter region insert) were transformed into ultracompetent XL-10 Gold cells (Agilent) for in vivo assembly ...
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bioRxiv - Immunology 2024Quote: ... Serum samples were diluted 1:50 in PBS + 25% FBS and data were acquired with a BioTek Synergy H1 plate reader (Agilent). ELISA data were analyzed with GraphPad Prism (Dotmatics ...
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bioRxiv - Microbiology 2024Quote: ... membranes were immunoblotted for 1 hour with secondary antibodies (1:5,000) anti-mouse or anti-rabbit linked to peroxidase (reference number: P0260 and P0217, respectively, Dako Agilent). Signal for (3xFLAG)TamL was generated using Clarity™ Western ECL substrate chemiluminescence reagent (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... The single and triple-site mutations were introduced into the pcDNA3.1 vector encoding SARS-CoV-2 wildtype or BA.1 Spike using QuickChange site-directed mutagenesis (Agilent 210519) according to the instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
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bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM Seahorse XF L-glutamine solution and 1 mM Seahorse XF pyruvate solution (103577-100, 103579-100 and 103578-100, all from Agilent) and cells were kept in a CO2 free incubator for 45 min prior to starting the Cell Mito Stress Test ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... which was amplified in 15 PCR cycles using Herculase II Fusion DNA polymerase in a 100-μl reaction mixtures (1x Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
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bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 h before being placed into a Seahorse XFe24 Analyzer (Agilent). For OCR and ECAR mitostress test experiments ...
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bioRxiv - Physiology 2024Quote: ... or 32°C (naked mole- rat) and the O2 level was set to 1%O2 using the gas controller (Agilent). After 4 hours of hypoxia ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were incubated for 2 h at 4℃ in a guinea pig anti-insulin antibody (1:200, A0564, Dako), a rabbit anti-somatostatin antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed and re-suspended in HBSS with 2% FBS with 1 μg/ml propidium iodide before acquisition on a Novocyte Quanteon or Penteon (Agilent).
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bioRxiv - Biochemistry 2024Quote: ... the peptide arrays were incubated with a 1:1,000 dilution of an appropriate antibody peroxidase conjugate (anti-rabbit immunoglobulins HRP conjugate, DAKO, P0217) for one hour at 25°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed 5 times with 1X PBST and incubated with horseradish peroxidase conjugated secondary anti-mouse or anti-rabbit IgG (1:5000, Cat # P0161 and P0448, respectively; DAKO) in 1X PBST for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times with TBS/T before HRP-coupled secondary antibodies were applied 1:25000 in TBS and incubated for 2 h at RT (Polyclonal rabbit anti-mouse, Dako P0260 ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002, Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002 ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence was monitored with excitation and emission filters set at 465 and 590 nm during gradual heating (1 K/min) from 25 °C to 95 °C in a MX3005P real-time PCR instrument (Stratagene). Data was evaluated using MXPro3005 software (Stratagene ...
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bioRxiv - Cell Biology 2024Quote: ... A 96-well flat bottom MaxiSorp immuno plate was coated with 1 µg/mL anti-vWF antibody (Dako rabbit polyclonal) in a sodium carbonate/bicarbonate buffer pH 9.5 for 1 h at room temperature prior to blocking with 3% w/v BSA in PBS-T for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells (1×104) of the indicated genotypes were seeded in 96-well Seahorse XFe96 cell culture microplates (Agilent Technologies) for 24 h in eight replicate wells ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
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bioRxiv - Microbiology 2020Quote: The quality of purified libraries was analyzed on a Bioanalyzer High Sensitivity DNA chip (Agilent; see Figure 1 for an example) by following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: 3000 control or p107KO cells or p107KO cells 24 hours post transfection with pCMV6-OCT-HA-eGFP alone or together with p107fl or p107mls were seeded in DMEM (Wisent) containing 10% FBS and 1% penicillin streptomycin on microplates (Agilent Technologies) and treated according to the required experiment ...
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bioRxiv - Immunology 2021Quote: Total IgG from individual donors was purified from ∼1 µL plasma using the AssayMAP Bravo platform (Agilent Technologies, Santa Clara, USA) with Protein G-coupled cartridges as described elsewhere (Larsen et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...