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Citations for Agilent :
7901 - 7950 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Secondary anti-mouse-HRP (Santacruz sc-516102 Lot#c1419) was diluted 1: 10,000 or anti-rabbit-HRP (Dako P0448) 1:1000 in blocking buffer and incubated for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... RNA quality was measured by 1% agarose gel electrophoresis (Figure S3A) and by High Sensitivity (HS) DNA Assay (Agilent) (Figure S3B).
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Synthetic Biology 2023Quote: ... An Agilent 7693A autosampler fitted with a 100 µL gastight syringe was used to inject headspace gas (50 µL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent). Experiments were performed using a 55:1 split ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added with the estimation that OD600 of 1 is approximately equal to 8×108 CFU/ml (Agilent biocalculator). The MOI of 0.01 was administered to E ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum-free Protein Block solution (Agilent, USA) for 45 min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... or 1 µM (OMM1) carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP) and 0.5 µM of a rotenone antimycin A mix (Agilent Technologies). Following the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F or Q157R were treated with doxycycline (1 μg/mL) for 48 hours before UV-crosslinking (400 mJ/cm2 UV Stratalinker 2400, STRATAGENE). RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Developmental Biology 2020Quote: ... then 1 ml of the headspace gas was withdrew and injected into a gas chromatograph (Agilent GC 6890N, CA, USA) fitted with a flame ionization detector and an activated alumina column (Zhang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Size of chromatin fragments was verified on a 1% agarose gel and on a high sensitivity DNA chip in a 2100 bioanalyzer (Agilent) after decrosslinking overnight at 65°C with shaking in elution buffer (100 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription for PCR analysis was performed using 1 μg total RNA and 300 ng random hexamers using Affinity script multiple temperature reverse transcriptase (Agilent). For UTP11 ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2019Quote: ... Immunofluorescence staining was carried out using the following antibodies: a primary rabbit anti-GFAP (resting and reactive astrocytes, 1:500 dilution, Z0334, Dako), and a primary rabbit anti-Iba1 (resting and activated microglia/macrophages ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then diluted 1:11 in ultrapure water before analysis with ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent). Calibration was carried out using standards of Zn ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako) in a dilution of 1:1500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the beads were resuspended in 50 μL Luminex block buffer containing a 1:5,000 dilution of streptavidin-phycoerythrin (Prozyme #PJ31S). The plate was shaken at 1100 RPM for 30 sec and incubated for 30 min at 600 RPM ...
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bioRxiv - Microbiology 2019Quote: UV-Visible spectra were recorded in 1 cm optic path quartz cuvette under aerobic conditions on a Cary 100 UV-Vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Cancer Biology 2019Quote: ... single strand cDNA synthesis and PCR amplification were carried out in a 1-step reaction using the Brilliant II QRT-PCR Master Mix (Agilent) and TaqMan gene expression assays (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: HEK293T cells were seeded in 96-well plates (1×104 cells/well) (Thermoscientific, 136101) and transfected with pNF-κB-Luc (Stratagene) and phRK-TK (Renilla ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Neuroscience 2020Quote: ... IdU) on alternate one-in-ten sections using different anti-BrdU antibodies from different vendors (for BrdU: 1/200, Dako, Glostrup ...
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bioRxiv - Cell Biology 2021Quote: The 3a ORF from SARS CoV-1 (Tor2 strain) was originally obtained from the Institute for Genomic Research and subcloned into pBluescript (Stratagene) prior to transferring to pCAGGS-MCS [17] after PCR amplification with KpnI and XhoI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and retrovirus expressing EGFP in alternating one-in ten sections using different anti-BrdU antibodies from different vendors (for BrdU: mouse primary at 1/100, Dako; CldU ...
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bioRxiv - Molecular Biology 2021Quote: ... by inserting synthesized coding sequence for full length hRpn13 (NM_007002.3) or for residues 1–279 between the BamHI and HindIII restriction sites of pCMV-3Tag-1a (Agilent Technologies, 240295). Unmodified pCMV-3Tag-1a was used as empty vector (EV ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Biochemistry 2021Quote: Rat Kir6.1 and N-terminal FLAG-tagged (DYKDDDDK) SUR2B were first cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses in HEK293 cells according to manufacturer’s instructions63,64 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane produced following transfer was probed with MAb214 primary antibody and then visualized with anti-mouse horseradish peroxidase secondary antibody (1:1000; Dako) for 1 h.
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bioRxiv - Neuroscience 2021Quote: ... A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody at a dilution of 1:7000 (Dako) or 1:2000 (Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections (50 μm thickness) were prepared by a microslicer (DTK-1000, Dosaka EM) and treated with 1 mg/mL pepsin (DAKO) in PBS containing 0.1% Triton X-100 and 0.2 N HCl (Wako ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 2 hours at room temperature with polyclonal guinea pig anti-insulin antibody (1:5, Agilent Technologies). Slides were then washed 3 x 5 minutes with PBS and incubated with the Anti-guinea pig IgG Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...