Labshake search
Citations for Agilent :
7701 - 7750 of 7968 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Quality and quantity of each RNA sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). All RNA samples were stored at −80°C.
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bioRxiv - Genetics 2020Quote: ... the exonic regions and flanking splice junctions of the genome were captured using the Clinical Research Exome kit (Agilent Technologies, Santa Clara, CA). Massively parallel (NextGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The quality of the library was assessed using High Sensitivity D1000 kit on a 2200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA). Sequencing was performed using the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... The mutated variants of AopW1 were generated by inserting mutations in the corresponding primers in combination with the Quick Change Lightening Site Directed Mutagenesis kit (Agilent Technologies, SC, USA). For this purpose ...
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bioRxiv - Plant Biology 2020Quote: ... Site-directed mutagenesis to generate the different H3.1 point mutant constructs was performed using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ADA2b coding sequence was cloned into pETDuet-1 (Millipore ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR of complementary DNA was performed using the DyNAmo HS SYBR Green kit (Thermo-Fisher, Cat # F410L) on an Mx3000P machine (Stratagene, La Jolla, CA) using the primers listed in Supplementary File 3 ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using mid output 150-bp paired-end (PE ...
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bioRxiv - Systems Biology 2019Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (Agilent Technologies, 5067-1513). Lowest RIN was 9.5 ...
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bioRxiv - Plant Biology 2019Quote: ... Mutations found in Bgh51 of Australian isolates were introduced into pYES-Bgh51wt through a QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA).
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bioRxiv - Evolutionary Biology 2019Quote: ... of total cytoplasmic RNA was quantified by an Agilent 2100 Bioanalyzer using an Agilent RNA 6000 Nano Kit (#5067-1511, Agilent, Santa Clara, CA). Samples with a total cytoplasmic RNA average RIN value of 7.5±0.16 were used for RNA-seq library preparation of the nuclear samples ...
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bioRxiv - Genomics 2021Quote: ... and the library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were sequenced on an Illumina HiSeq2500 instrument (Illumina ...
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bioRxiv - Genetics 2021Quote: ... The integrity of RNA was assessed with an RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Biochemistry 2021Quote: ... Single amino acid changes in the RBD (N501Y, E484K, K417N) and combinations thereof were introduced using QuikChange (XL Site-Directed Mutagenesis Kit, Agilent, Santa Clara, USA). Resulting expression plasmids were purified using Nucleobond Xtra maxi ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies, United States) according to the manufacturer’s instructions and the respective parental vector as template ...
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bioRxiv - Cancer Biology 2020Quote: ... The slides were thawed at room temperature for 15 minutes prior to immunohistochemistry staining using Envision+ Dual Link System-HRP (DAB+) kit (DAKO, Carpinteria, CA, USA). Following fixation in 95% ethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The average sample fragment length and purity was determined using the Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). After passing quality control measures ...
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bioRxiv - Biophysics 2021Quote: ... Six mutants of TDP-43 PLD were further generated by use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, United States) (16).
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bioRxiv - Cell Biology 2020Quote: ... while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat. 50671513, Agilent Technologies, Santa Clara, CA, USA) as well as RNA integrity (RIN) ...
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bioRxiv - Cell Biology 2021Quote: ... Coding regions and intron/exon boundaries were sequenced on the Novogen platform (agilent v6, HiSeqX,) after enrichment with Agilent kits (Agilent Technologies, Wokingham, UK). Exomes data were analysed using a bioinformatics pipeline developed in-house using two modules ...
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bioRxiv - Immunology 2021Quote: ... in order to modify its DAG discrimination capacity 17 by replacing Gly652 with Ala (G652A) using the Quick-Change XL site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the oligonucleotides described in Table 1 ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Immunology 2021Quote: ... cDNA amplification was carried out at 13 cycles of amplification for all samples and assayed for quality and concentration using a Bioanalyzer 2100 and High Sensitivity DNA Kit (Agilent, Santa Clara, CA).
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bioRxiv - Genetics 2020Quote: ... a natural NcoI restriction enzyme site located within intron 3 of the SPINK1 gene in the context of the previously constructed Mut expression vector was firstly eliminated by means of the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Les Ulis, France) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The average length of the indexed cDNA libraries was determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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bioRxiv - Genetics 2020Quote: ... Variants were introduced into the wild type constructions using the Quick-change II XL site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA). Presence of an abnormally spliced transcript associated with the decrease of the normal transcript was considered as “Impact on splicing” ...
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bioRxiv - Microbiology 2020Quote: ... libraries were checked for quality and size distribution using the 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, Santa Clara, CA, USA). Library quantitation was performed by Qubit using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Total amounts and the integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Transcriptomic analysis was performed by Novogene (Beijing ...
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bioRxiv - Cell Biology 2022Quote: ... were introduced into the plasmid pED-FLAG-LMAN1 and pcDNA-MCFD2-Myc separately using the QuickChange site-directed mutagenesis II XL kit from Agilent (Santa Clara, CA). FVIII mutant constructs Δ807–816 (deletion of amino acids 807-816 of FVIII ...
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bioRxiv - Cell Biology 2022Quote: ... or C166A/C180A in the GABAA receptor α1 subunit) were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). A FLAG tag was inserted between Leu31 and Gln32 of the α1 subunit and between Asn28 and Asp29 of the β2 subunit of GABAA receptors by using QuikChange II site- directed mutagenesis ...
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bioRxiv - Genetics 2022Quote: ... sheared genomic DNA was concentrated using a 0.6X v/v ratio of AMPure PB beads and libraries were sized using a Bioanalyzer 2100 instrument with the DNA 12000 Kit (Agilent Technologies, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality and quantity of the RNA was verified using an Agilent RNA 6000 Nano Kit on an Agilent Bioanalyzer (Agilent Technologies, California, USA) and a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Basal oxygen consumption rate and mitochondrial Oxidative Phosphorylation System (OXPHOS) parameters were measured according to manufacturer’s instructions of the Mito Stress test Kit (Seahorse Bioscience, Agilent Technologies, CA, USA). Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The average sample fragment length and purity was determined using Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). After passing quality control measures for loading the sequencing machine ...
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bioRxiv - Plant Biology 2022Quote: ... RNA integrity and quantitation were monitored with the RNA Nano 6000 Assay Kit (Cat #5067-1511) of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Following quality control ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-TDP-4FL was generated by introducing F147/149/229/231L mutations using site directed mutagenesis with QuickChange XL kit according to the manufacturer’s protocol (Agilent Technologies, Santa Clara, CA) into GFP-TDP-43 plasmid (gifted from CNDR).
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bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration and integrity was consecutively analyzed by capillary electrophoresis using a Fragment Analyser (Advanced Analytical Technologies, Inc., USA) and the Agilent RNA 6000 Pico Kit (Agilent, Cat# 5067-1513). RNA samples with RNA integrity numbers (RIN ...
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bioRxiv - Genomics 2022Quote: ... and its profile was assessed using a DNA High Sensitivity LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was paired-end sequenced on the Illumina HiSeq2500 (Illumina ...
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bioRxiv - Physiology 2022Quote: ... The dynamin-2 mCherry mutations A618T and S619L were constructed by site directed-mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Texas, USA). (DYN2 isoform aa ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration and quality (RIN > 8.0) was evaluated using an RNA 6000 Pico Kit and a 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, California). RNA sequencing was performed by the NYU Genome Technology Center ...
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bioRxiv - Plant Biology 2024Quote: ... and RNA integrity and concentration were measured using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 System (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2024Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The average length of the indexed cDNA libraries was determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and the RNA integrity and concentration were assessed using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Site-directed S586A mutagenesis was performed on the Kv2.1 channel using the Quick-Change XL Site-directed Mutagenesis kit (Stratagene, La Jolla, CA, USA). Kv2.1S586A mutation was confirmed by sequencing.