Labshake search
Citations for Agilent :
7551 - 7600 of 8613 citations for Testosterone ELISA Kit 1 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Point mutations were introduced into ATP8B2 and ATP8B4 using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and/or the SLiCE cloning method (Zhang et al ...
-
bioRxiv - Bioengineering 2023Quote: ... The RNA quality was evaluated using a 5200 Fragment Analyzer System and an Agilent HS RNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were constructed Using MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
-
bioRxiv - Cancer Biology 2023Quote: ... The total amount and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
-
Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The total RNA quantity and purity were analyzed by using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). RNA-seq libraries were constructed and then sequenced using an Illumina Novaseq™ 6000 (LC-Bio Technology ...
-
bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
-
bioRxiv - Systems Biology 2023Quote: ... quality check and quantification of total RNA was done using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 pico kit (Agilent Technologies, 5067-1513). Total RNA library preparation was done by introducing 500 ng total RNA into Illumina’s NEBNext Ultra II directional mRNA (UMI ...
-
bioRxiv - Physiology 2023Quote: ... Quality control of the total RNA submissions was completed using the Agilent TapeStation 4200 using the RNA ScreenTape Assay kit (Agilent Technologies 5067-5576). Total RNA (500ng ...
-
bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies, Santa Clara, CA, USA) to determine the length distribution of the fragments ...
-
bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
-
bioRxiv - Genomics 2022Quote: ... we assessed the quality of the RNA by quantifying the RNA integrity number (RIN) for each sample using the Agilent RNA 6000 Pico kit (Agilent, Santa Clara, CA) on the Agilent 2100 Bioanalyzer (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: ... The human GABAA receptor α1 subunit missense mutations (S76R, R214C, D219N, G251D, P260L, M263T, T289P, and A322D) were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). Enhanced cyan fluorescent protein (CFP ...
-
bioRxiv - Physiology 2023Quote: ... and the quality (e.g. fragment length) was assessed for a subset using a Bioanalyzer High Sensitivity DNA chip kit (Agilent Technologies, Santa Clara, CA). Single-end sequencing with 100-bp read length was conducted on a NovaSeq platform (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... quantitation and sample purity were assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and agarose gel electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... Beads-based double size selection was performed to ensure the fragment size of each sample was between 300-600 bp as measured by an Agilent DNA High Sensitivity NGS Fragment Analysis Kit (Agilent, DNF-474-0500). The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems ...
-
bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2023Quote: ... and leucine (Leu41) within the conserved destruction box were mutagenized to alanine (RxxL-> AxxA) using the Quickchange mutagenesis kit (Stratagene, La Jolla, CA). Worms were transformed with the pJK3 plasmid carrying gfp::ify-1dm using microparticle bombardment as previously described (Praitis et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... The integrity and concentration of the RNA were accurately measured using the Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). The detection indicators included RIN value ...
-
bioRxiv - Genomics 2023Quote: ... Libraries were eluted in 15µL H20 and concentrations measured with an Agilent 2100 Bioanalyzer using an Agilent HS DNA kit (Agilent Technologies, Cat# 5067-4627). Barcoded libraries of PV+ ...
-
bioRxiv - Plant Biology 2024Quote: ... and RNA integrity and concentration were measured using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 System (Agilent Technologies, CA, USA). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
-
bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
-
bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
-
bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
-
bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
-
bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
-
bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
-
bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
-
bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 µg of plasmid ASAP3 and ASAP3-R3 were added into the solution of GeneJammer (Agilent) transfection reagent in Opti-MEM (3 µL in 200 µL) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, Dako, Japan) and HRP goat anti-rat IgG antibody (Biolegend ...
-
bioRxiv - Microbiology 2019Quote: ... This was followed by incubation with the primary antibody (diluted 1:1,000 in dilution buffer; Dako) for 60 min at room temperature (RT) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were permeabilized using 0.2% Triton X-100 (Fisher) in 1:10 dilution Blocking Buffer (Dako) for 30 minutes at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... then stained with: rabbit polyclonal (pAb) glial fibrillary acidic protein (GFAP,1:1000, Dako, Z033401-2), goat pAb glial fibrillary acidic protein (GFAP,1:300 ...
-
bioRxiv - Molecular Biology 2020Quote: ... of which 1 μl was examines on a Bioanalyser high sensitivity DNA chip (Agilent 5067-4626). Ideal cycle number should bring final library to final concentration of 1-3 nM ...
-
bioRxiv - Molecular Biology 2021Quote: ... of which 1 μl was examined on a Bioanalyser high sensitivity DNA chip (Agilent 5067-4626). Ideal cycle number should bring final library to final concentration of 1-3 nM ...
-
bioRxiv - Molecular Biology 2020Quote: ... US Biological Life Sciences) and secondary antibody Rabbit Anti-Goat Ig/HRP (1:5,000, #P0160, Dako). Immunoblotting for α-Tubulin was performed using polyclonal Rabbit Anti-α-Tubulin antiserum as primary antibody (1:4,000 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies and dilutions used are as follows: guinea pig anti-insulin (DAKO, A0564, 1:1000), mouse anti-glucagon (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-PALB2 full-length and fragments were purified from 1 L of ArcticExpress cells (Agilent Technologies), grown at 37°C in LB broth medium containing 50 µg/mL ampicillin and 25 µg/mL gentamycin ...
-
bioRxiv - Neuroscience 2019Quote: ... The Western blot was stained with DAKO-Tau (1:10 000, Dako/Agilent, cat no A0024), while the second gel was stained with Colloidal Coomassie Staining solution (0.1% Coomassie Blue G250 ...
-
bioRxiv - Neuroscience 2019Quote: ... The Western blot was stained with DAKO-Tau (1:10 000, Dako/Agilent, cat no A0024), while the second gel was stained with Colloidal Coomassie Staining solution (0.1% Coomassie Blue G250 ...
-
bioRxiv - Neuroscience 2019Quote: ... GFAP immunohistochemistry for astrocytes (rabbit anti–mouse GFAP polyclonal antibody 1:13000 for 24 min; DAKO) and IBA-1 for microglia (rabbit polyclonal antibody 1:1000 for 24 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... Tissue sections were incubated with primary antibodies to anti-human Ki-67 (Clone MIB-1, DAKO) and cleaved caspase-3 (Cell Signaling ...
-
bioRxiv - Physiology 2020Quote: ... BrdU positive cells were detected with an anti-BrdU primary antibody diluted 1:100 (DAKO Cytomatation) and with an anti-mouse-Alexa488 conjugated diluted 1:100 (Thermofisher) ...
-
bioRxiv - Biochemistry 2020Quote: ... Secondary antibody was polyclonal goat α rabbit IgG HRP conjugate (Dako, lot 20066477, 1:1000 dilution). ECL (Amersham Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... This was followed by incubation with the primary antibody (diluted 1:6,000 in dilution buffer; Dako) overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... NUP210 primary antibody (Atlas) was applied at a dilution of 1:100 in antibody diluent (Dako) for 1 hour at room temperature ...