Labshake search
Citations for Agilent :
701 - 750 of 3073 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
-
bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
-
bioRxiv - Neuroscience 2021Quote: The exome sequences were enriched from 1.0 μg genomic DNA using an Agilent liquid capture system (Agilent Sure Select Human All Exon V5). First ...
-
bioRxiv - Cell Biology 2019Quote: ... KLF4 and C-MYC open reading frames under the control of the human elongation factor-1α (EF-1α) promoter in the presence of 1.2% GeneJammer (a polyamine-based transfection reagent; Stratagene, La Jolla, CA, USA). Cells were maintained in mTeSR™1 (STEMCELL Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Overexpjression of MTf was accomplished by stably transfecting SK-MEL-28 cells with human MTf cDNA or with the empty pCMV-Script® (Stratagene, CA, USA) vector alone14 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: Hybridization of samples was conducted on Agilent Whole Human Genome 4×44-k 60-mer oligonucleotide arrays (G4112F, Agilent Technologies, Santa Clara, CA), using 150ng of total RNA as template/sample ...
-
bioRxiv - Cancer Biology 2024Quote: ... WES libraries were prepared and captured using an Agilent SureSelect Human All Exon kit (Catalog No. 5191-5735, Agilent Technologies, Santa Clara, CA). The DNA library ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-AIF-1/Iba1 (1:1000, 019741 DAKO); and secondary antibodies ...
-
bioRxiv - Pathology 2020Quote: ... and Ki-67 (1:100, MIB-1, Dako) in an Autostainer Link48 automated staining platform (Dako ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Ki67 (Dako, MIB-1, 1:100), mouse anti-DLX2 (Santa Cruz ...
-
bioRxiv - Cancer Biology 2021Quote: ... before staining at a previously optimised dilution (BCL-xL 1:500; cleaved Caspase 3 1:500; MCL-1 1:200) and visualised with Liquid DAB (Agilent, UK). Ki67 (Agilent #M7240 ...
-
bioRxiv - Genomics 2020Quote: The sequencing libraries were exome captured using the SureSelectXT Human All Exon V6 (for esophagus libraries) (Agilent, Santa Clara, CA, Cat. No. 5190-8864) or V7 (for libraries from other tissues ...
-
bioRxiv - Immunology 2022Quote: ... Gene expression analysis of Phago-MDMs and Phago+MDMs were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 28004) with two technical replicates for each sample ...
-
bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
-
bioRxiv - Genomics 2021Quote: ... exome enrichment was applied to the cDNA purified from the previous step to enrich spliced cDNA by using probe kit SSELXT Human All Exon V8 (Agilent, catalog no. 5191-6879) and the compatible reagent kit SureSelectXT HSQ (Agilent ...
-
bioRxiv - Cancer Biology 2022Quote: ... Genome-wide expression profiling was performed using the low-input QuickAmp labeling kit and human SurePrint G3 8×60K pangenomic microarrays (Agilent Technologies, Santa Clara, USA) as previously described (Merdrignac et al ...
-
bioRxiv - Immunology 2024Quote: ... 2,5.105 PMN were incubated with different treatments and then stained for 30 min with a specific mouse anti-human CD11b antibody conjugated with PE (Dako, Santa Clara, CA, USA) at 4°C for 30 min ...
-
bioRxiv - Pathology 2024Quote: Consecutive 4-μm slices of human-aspirated thrombi were immunohistochemically stained using antibodies against glycophorin A (erythrocyte marker, mouse monoclonal antibody, clone JC159; Dako/Agilent, Santa Clara, CA, USA), fibrin (mouse monoclonal antibody ...
-
bioRxiv - Neuroscience 2024Quote: ... was performed on blood and hiPSC genomic DNA using the SurePrint G3 Human CGH Microarray Kit 4 x 180K in accordance with the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA, USA). Probe positions are referred to human genome assembly GRCh37/hg19.
-
bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, rabbit, Dako; 1:1000, chicken, Millipore), MBP (1:500 ...
-
bioRxiv - Cancer Biology 2019Quote: ... clone MIB-1 (diluted 1:100; DAKO, Carpinteria, CA) at 4°C overnight ...
-
bioRxiv - Pathology 2020Quote: ... anti-HER2 (1:400 to 1:600, DAKO, Denmark), anti-PR antibody (DAKO ...
-
bioRxiv - Neuroscience 2023Quote: ... Iba1 (1:1,000, Wako) and GFAP (1:1,000, Dako) overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
-
bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...
-
bioRxiv - Genetics 2021Quote: ... and cultured chondrocytes of children (six girls and six boys) using catalog one-color microarrays (SurePrint G3 Human Gene Expression microarray, 8 × 60 k format; Agilent Technologies, Santa Clara, CA, USA). The data were analyzed using GeneSpring software (version 14.9 ...
-
bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...
-
bioRxiv - Genetics 2024Quote: ... Whole exome sequencing was performed on genomic DNA extracted from the peripheral lymphocytes of the patients and their families using the SureSelect Human All Exon Kit V6 (Agilent Technologies, Santa Clara, CA, USA) with sequencing on the NovaSeq 6000 platform (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: ... the expression of 2,459 mature miRNAs was investigated by the microRNA microarray analysis using SurePrint G3 8×60K Human miRNA Microarray chips (AMADID 70156; Agilent Technologies, Santa Clara, CA, USA).
-
bioRxiv - Pathology 2024Quote: Consecutive 4-μm slices of human-aspirated thrombi were immunohistochemically stained using antibodies against glycophorin A (erythrocyte marker, mouse monoclonal antibody, clone JC159; Dako/Agilent, Santa Clara, CA, USA), fibrin (mouse monoclonal antibody ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN, 1:20,000, Millipore, MAB377B; CD68, 1:1,000, Serotec, MCA1957S; GFAP, 1:10,000, Dako Z0334) were diluted in 0.3% Triton X in PBS and incubated for 12 hours at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
-
bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Ki67 (1:50, clone MIB-1, Dako, M7240), mouse anti-INSR (1:50 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Anti-Ki-67 mouse (DAKO, clone MIB-1, 1:50); Anti-Ki-67 rabbit (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... GFAP (Biolegend, 829401, 1:1500 and Dako, Z0334, 1:1500), Nestin (Aveslabs ...
-
bioRxiv - Cancer Biology 2023Quote: ... thyroid transcription factor (TTF-1) (mouse, M3575, 1:100; Dako), and p40 (rabbit ...
-
bioRxiv - Immunology 2023Quote: ... stained for H&E and HSV-1 (1:1000, Dako), and analyzed by a veterinary pathologist (Dr ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45: 1:1000, 70257 Cell Signaling Technology; CD3: 1:2000 A0452 Dako/Agilent) diluted in TBST+5% goat serum in a humid chamber overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... The genomic DNA from the positive control was resubmitted for WES analysis using the SureSelect Human All Exon V6 kit from Agilent (performed by BGI Genomics Co, China) to compare both WES approaches ...
-
bioRxiv - Bioengineering 2022Quote: ... and Vimentin (1:5000 Dako, Ref M0725, Clone V9, 1:5000) were similarly outsourced to Veterinary Diagnostic Services ...
-
bioRxiv - Molecular Biology 2022Quote: ... and mouse monoclonal Ki-67 (1:200, MIB-1, DAKO, Denmark). The secondary antibodies were donkey anti-rabbit IgG conjugated to Alexa Fluor 488 ...
-
bioRxiv - Immunology 2021Quote: ... or Allphycocyanin-labeled streptavidin (1:200 dilution, Agilent, Cat #PJ27S-1). Cells were washed once with FACS buffer ...
-
bioRxiv - Immunology 2020Quote: ... MPO (Dako; Cat. No. A0398 at 1:1000 for 1 hour), pSTAT3 (Cell Signaling ...
-
bioRxiv - Immunology 2022Quote: ... MPO (Dako; Cat. No. A0398 at 1:1000 for 1 hour), Iba-1 (BioCare ...
-
bioRxiv - Immunology 2023Quote: ... 1 μM antimycin and 1 μM rotenone (Agilent, Cat# 103708-100) sequentially ...
-
Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... 37 µL of 1:500 SA-PE (Agilent Technologies, PJ31S-1) was added as a detection agent ...
-
bioRxiv - Cancer Biology 2019Quote: ... Immunofluorescence double staining was carried out in 1% horse serum using rabbit anti-GT198 (1:150) and mouse anti-Ki67 (1:200, DAKO). Secondary antibodies were anti-mouse or anti-rabbit Alexa Fluor-conjugated antibodies (Invitrogen ...