Labshake search
Citations for Agilent :
701 - 750 of 2871 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 488 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: Universal Human Reference RNA (Stratagene) was treated with RNase-Free DNase Set (Qiagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human universal total RNA (Agilent) was used as a positive control ...
-
bioRxiv - Immunology 2023Quote: ... For human CD45 (Dako, M0701), CD19 (Abcam ...
-
bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
-
bioRxiv - Biophysics 2022Quote: All of mutations in this study were made by using overlap-extension polymerase chain reaction (PCR) with Pfu polymerase (Stratagene) on the template of the mbr5 splice variant of mslo1 (Uniprot ID ...
-
bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
-
Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent). Sanger sequencing (Microsynth ...
-
bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: AMACR/p63 immunohistochemistry (IHC) double staining (Monoclonal Rabbit Anti-Human AMACR, clone 13H4, Dako, Code M361601-2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
-
bioRxiv - Developmental Biology 2019Quote: ... incubated for 1 hour at room temperature with biotinylated goat anti-mouse or goat anti-rabbit IgG antibodies (Dako, 1:500), washed 3 times and incubated with streptavidin Cy3 (Sigma ...
-
bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... Slides were then washed in TBST and incubated with Dako envision anti-rabbit or anti-mouse labelled polymer-HRP (Dako, USA) secondary antibody for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit (used at dilution of 1:2,000; Dako UK, Ltd.). Reactive proteins were visualized by chemiluminescence with ECL plus (Amersham plc).
-
bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated antibodies were used as secondary antibodies (Swine anti-rabbit 1:3000, Goat anti-mouse 1:5000, Dako, Glostrup, Denmark). Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories ...
-
bioRxiv - Physiology 2022Quote: ... the HRP-Anti-Rat IgG (MP-7444, Vector) for 45 min or the Goat Anti-Mouse Immunoglobulins/HRP (Dako-Agilent, P0447) at 1:100 for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies for 1 h at RT (Goat-anti-Rabbit-HRP, Goat-anti-Mouse-HRP, both Dako Denmark). Marker lanes were incubated with Streptavidin-biotinylated-HRP (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with anti-rabbit or anti-mouse horseradish peroxidase- labelled secondary antibody (Dako EnVision+ System- HRP Labelled Polymer) followed by a chemiluminescent reaction (Clarity Western ECL Substrate ...
-
bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
-
bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
-
bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed 3 times for 10 minutes each with TBST and probed with appropriate HRP secondary antibody (anti-Rabbit Immunoglobulin/HRP, Dako P044801 or anti-mouse Immunoglobulin/HRP, Dako P0260) at 1:1000 in TBST with 5% skim milk ...
-
bioRxiv - Pathology 2024Quote: Consecutive 4-μm slices of human-aspirated thrombi were immunohistochemically stained using antibodies against glycophorin A (erythrocyte marker, mouse monoclonal antibody, clone JC159; Dako/Agilent, Santa Clara, CA, USA), fibrin (mouse monoclonal antibody ...
-
bioRxiv - Microbiology 2020Quote: ... we used for western blot – a goat anti-mouse immunoglobulins HRP conjugated (P0447, Dako, Germany) or anti-rabbit (P0399 ...
-
bioRxiv - Neuroscience 2021Quote: ... UK) overnight and anti-mouse IgG alkaline phosphatase-linked secondary antibody (1:5,000; Dako, USA). Membranes were incubated with CDP-Star chemiluminescent substrate (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies coupled with horseradish peroxidase (HRP): goat anti-mouse (Dako P0447, WB: 1/1000), goat anti-rabbit (Dako P0448 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Goat anti-mouse polyclonal antibodies conjugated with horseradish peroxidase (cat. #P0447, Agilent, Santa Clara, USA) were used as secondary antibodies.
-
bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by a 1:150 dilution of rabbit anti-mouse immunoglobulins (Agilent/Dako, Glostrup, Denmark) for an hour ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by a 1:150 dilution of rabbit anti-mouse immunoglobulins (Agilent/Dako, Glostrup, Denmark) for an hour ...
-
bioRxiv - Microbiology 2021Quote: ... 10 000 RU (response units) of polyclonal anti-mouse antibody (No. Z 0420; Dako, Denmark) or polyclonal anti-human antibody (I2136 ...
-
bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
-
bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by 1-hour incubation with peroxidase-labeled goat anti-mouse antibodies (1:1000; DAKO) and a 7 min incubation with the True Blue™ (KPL ...
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated with horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary Abs (Dako) for 1h at RT and developed using Immobilon(tm ...
-
bioRxiv - Molecular Biology 2021Quote: ... Specimens were incubated with mouse-anti-SMA primary antibody (Actin Clone 1A4, Dako, Hamburg, Germany) and corresponding biotinylated goat-anti-mouse secondary antibody (Dako ...
-
bioRxiv - Cancer Biology 2021Quote: ... the following antibodies were used: anti-panCK (mouse, 1:1,000; DAKO, M3515, Clone AE1/AE3), anti-PHH3 (rabbit ...
-
bioRxiv - Cancer Biology 2021Quote: ... Pan-cytokeratin (panCK) was detected by incubating the tissue with anti-panCK antibody (mouse, Agilent, M351501-2 ...
-
bioRxiv - Physiology 2022Quote: ... Tubulointerstitial cell proliferation was detected by a monoclonal mouse anti-PCNA antibody (Dako, Glostrup, Denmark), while collagen I and fibronectin positivity were detected with polyclonal anti-collagen I (Rockland Immunochemicals ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by polyclonal goat anti-mouse/ rabbit immunoglobulins horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) at appropriate dilutions ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with HRP-conjugated secondary antibody (Rabbit Anti-Mouse Immunoglobulins/HRP #p0260, Dako) for 1 hour at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... and HRP-conjugated anti mouse IgG raised in goat (Dako-Agilent #P0447, dilution 1:500).
-
bioRxiv - Cell Biology 2020Quote: ... and HRP-conjugated anti mouse IgG raised in goat (Dako-Agilent #P0447, dilution 1:500).
-
bioRxiv - Cell Biology 2020Quote: ... The secondary antibody used was a polyclonal goat anti-Mouse immunoglobulins/HRP (P0447, Dako-Agilent). Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468 ...
-
bioRxiv - Cell Biology 2020Quote: ... The secondary antibody used was a polyclonal goat anti-Mouse immunoglobulins/HRP (P0447, Dako-Agilent). Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468 ...
-
bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were incubated at room temperature for 1 h with polyclonal goat anti-mouse (Dako) or anti-rabbit (Dako ...
-
bioRxiv - Microbiology 2021Quote: ... Following secondary antibodies were used: HRP conjugated goat anti-mouse antibody (#P0447, Dako from Agilent), HRP conjugated goat anti-rabbit antibody (#P0448 ...