Labshake search
Citations for Agilent :
701 - 750 of 1271 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... The column used was Eclipse XDB-C18 column (4.6 x 150 mm 5 μm, Agilent) and eluted with a linear gradient of 0-100% MeCN/H2O with constant 10 mM TEAA additive ...
-
bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
-
bioRxiv - Plant Biology 2020Quote: ... were loaded onto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
-
bioRxiv - Biochemistry 2021Quote: ... and ZORBAX SB-C18 Analytical column (4.6 x 250 mm, 5 μm particle size; Agilent), respectively ...
-
bioRxiv - Microbiology 2021Quote: ... Amino acids were separated using a VF-5ms inert 5% phenyl-methyl column (Agilent Technologies). The oven temperature was constant at 120 °C for 5 min ...
-
bioRxiv - Microbiology 2021Quote: ... The 5-AIQC-tagged samples (1 μL) were individually injected on an UPLC column (Agilent ZORBAX RRHD Eclipse XDB C18 column ...
-
bioRxiv - Microbiology 2020Quote: ... Sections were washed with TBS-Tween 0.05% for 5 min and rabbit/mouse link (Agilent) was added to slides and incubated for 15 min at room temperature in the dark ...
-
bioRxiv - Cancer Biology 2019Quote: ... sections were incubated for 5 min at room temperature with Protein Block Serum-Free (Dako). The tissue sections were then incubated with the anti-249T-P pAbs or TpMab-1 mAb (1 ...
-
bioRxiv - Microbiology 2019Quote: ... followed by 5 minutes of re-equilibration at 0% B (Agilent 1290 Infinity LC system). Post-column ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were imaged using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) to collect baseline GFP intensity ...
-
bioRxiv - Biochemistry 2020Quote: ... or an Eclipse XDB-C18 column (150 mm x 4.6 mm, 5 μm Agilent technologies) and an in-line fluorescence detector (λex 473 nm ...
-
bioRxiv - Systems Biology 2021Quote: ... were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... fitted with a guard column (2.1 mm × 5 mm, 1.8 μm Agilent ZORBAX SB-C18) was used ...
-
bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse IgG (1.3 μg ml−1, 5% milk in PBST; Dako, P026002-2) or goat anti-rabbit IgG (250 ng ml−1 ...
-
bioRxiv - Cell Biology 2021Quote: ... were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
-
bioRxiv - Molecular Biology 2023Quote: ... and cell numbers were determined with the Cytation 5 (Biotek Instruments, Agilent, Winooski, VT USA). OCR and ECAR data were expressed as pmol/minute/103 cells and mpH/minute/103 cells ...
-
bioRxiv - Neuroscience 2023Quote: Slide scanning and quantification were done using a Cytation 5 from Agilent (Cat No. BTCYT5PW) with GEN5PRIME software (Cat No ...
-
bioRxiv - Neuroscience 2023Quote: ... A Zorbax Eclipse XDB-C18 analytical column (5 µm, 4.6 x 150 mm) from Agilent Technologies was used ...
-
bioRxiv - Pathology 2023Quote: ... with a reserved-phase column (Li Chrospher 100-5 RP18 250 × 4.0 mm, Agilent Technologies), DAD (Agilent 1260 377 Infinity II DAD WR ...
-
bioRxiv - Pathology 2023Quote: ... Antigen retrieval was carried out using Proteinase K (5 μg/ml, 7 mins, Dako, UK). Sections were rinsed in running tap water for 5 min ...
-
bioRxiv - Bioengineering 2023Quote: ... capillary column (0.25 μm film thickness) HP-5MS (5% diphenyl 95% dimethylpolysiloxane from Agilent Technologies). Working conditions in the sample were as follows ...
-
bioRxiv - Cell Biology 2022Quote: ... at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent). Primary antibodies used herein are listed in Table 2 while secondary antibodies used are ...
-
bioRxiv - Cancer Biology 2023Quote: ... and washed 5 more times with PBS before mounting in Fluorescence mounting medium (#S3023, Dako). Z-stack images were acquired using the DeltaVision Elite widefield microscope (GE Healthcare Life Sciences) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and day 5 (96 hrs) by flow cytometry using a NovoCyte Quanteon flow cytometer (Agilent). The gating strategy is described in Suppl ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... with a HP-5 Ultra Inert column (Agilent, 117 30 m × 0.25 mm, 0.25 µm). 1μl of each sample was injected using Agilent ALS 7694 autosampler in split mode with a 5:1 ratio with helium as the carrier gas (250°C injector temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... and heated for 5 minutes (40°-70°C) in a PCR machine (Agilent SureCycler 8800). Next ...
-
bioRxiv - Cell Biology 2024Quote: 96-well plates containing single-cell clones were imaged on a Cytation 5 microscope (Agilent) 10 days after sorting ...
-
bioRxiv - Cancer Biology 2024Quote: ... Each cycle included a 5 min blocking step with serum free Protein Block (Agilent, #X0909), primary antibody incubation for 30 min ...
-
bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
-
bioRxiv - Genetics 2021Quote: ... and 5081) were used for whole-exome sequencing prepared using the SureSelect Human All Exon V7 probe set (Agilent, Design ID S31285117) per the manufacturers protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA qualities of the human RNA samples (RNA Integrity Number; RIN score) were assayed with high sensitivity RNA Screen Tape (Agilent 5057-5579) and shown in Table S4 ...
-
bioRxiv - Immunology 2021Quote: ... and bound serum IgG was detected by 70 µL/well of 1:3000 diluted rabbit anti-human IgG antibody linked to horseradish peroxidase (Agilent, P021402-2) incubated for 2h at RT ...
-
bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
-
bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
-
bioRxiv - Immunology 2021Quote: ... Paraffin sections (4µm thick) of FFPE thymus and lymph node tissues (NMR, mouse, and human control) were stained for cytokeratin (AE1/AE3, Dako GA05361-2) on a Dako Omnis autostainer with pressure cooker antigen retrieval (TrisEDTA ...
-
bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...
-
bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
-
bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA from isolated RNA as well as a Universal Human Reference RNA that was used as negative control or for normalization (HREF, Stratagene/Agilent # 740000) was performed using an oligo-dT primed SuperScript III Reverse Transcriptase (Invitrogen ...
-
Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: Mutagenesis of His 359 and Glu 363 to Alanine in human MYC (MycHEA mutant) was performed using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies # 200519), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: The hydrophilic N-terminal region of CLN6 was screened against a human fetal brain library using Stratagene’s Cytotrap yeast two-hybrid system (Y2H) as previously published (Stratagene, La Jolla, CA). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: ... Whole-exome sequencing libraries were prepared using SureSelectXT Human All Exon V5 + UTR kit (75 Mb; Agilent technologies, Santa Clara, CA, USA) as per manufacturer’s instructions and sequenced on HiSeq 2000 to generate paired end 2 × 100bp sequence reads (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
-
bioRxiv - Genetics 2020Quote: Exome and clinical exome sequencing was performed on 3μg of genomic DNA using the Agilent SureSelectXT Human All Exon (V6) and Focussed Exome respectively (Agilent Technologies, CA, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... the 3 liver cancer specimens also underwent bulk-level WES using Agilent SureSelect Human All Exon v7 K it (Agilent, 5191-4005) and illumina NovaSeq 2 × 150 bp sequencing mode ...
-
bioRxiv - Molecular Biology 2023Quote: ... Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949 ...
-
bioRxiv - Genetics 2023Quote: Exome enrichment was applied to the cDNA purified from the previous step by using probe kit SSELXT Human All Exon V8 (Agilent, 5191-6879) for human samples ...
-
bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...