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Citations for Agilent :
701 - 750 of 3323 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Membranes were washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako, P0448/P0447) at room temperature for 1 h (1:2000 in 0.3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The cleared lysates were loaded on wells of 96-well single-use filter micro-plates with 3 µm glass fibers and 25 µm polyethylene membranes (Agilent, 204495-100) containing the HB95 cross-linked beads ...
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bioRxiv - Genomics 2023Quote: ... cDNA was diluted to approximately 3 ng/μL and run on the 2100 Agilent Bioanalyzer using the High Sensitivity DNA Kit (Agilent 5067-4626). The measured fluorescence units in Fig 1b have been corrected for the dilution factor.
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bioRxiv - Cancer Biology 2023Quote: ... treated with 3% H2O2 to block endogenous peroxidase then incubated in a steamer for 30 minutes in Target Retrieval Solution (Dako, #S1699, #S2367) for antigen retrieval ...
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bioRxiv - Cell Biology 2023Quote: ... TMEM24(ΔBS + 5S → E)-eGFP was generated using site-directed mutagenesis to remove a portion of the C-terminus of TMEM24(5S → E)-eGFP including the first 3 β-strands of the β-sheet band 4.1 interacting domain (Quik-Change II XL; Agilent Technologies).TMEM24(5S → E)-eGFP which has been previously described (Sun et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako, P0448/P0447) at room temperature for 1 h (1:2000 in 0.3% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... samples were centrifuged at 20.800 xg for 2 min and 100 µl sample was injected on a SEC-HPLC column (Bio SEC-3 300 Å, Agilent, USA) using an Agilent 1260 Infinity II system (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were permeabilized with 0.1% Triton x-100 (Fluka, 93426) and blocked with the blocking solution containing 3% Goat serum (Dako, Cat. no. X0907), 0,1% sodium azide (the healthcare business of Merck KGaA ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse primer (5’-AGGTCATGTACTGGGCATAAT-3’) binding to the GFP sequence with 5 µL of Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, USA) and 0.15 µL of 2 µM reference dye.
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the supernatant was removed before exposing the plate to a temperature gradient for 3 minutes in a PCR machine (Agilent SureCycler 8800), followed by 3 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, 103774-100). Cells were washed in PBS once and incubated in a hypoxic chamber following the manufacturer’s procedure ...
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bioRxiv - Molecular Biology 2024Quote: Serial sections of human and mouse FFPE BM biopsies were prepared at 3-5 μm thickness on coated microscope slides (Dako FLEX, Agilent) and processed for immunohistochemistry/immunofluorescent-based (IHC-IF) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed in TBST (3 × 15 min at RT) and then incubated with corresponding peroxidase-conjugated secondary antibodies (Dako, P0447, P0448) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN, 1:20,000, Millipore, MAB377B; CD68, 1:1,000, Serotec, MCA1957S; GFAP, 1:10,000, Dako Z0334) were diluted in 0.3% Triton X in PBS and incubated for 12 hours at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated with the indicated primary antibodies (Reagents) overnight at 4 °C followed by Envision/diaminobenzidine detection (Dako, Glostrup, Denmark) and hematoxylin counterstaining/mounting (Entellan ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Genomics 2021Quote: ... 4 μl of the purified product were amplified in multiple 100 μl reactions using Herculase II Fusion DNA Polymerase (Agilent) following the manufacturer’s specifications with 0.3 μM of the IS5/IS6 primers ...
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... iPSC-derived kidney organoids were cut at a thickness of 4 μm using a cryo-microtome and mounted on FLEX IHC Microscope Slides (DAKO, Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Each RNA sample was processed with fluorescence-labelling to prepare complimentary RNA (cRNA) targets that could be detected by an SBC human ceRNA array V1.0 (4□×□180K, Agilent Technologies). The microarray kit contained 88,371 circRNA probes and 18,853 mRNA probes ...
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bioRxiv - Microbiology 2021Quote: Headspace CO concentrations were monitored every 2-4 days using an Agilent 6890N gas chromatograph (GC; Agilent Technologies, California, US) equipped with a nickel catalyst methaniser (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... esters and 4-vinyl guaiacol concentrations were analysed using headspace solid phase micro-extraction coupled with gas chromatography (Agilent 7890A)- mass spectrometry (Agilent 5975C ...
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bioRxiv - Microbiology 2021Quote: ... All other synthetic or expressed peptide substrates were purified and analyzed at small scale utilizing analytical-scale reverse-phase HPLC on an Agilent 1100 series HPLC system (Santa Clara, CA) employing an Eclipse Plus C18 column (5 μm, 4 × 150 mm) (Agilent) at a flow rate of 1 mL/min ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
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bioRxiv - Immunology 2020Quote: Extracts and 4-OHE1 standard were analyzed using a liquid chromatography system (LC; 1200 series, Agilent Technologies, Santa Clara, CA) that was equipped with a reversed-phase analytical column (length ...
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bioRxiv - Cancer Biology 2022Quote: Cells were imaged using a 60x Plan Apo 1.4 NA objective on a Nikon Ti2-E microscope with a Yokogawa X1 spinning disk confocal system, MLC400B 4-line (405nm, 488nm, 561nm, and 647nm) dual-fiber laser combiner (Agilent), Prime 95B back-thinned sCMOS camera (Teledyne Photometrics) ...
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bioRxiv - Microbiology 2022Quote: ... One hundred μL of the extract was mixed with 10μL of 4-chlorobenzotrichloride as injection standard and analyzed by GC (HP 7890 Series GC, Agilent, USA) with a 20 m 0.18mm (18μm film thickness ...
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bioRxiv - Biochemistry 2024Quote: ... and 3D z-stacks were taken of each replicate well monitored every 4 hours for 48 hours using a Cytation C10 imager (Agilent) with confocal 546 nm excitation laser ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Cancer Biology 2023Quote: ... The adapter-modified DNA fragments were enriched by 4 cycles of PCR using SureSelect forward and SureSelect ILM Pre-Capture Indexing reverse (Agilent) primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with Alamar Blue for 3-4 hours and then imaged with a Synergy Neo plate reader using excitation: 550 nm and emission: 590 nm (Agilent). The average plate background (media only with 0.1% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... This system makes it possible to culture many bacterial strains in parallel in a replicated manner using many 96-well plates which are handled with a stacker (BioStack 4, Agilent Technologies ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Microbiology 2023Quote: ... Growth curves of OD578 were monitored every hour after 1 min of linear shaking under anaerobic conditions using an Epoch2 microplate reader coupled with a Biostack 4 microplate stacker (both Agilent) housed in a custom-made incubator (EMBL workshop24) ...
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bioRxiv - Biochemistry 2024Quote: ... mutations were inserted into the WT isolated (residues 193 – 261) RRM2 domain in a modified pGEX-6p1 vector (4) using the QuickChange method (Stratagene) and verified through Sanger Sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and quantity of the enriched libraries were evaluated using Qubit® 4 Fluorometer and 4200 TapeStation System (Agilent). Sequencing was performed on an Illumina NovaSeq6000 machine with single-end 100 bp reads for NPM1 and H3K9me2 according to standard protocols.
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... and diluted to 3.5% nitric acid by adding 3800 μL milliQ water after which all test tubes containing samples were placed into an automatic sampler (SPS 4 Autosampler, Agilent) integrated with the MP-AES (model 4210 ...
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bioRxiv - Physiology 2024Quote: ... RNA concentration and integrity were evaluated by a fluorimetric assay (Qubit 4, ThermoScientific, USA) and capillary electrophoresis (TapeStation, Agilent, Germany), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... Temperature-dependent phase transition was detected by measuring the optical scattering at 800 nm while increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface ...
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Nine images were taken every 4 hours in each well and the number of cells by images were determined using BioTek Gen5 Software (Agilent).
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bioRxiv - Immunology 2024Quote: 0.4 ug of biotinylated SL9 (SLYNTAVTL) or IV9 (ILKEPVHGV) peptides (NIH Tetramer Core Facility) were conjugated to PE-streptavidin (Agilent), APC-streptavidin (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated for each aaRS activity ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated as the GFP synthesis rate ...
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were automatically taken every 4 hours over 72 hours in a BioTek BioSpa Automated Incubator (Agilent, Santa Clara, CA). Cell proliferation was quantified using Aligent’s Gen5 image analysis tools.
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bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Ki67 (1:50, clone MIB-1, Dako, M7240), mouse anti-INSR (1:50 ...