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Citations for Agilent :
7301 - 7350 of 7968 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The L834R mutation was introduced in EGFR using the QuikChange II Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Agilent Technologies, #200523). The plasmid used for the neural network training encoded for the extracellular and transmembrane domain (ECTM ...
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bioRxiv - Microbiology 2022Quote: ... C194 in NlpC/P60Ela and C153 in in NlpC/P60Sne were changed to alanine using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). The respective sense and antisense primers listed in Supplementary Table 1 were used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using the broad range RNA assay and rudimentary cDNA quality and size information was obtained from an Agilent 2100 Bioanalyzer with a Broad Range DNA Kit (Agilent, USA). For Qubit conversions from ug to picomoles cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA isolated from jejunum was subjected to quality control analysis using an Agilent 2100 Bioanalyzer with RNA 6000 Nano Kits (Agilent, USA). After poly A enrichment the samples were fragmented and reverse-transcribed to generate complementary DNA for sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Required quality control steps and quantification measurements within this protocol were performed using the Agilent High Sensitivity DNA Kit (5067-4626, Agilent Technologies) and the KAPA Library Quantification Kit (2700098952 ...
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bioRxiv - Cell Biology 2023Quote: ... Probes (available in Table S7) were labeled with 32P-dCTP using the Prime-It RT random labeling kit (Agilent, catalog #300329) and hybridized to the membrane ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids harboring GlnR amino acid substitutions (pET28a-glnR derivatives) were constructed using site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit, Agilent, Inc.).DNA fragments consisting of different canonical GlnR-dependent promoters (narG whose downstream genes encoding M ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates from at least 3 independent biological samples using the SYBR green Brilliant II fast kit (Agilent Technologies) on an Mx3005p apparatus (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The T94D mutation was introduced in the plasmids by PCR using the QuickChange Multi-Site Directed Mutagenesis kit (#200514 -Agilent Technologies). The sequence of the primer used to introduce the mutation T94D in the Nme1 sequence of plasmids is reported in key resources table ...
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bioRxiv - Microbiology 2022Quote: ... was performed to remove adapter dimers and 1 μl of the cleaned pool was visualised using the Agilent High Sensitivity DNA Kit for Bioanalyzer 2100 (Agilent Technologies) to confirm adapter-dimer removal.
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bioRxiv - Neuroscience 2023Quote: ... concentration and molarity of the libraries were then measured with the Fragment Analyzer HS NGS Fragment Kits (Agilent, cat#DNF-474). All libraries were pooled equimolar and sequenced on NovaSeq6000 and NovaSeq control software NCS v1.7 and the Illumina data analysis pipeline RTA3.4.4 and Bcl2fastq v2.20 for the following image analysis ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and the concentrations and RNA Integrity Numbers (RIN) were determined with an RNA 6000 nano kit on a 2100 Bioanalyzer (Agilent Technologies). Libraries for TCR sequencing were prepared as described (55) ...
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bioRxiv - Neuroscience 2023Quote: ... The quality of each library was assessed by capillary electrophoresis on FragmentAnalyzer using the NGS High Sensitivity kit (Agilent, DNF-474). Sample libraries were pooled and sequenced on Illumina NovaSeq 2000 targeting 50,000 read pairs per tissue-covered spot.
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was examined using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA yield was determined using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Sample DNA concentrations were measured using the Qubit dsDNA HS kit and the DNA quality was evaluated using TapeStation with the Genomic ScreenTape (Agilent Technologies). Sequencing libraries were prepared using the NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and ACT1 were prepared by PCR from CLA1-2 genomic DNA using primers N1 to N10 (Supplementary Table S2d) and radioactively labeled by P32 with Random Primer Labeling Kit (Agilent, 300385). Blots were scanned using Image Quant 5.2 software (Molecular Dynamics).
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bioRxiv - Microbiology 2022Quote: 375W substitutions in all the primary isolates were introduced by site-directed mutagenesis in plasmids carrying Envs using a QuikChange site-directed mutagenesis kit (Stratagene Inc.) and mutagenic primers to introduce each mutation (56) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fragment size distributions of tagmented and dual indexed libraries were analyzed by capillary gel electrophoresis using a Bioanalyzer High Sensitivity DNA kit (50067-4626, Agilent, USA). Up to 384 high quality libraries with size distribution between 250-1000bp were pooled using equal molar quantities ...
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bioRxiv - Cell Biology 2023Quote: ... the integrity of DNAse I-treated RNA was determined using the Agilent RNA 6000 Pico Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RIN values > 4 was used for RT.
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bioRxiv - Cell Biology 2023Quote: ... TP53-missense mutations and protospacer adjacent motif (PAM) sequence mutation were introduced using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). A puromycin or neomycin resistance cassette was cloned into the BamHI site which was placed between left- and right-arm of the amplified and cloned DNA fragments ...
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bioRxiv - Genomics 2023Quote: ... The concentration and quality of the sample was evaluated with Qubit dsDNA HS kit and Tapestation D5000 Screen Tape (Agilent Technologies). The expected recovery was above 50 ng.
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Bioengineering 2023Quote: ... Specific site directed mutations on the S309 and CMAB0 antibody sequence was done using the Quick-change site directed mutagenesis kit II (Agilent technologies).
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The putative ESRRA binding site 1 was mutated using PCR-based site-directed mutagenesis and QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518) to obtain mutated constructs pGL3-Leptin mut 1-luc.
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bioRxiv - Microbiology 2023Quote: ... samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Systems Biology 2023Quote: ... The quality and quantity of all libraries were checked using Agilent’s Bioanalyzer 2100 and DNA 7500 kit (Agilent Technologies, 5067-1506).
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bioRxiv - Molecular Biology 2023Quote: ... The quality of RNA was evaluated using the Agilent 2100 Bioanalyzer with Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511). Libraries were prepared using the Illumina TruSeq Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina according to the manufacturer’s protocol and sequenced on an Illumina HiSeq 4000 (2× 75 bp).
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bioRxiv - Genomics 2023Quote: ... and their size range was checked by capillary electrophoresis using with the DNA high sensitivity kit and a Bioanalyzer 2100 (Agilent Technologies). Libraries were diluted to 1 nM and pooled equimolarly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was measured using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA yield was determined using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... keeping in parity the Y320 residue of Gαi3 and Gαs the Y320E and Y320F mutants were generated using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The mutagenesis of the HDE-like motifs and PAM sequence within donor plasmid for HDR was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). pcDNA3.1(+)-ARRDC4-1 and pcDNA3.1(+)-ADCYAP1-2 expression vectors were constructed by cloning lnc-ARRDC4-1 and lnc-ADCYAP1-2 sequences to pcDNA3.1(+ ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Genomics 2023Quote: ... cDNA concentration was measured using Qubit dsDNA HS kit and the size distribution analyzed using Tapestation D5000 Screen Tape (Agilent Technologies).
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bioRxiv - Synthetic Biology 2023Quote: ... The average length of the DNA libraries was evaluated using Agilent Bioanalyzer 2100 and a High Sensitivity Kit (Agilent Technologies, USA) and the concentration of the DNA libraries was determined with a Qubit device (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies, Cat. No. / ID: 5067-4626). Libraries were sequenced on an Illumina NextSeq2000 in paired end mode with the read length of 50 nt and sequencing depth of 25 million reads per library ...
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bioRxiv - Cell Biology 2023Quote: ... Supplementary Table S3c) or for Ptgs1 generated by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). (Agilent ...
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bioRxiv - Genomics 2023Quote: We purified the DNA using AMPure beads (Beckmann Coulter, USA) and assessed quality using a Large Fragment Analysis Kit (Agilent, USA). The library was prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Integrity and concentrations of the RNA samples were determined using an Agilent 2100 bioanalyzer with RNA 6000 Nano Kit (Agilent, USA). Libraries for 6 samples were prepared using the Ion Total RNA-Seq Kit v2 according to the manufacturer’s protocol using 12-22 polyA-enriched RNA as input material ...
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bioRxiv - Cell Biology 2023Quote: The quantity and quality of RNA samples was assessed using the standard sensitivity RNA fragment analysis kit on Fragment Analyzer (Agilent Technologies). All samples had an RNA integrity number >8 and were deemed of sufficient quantity and quality for RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and the quality assessed with an Agilent 2100 Bioanalyzer with an RNA 144000 Nano Kit (Agilent technologies, Palo Alto, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... The remaining topology mutants of UBIAD1 were generated using the QuikChange® site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and pCMV-Myc-UBIAD1 as a template.
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bioRxiv - Cell Biology 2022Quote: ... were assessed using the XF96 Extracellular Analyzer as described in manufacturer’s instructions for the XF cell mito stress and the XF cell mito fuel flex test kits (Agilent Technologies, CA).Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... The total cellular ATP production rate is the sum of glycolytic and mitochondrial ATP (ATP Rate assay Kit; 103592-100, Agilent technologies). For ECAR analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were quality controlled using a 2100 Bioanalyzer system with Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc., 5067-4626) and quantified with Qubit™ 1X dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality of sequencing libraries was validated with a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626). The pooled sequencing libraries were quantified and sequenced on either the MiSeq (v2 or v2 nano ...
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bioRxiv - Biophysics 2022Quote: ... Amber codon mutagenesis in the selected positions were performed using the QuickChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, California, USA) following the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: PTEN monoubiquitination mutants PTEN-K13R and PTEN-K289R were generated from GFP-PTEN by site directed mutagenesis using QuickChange XL Site Directed Mutagenesis Kit (Agilent technologies). Table S1 includes the primer sequences.