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Citations for Agilent :
651 - 700 of 1418 citations for 3 Hydroxy 20 oxo 5 pregnen 17alpha yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and 5 µg/mL tRNA at room temperature for 25 min and UV cross-linked on ice at 254nm for 20 min in a Stratalinker (Stratagene), followed by digestion with RNase A/T1 mix (Ambion ...
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bioRxiv - Genomics 2020Quote: ... the amplified libraries were purified using a Qiagen MinElute PCR Purification Kit and eluted in 20 μl Elution Buffer before quantitation on an Agilent 4200 TapeStation (Agilent Technologies LDA UK Limited ...
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bioRxiv - Microbiology 2021Quote: ... One hundred microliters of this solution and 5□mL of deionized water or beer were placed in a 20 mL glass vial and sealed with a crimp cap (Agilent). The sample/standard vial was then placed in the headspace sampler with following conditions ...
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bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... Cat N° R37605) for 20 min and coverslips were mounted with Fluorescence Mounting Medium (Dako, Glostrup, Denmark, Cat N° S3023), while wells in the device were sealed with one drop of mounting medium per well before acquiring images ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were de-coverslipped with 1 min of agitation in TBST and subjected to heat-mediated antigen retrieval in 1x pH 6.0 citrate buffer (Biogenex Laboratories, HK0809K) for 20 min at 95°C followed by blocking of endogenous peroxidase activity (Dako, S2003 ...
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bioRxiv - Cancer Biology 2020Quote: ... trifluoroacetamide containing 1% trimethylchlorosilane (Pierce, 20 μl, 1 hour, 37 °C) and analysed by GC-MS using a VF5 capillary column (Agilent, 30 m ...
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bioRxiv - Microbiology 2021Quote: ... The quality of the DNAs was controlled determining the DNA Integrity Number (DIN) analyzing 20 ng of DNA on a TapeStation 4200 (Agilent). One µg genomic DNA was used to prepare a library for whole genome sequencing on an automated platform ...
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bioRxiv - Biochemistry 2022Quote: ... was taken up in 20 mM ammonium formate (pH 10) and prefractionated first into 29 fractions on a 1200 Infinity HPLC (Agilent) using high-pH reversed-phase chromatography (running buffer A ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with TBST (Tris Borate Saline Tween-20) and then blocked with Protein Block Serum-Free (Ref: No X0909, Dako) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then washed twice with wash buffer before a 20-minute incubation with Envision FLEX HRP Polymer (GV823, Agilent DAKO) for HRP binding ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
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bioRxiv - Bioengineering 2020Quote: ... for 20 minutes at room temperature before mounting with Dako Fluorescent mounting medium (catalog no. s3020; DAKO, Carpinteria, CA, USA). For histological assessment ...
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bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
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bioRxiv - Plant Biology 2020Quote: ... Extracted ion chromatogram (EIC) values were determined by extracting chromatograms with a 20 ppm error and integrating peak areas using MassHunter software (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... HPLC separation was performed by Shimadzu LC-20 AD Analytical System using UV (Agilent 1100 VW set at 253 nm) detection ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen unmasking was carried out in a DAKO PT-Link for 20 minutes at 97°C using EnVision FLEX buffer of appropriate pH for each antibody (Agilent). Then ...
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bioRxiv - Microbiology 2020Quote: ... five grams were sampled and placed in a vial (HS, crimb, FB, 20 ml, clr, cert, 100 PK, Agilent Technologies) hermetically capped by a pressure plug with a plastic membrane ...
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bioRxiv - Biophysics 2021Quote: ... sections were fixed for 10 min in cold acetone (−20 °C) and rinsed in TBS (Agilent, Santa Clara, CA, USA). For staining of MΦs ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Microbiology 2023Quote: ... samples were boiled in a pressure cooker for 20 min in citrate buffer pH 6.1 (Dako Target Retrieval Solution, S2369) and transferred to water ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes at room temperature with an anti-rabbit antibody coupled with horseradish peroxidase (HRP) (Flex, Dako). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μl of re-dissolved solution was then loaded into an injection vial (cat. 9301-0978, Agilent; equipped with a snap cap (cat ...
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bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Plant Biology 2024Quote: ... 500 mg (1 mL) of the powder was transferred immediately to a 20 mL head-space vial (Agilent, CA, USA), containing NaCl saturated solution ...
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bioRxiv - Physiology 2023Quote: ... Some 20 μl of muscle homogenates was transferred to a well of a Seahorse XF24 Islet Capture Microplate (Agilent Technologies). After placing an islet capture screen by a Seahorse Capture Screen Insert Tool (Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... Some 20 μl of muscle homogenates was transferred to a well of a Seahorse XF24 Islet Capture Microplate (Agilent Technologies). After placing an islet capture screen by a Seahorse Capture Screen Insert Tool (Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... A total of 20 μl of re-dissolved solution was then loaded into an injection vial (cat. 9301-0978, Agilent; equipped with a snap cap (cat ...
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bioRxiv - Molecular Biology 2024Quote: ... The elution was in 10-20 µL of RNase-free water and the RNA profiling and quantification was assessed by Agilent 2100 Bioanalyzer and RNA 6000 Pico Kit (5067-1513) ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then heated to 97°C for 20 minutes in a high pH target retrieval solution (TRS) (K8004, Agilent) for heat-induced antigen retrieval (HIER) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed between all experimental step with PBS plus 0.1% Tween 20 (405 TS ELISA Plate Washer, Agilent Technologies). Blocking (1% Omniblok ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Microbiology 2024Quote: ... Plates were parafilmed to reduce evaporation and cells were grown for 20 hours with shaking (807 cpm) at 37°C in a BioTek Synergy H1 microplate reader (Agilent). Optical density values are adjusted by the background value at T0.
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bioRxiv - Neuroscience 2024Quote: ... 475 nm laser output was pulsed at 15 Hz with a 20 ms pulse width using an external arbitrary waveform generator (Agilent) attached to the signal port of the laser ...
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bioRxiv - Biochemistry 2024Quote: ... sections were deparaffinized, dehydrated and subjected to antigen retrieval (20 min incubation at 98 °C in citrate buffer, pH 6 (Agilent Dako) for CD8 ...
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bioRxiv - Physiology 2024Quote: ... RNA was reconstituted in nuclease-free water (20-50 µL) and RNA quantity and quality was assessed using a bio-analyzer (2100 Bioanalyzer, Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... The slides were then incubated at 105 °C for 20 minutes in 1X DAKO high pH buffer (Agilent Technologies, Dako Target Retrieval Solution pH 9 10X ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were dewaxed and antigen retrieval process using citrate buffer pH6 for 20 min at 97°C using a PT Link (Dako – Agilent) was performed ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemical reaction was developed using 3, 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cell Biology 2020Quote: ... Frozen samples were spread to a thin layer on petri dishes pre-cooled on dry ice and UV-crosslinked 3 times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). All samples were stored at −80°C until tissue lysis.
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Developmental Biology 2020Quote: ... the included linker sequence between 3’ UTR and the start of sfGFP was removed using site-specific mutagenesis (QuikChange, Agilent). The gRNA expression plasmid and donor plasmid were injected by BestGene into embryos of Vas-Cas9 flies (#56552 ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...