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Citations for Agilent :
6701 - 6750 of 8383 citations for Human Uromodulin Like 1 UMODL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: RNA from sorted cells were analyzed on the Agilent 2100 Bioanalyser system using the Eukaryote Total RNA Pico kit (Agilent Technologies) and the mean RIN number was 9.5 ...
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bioRxiv - Neuroscience 2024Quote: ... and assessed for RNA integrity using an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). The sequencing libraries were then multiplexed and loaded onto the Illumina NovaSeq 6000 instrument’s flowcell ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 libraries were generated from murine tissue samples and libraries were quantified using the Agilent DNA 1000 kit (Agilent Technologies, USA).
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bioRxiv - Cancer Biology 2024Quote: ... and the library quality check has been performed using a High Sensitivity NGS Fragment Analysis Kit on a Fragment Analyzer System (Agilent Technologies). Indexed libraries were normalized and pooled to be sequenced on an Illumina Novaseq 6000 sequencer according to manufacturer’s instructions with targeted conditions of 2 x 75bp and 25M reads/sample.
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bioRxiv - Cell Biology 2024Quote: ... The quality of the RNA samples was assessed using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Kit, 5067-1511, Agilent technologies) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA integrity was initially assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and only RNA samples with an RIN value above 4 were selected for further processing ...
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bioRxiv - Immunology 2023Quote: ... The predicted CLIPA8 cleavage site 63IMLR66 was replaced by IEGR [26] using the CLIPA8mutag primer and the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to create plasmid pOET3-CLIPA8Xa-V5-His (Table S3) ...
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of each library was analyzed on the Bioanalyzer with a High Sensitivity DNA analysis Kit (Agilent technologies) and Collibri Library Quantification Kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of each library was analyzed on the Bioanalyzer with a High Sensitivity DNA analysis Kit (Agilent technologies) and Collibri Library Quantification Kit (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... we first inserted the SalI site at just downstream of the termination codon of the dsRed cDNA of pIRES2-dsRed-IRF418 using a QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies), and isolated the BglII-SalI fragment containing the mIRF4-IRES-dsRed DNA region from the mutated plasmid ...
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bioRxiv - Genomics 2024Quote: ... quality control was performed by run on an Agilent 2100 Bioanalyzer System using the High Sensitivity DNA Kit (Agilent, 5067-4626). Libraries were then created from each fraction using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Library pools concentrations were measured using Qubit dsDNA HS Assay Kit and the quality of the libraries was assessed by High Sensitivity D5000 ScreenTape (Agilent Technologies), followed by sequencing using Illumina NextSeq500.
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bioRxiv - Genomics 2024Quote: ... DNA quality and size were assessed using an Agilent 4200 TapeStation with a gDNA ScreenTape Kit (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2024Quote: ... Bevacizumab-sensitive/resistant U87 cells were cultured identically to wild-type U87 cells.79 Cells were screened for mycoplasma every 3 – 4 months with the MycoSensor qPCR Assay Kit (Agilent Technologies).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... RNA integrity was assessed using an RNA Nano 6000 Assay Kit on an Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Bioengineering 2024Quote: ... and extracellular acidification rate (ECAR) were measured following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent, Madison, WI). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Mutations in the DFCP1 (K193A and R266Q) and ATGL (D166G) were introduced using the QuikChange mutagenesis kit (Agilent, Santa Clara, CA).
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bioRxiv - Cell Biology 2024Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Biophysics 2023Quote: ... or K280C (T) as well as the C242A mutation were introduced into the pETM30 vector using the QuikChange® mutagenesis kit (Stratagene) (17) ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment was gel purified and used as template in error-prone PCR (EP-PCR) using the GeneMorph II kit and Mutazyme II polymerase (Agilent Technologies) and the primers (5RT 3RT) ...
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bioRxiv - Biophysics 2023Quote: ... site-directed mutagenesis was used using the vector pET21a-αSynuclein with the kit QuikChange XL Site-Directed Mutagenesis (Agilent technologies, USA) and the inserted mutations were checked by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Quality control checks and quantification were performed using an Agilent 2100 Electrophoresis Bioanalyzer instrument with the Agilent RNA 6000 Pico kit (Agilent Technologies), and a Qubit 2.0 fluorometer with the Qubit RNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... concentration and molarity of the libraries were then measured with the Fragment Analyzer HS NGS Fragment Kits (Agilent, cat#DNF-474). All libraries were pooled equimolar and sequenced on NovaSeq6000 and NovaSeq control software NCS v1.7 and the Illumina data analysis pipeline RTA3.4.4 and Bcl2fastq v2.20 for the following image analysis ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and the concentrations and RNA Integrity Numbers (RIN) were determined with an RNA 6000 nano kit on a 2100 Bioanalyzer (Agilent Technologies). Libraries for TCR sequencing were prepared as described (55) ...
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bioRxiv - Neuroscience 2023Quote: ... The quality of each library was assessed by capillary electrophoresis on FragmentAnalyzer using the NGS High Sensitivity kit (Agilent, DNF-474). Sample libraries were pooled and sequenced on Illumina NovaSeq 2000 targeting 50,000 read pairs per tissue-covered spot.
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was examined using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA yield was determined using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Sample DNA concentrations were measured using the Qubit dsDNA HS kit and the DNA quality was evaluated using TapeStation with the Genomic ScreenTape (Agilent Technologies). Sequencing libraries were prepared using the NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and ACT1 were prepared by PCR from CLA1-2 genomic DNA using primers N1 to N10 (Supplementary Table S2d) and radioactively labeled by P32 with Random Primer Labeling Kit (Agilent, 300385). Blots were scanned using Image Quant 5.2 software (Molecular Dynamics).
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bioRxiv - Microbiology 2022Quote: 375W substitutions in all the primary isolates were introduced by site-directed mutagenesis in plasmids carrying Envs using a QuikChange site-directed mutagenesis kit (Stratagene Inc.) and mutagenic primers to introduce each mutation (56) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutations G to C at positions 445 and 457 were introduced into DCP2 in plasmid pQZ145 (Zeidan et al. 2018) using primers AKV005/AKV006 and the Quick-change Site-Directed mutagenesis kit (Agilent, 200519), generating plasmid pAV008 containing dcp2-E149Q,E153Q ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoter Capture was carried out using Hi-C libraries from three Vehicle-treated tumour samples and three Decitabine-treated tumour samples with the SureSelect target enrichment system and RNA bait library according to manufacturer’s instructions (Agilent Technologies kit), using 12 post-capture PCR cycles as required ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The L834R mutation was introduced in EGFR using the QuikChange II Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Agilent Technologies, #200523). The plasmid used for the neural network training encoded for the extracellular and transmembrane domain (ECTM ...
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bioRxiv - Microbiology 2022Quote: ... C194 in NlpC/P60Ela and C153 in in NlpC/P60Sne were changed to alanine using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). The respective sense and antisense primers listed in Supplementary Table 1 were used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using the broad range RNA assay and rudimentary cDNA quality and size information was obtained from an Agilent 2100 Bioanalyzer with a Broad Range DNA Kit (Agilent, USA). For Qubit conversions from ug to picomoles cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA isolated from jejunum was subjected to quality control analysis using an Agilent 2100 Bioanalyzer with RNA 6000 Nano Kits (Agilent, USA). After poly A enrichment the samples were fragmented and reverse-transcribed to generate complementary DNA for sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Required quality control steps and quantification measurements within this protocol were performed using the Agilent High Sensitivity DNA Kit (5067-4626, Agilent Technologies) and the KAPA Library Quantification Kit (2700098952 ...
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bioRxiv - Cell Biology 2023Quote: ... Probes (available in Table S7) were labeled with 32P-dCTP using the Prime-It RT random labeling kit (Agilent, catalog #300329) and hybridized to the membrane ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids harboring GlnR amino acid substitutions (pET28a-glnR derivatives) were constructed using site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit, Agilent, Inc.).DNA fragments consisting of different canonical GlnR-dependent promoters (narG whose downstream genes encoding M ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates from at least 3 independent biological samples using the SYBR green Brilliant II fast kit (Agilent Technologies) on an Mx3005p apparatus (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The T94D mutation was introduced in the plasmids by PCR using the QuickChange Multi-Site Directed Mutagenesis kit (#200514 -Agilent Technologies). The sequence of the primer used to introduce the mutation T94D in the Nme1 sequence of plasmids is reported in key resources table ...
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bioRxiv - Cell Biology 2023Quote: ... keeping in parity the Y320 residue of Gαi3 and Gαs the Y320E and Y320F mutants were generated using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The mutagenesis of the HDE-like motifs and PAM sequence within donor plasmid for HDR was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). pcDNA3.1(+)-ARRDC4-1 and pcDNA3.1(+)-ADCYAP1-2 expression vectors were constructed by cloning lnc-ARRDC4-1 and lnc-ADCYAP1-2 sequences to pcDNA3.1(+ ...
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bioRxiv - Synthetic Biology 2023Quote: ... The average length of the DNA libraries was evaluated using Agilent Bioanalyzer 2100 and a High Sensitivity Kit (Agilent Technologies, USA) and the concentration of the DNA libraries was determined with a Qubit device (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies, Cat. No. / ID: 5067-4626). Libraries were sequenced on an Illumina NextSeq2000 in paired end mode with the read length of 50 nt and sequencing depth of 25 million reads per library ...
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bioRxiv - Genomics 2023Quote: We purified the DNA using AMPure beads (Beckmann Coulter, USA) and assessed quality using a Large Fragment Analysis Kit (Agilent, USA). The library was prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Systems Biology 2023Quote: ... The quality and quantity of all libraries were checked using Agilent’s Bioanalyzer 2100 and DNA 7500 kit (Agilent Technologies, 5067-1506).
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bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
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bioRxiv - Molecular Biology 2023Quote: ... The sequence was mutagenized to obtain the N-ter tag canonical variant 2 through the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521) using the following DNA primers ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality of RNA was evaluated using the Agilent 2100 Bioanalyzer with Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511). Libraries were prepared using the Illumina TruSeq Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina according to the manufacturer’s protocol and sequenced on an Illumina HiSeq 4000 (2× 75 bp).