Labshake search
Citations for Agilent :
6501 - 6550 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Sizes of indexed amplicons were verified using the High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer system (Agilent Technologies, Canada). Indexed amplicons were pooled in equimolar amounts and sequenced on an in-house Illumina MiSeq benchtop sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA (100 ng) was labelled with Cy5 or Cy3 using the Two-Color Low Input Quick Amp labelling Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was next converted to cDNA by using a polydT primer and following the AffinityScript Multiple Temperature cDNA Synthesis kit instructions (Agilent Technologies). In order to quantify the HEV genome ...
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bioRxiv - Immunology 2019Quote: ... Fragment sizes were confirmed using either the High Sensitivity DNA assay or the DNA 1000 kit and 2100 bioanalyzer (Agilent Technologies). Libraries were quantified with qPCR ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were then validated on a Fragment Analyzer system with a High Sensitivity NGS Fragment Kit (Agilent Technologies, Mississauga, ON, Canada) to check for size and quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... The SfiI site in mouse Tns4 cDNA was mutated using the QuikChange II XL mutagenesis kit (Agilent, Santa Clara, CA, USA) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... or turboGFP-MCMBPΔNLS (bipartite NLS was identified using cNLS Mapper35 generated by site directed QuickChange II XL Site-Directed Mutagenesis kit (Agilent, 200522) with primers (forward ...
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bioRxiv - Neuroscience 2020Quote: ... The integrity of the RNA was checked by capillary electrophoresis using the RNA 6000 Pico Labchip kit and the Bioanalyser 2100 (Agilent Technologies), and quantity was estimated using a Nanodrop 1000 (ThermoScientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... staggered QuikChange oligos AG66/AG67 were used to generate the A783U mutation in pTRE3G-HOTAIR using the QuikChange Site Directed Mutagenesis Kit (Agilent 200519) to generate pTRE3G-A783U_HOTAIR ...
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bioRxiv - Microbiology 2019Quote: ... A site directed mutagenesis of the cloned UBP-1 PCR product in the TOPO 2.1 vector was carried out using a QuikChange® multi-site directed mutagenesis kit (Agilent technologies) using the following primer combinations ...
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bioRxiv - Microbiology 2019Quote: ... Both infected and mock-infected RNA samples were labeled with Cy3 and Cy5 using an Agilent low-input RNA labeling kit (Agilent Technologies). Cy5-labeled infected or mock-infected samples were co-hybridized with Cy3-labeled mock-infected or infected samples in one array ...
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bioRxiv - Microbiology 2020Quote: ... we carried out site direct mutagenesis of pCR-ompT-GFP according to the Quikchange II site-directed mutagenesis kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were quantified with Qubit™ dsDNA high sensitivity assay kit (Thermo) and further analyzed with the high sensitivity D1000 ScreenTape system (Agilent). Sequencing was performed on a NextSeq 500 with onboard clustering using a 75-cycle high output v2.5 kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and small RNA from BH were analyzed using capillary electrophoresis by RNA 6000 Nano Kit (Agilent Technologies 5067-1511) and RNA 6000 Pico Kit.
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bioRxiv - Biophysics 2021Quote: ... and E217A were cloned with primers IF733 and IF734 using QuikChange Lightning Multi Site-Directed Mutagenesis Kit to generate plasmid pIF585 (Agilent #210516). RAD51(K133R ...
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bioRxiv - Biophysics 2021Quote: The mutant A350P mutant was generated using pET-LA as template by site directed mutagenesis (QuickChange II XL Site Directed Mutagenesis Kit, Agilent-USA). The primers used for the same were a ...
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bioRxiv - Biophysics 2020Quote: ... The desired base pairs coding for Cys were introduced using overlapping primers with the QuikChange II site-directed mutagenesis kit (Agilent Technologies) and were confirmed by DNA sequencing (GENEWIZ ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 independent batches of RNA were prepared and controlled for purity and integrity using the Agilent 2100 Bioanalyzer with RNA 6000 Nano LabChip kits (Agilent Technologies). Only RNA with no sign of contamination or degradation (RIN > 9 ...
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bioRxiv - Neuroscience 2022Quote: Analysis of mitochondrial respiration was performed using Seahorse XFp Flux analyzer with a Seahorse XFp Mito Stress Test Kit (Agilent Technologies) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid pEYFP-NONO containing siRNA resistance site corresponding to siRNA NONO s9612 was made by QuikChange site-directed mutagenesis kit (Stratagene, 200518) with base-pair substitutions and the template plasmid pEYFP-C1-NONO (human) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting eight vectors were then subjected to site-directed mutagenesis (QuickChange II XL Kit; Agilent Technologies, La Jolla, CA, USA) to introduce the codons of interest ...
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bioRxiv - Cancer Biology 2022Quote: ... ECAR (mpH/min) and OCR (pmol/min) values were obtained using Seahorse XFp Cell Energy Phenotype Test Kit (Seahorse Bioscience Inc.). Experiments were performed in triplicate and repeated at least three times.
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bioRxiv - Neuroscience 2022Quote: ... CAS No 1582-09-8) was accessed using the Seahorse XF 96 Extracellular Flux Analyzer and the XF Cell Mito stress test kit (Agilent Technologies) following Agilent Technologies guidelines ...
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bioRxiv - Immunology 2022Quote: ... A second reverse transcription reaction was performed with MARS-seq RT2 primer and the Affinity Script cDNA Synthesis Kit (Agilent Technologies) followed by 1.5X AMPure XP bead cleanup ...
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bioRxiv - Molecular Biology 2022Quote: ... E197A and D199A were introduced in the parental pcDNA5/FRT/TO-DDX39B plasmid (Galarza- Munoz et al., 2017) using QuickChange Lightning Mutagenesis Kit (Agilent Technologies). All constructs and mutations were confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2022Quote: RNA-sequencing libraries were prepared using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing kit (Agilent, Cat#: G9691A) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli and used as a template for generation of E2 mutants by a QuikChange Lightning Site Directed Mutagenesis Kit (Agilent Technology) following the supplier’s procedure ...
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bioRxiv - Biochemistry 2022Quote: ... and C507A CAPN5 mutations and the C506A CAPN6 mutation were introduced with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The C246A and C512A mutations and the C495A/C507A/C512A triple mutation were introduced by PCR amplification of phCAPN5-3×FLAG with 5’-phosphorylated primers incorporating the respective mutations ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for mycoplasma at the beginning of the studies (tested negative with MycoSensor PCR Assay Kit, Agilent, #302109) and have never exhibited contamination symptoms after initial testing.
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bioRxiv - Cancer Biology 2022Quote: ... Point mutants for ENDOD1 and TP53 were converted from parental pLVX-Flag -IRES-ZsGreen1 plasmids using QuikChange Lightning Site-Directed Mutagenesis Kits (Stratagene, 200519). Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... a stop codon was introduced into a previously generated pDONR-CP110L (containing a full length CP110 cDNA) (Franz et al., 2013) by site directed mutagenesis using Quikchange II XL mutagenesis kit (Agilent technologies). This was then recombined with the pUbq-empty vector (This paper ...
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bioRxiv - Biochemistry 2022Quote: ... The D454N mutation was introduced by site-directed mutagenesis using the QuickChange™ II XL site-directed mutagenesis kit (Agilent technologies). An overlap extension PCR strategy was used to insert the 3C protease site (LEVLFQGP ...
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bioRxiv - Cancer Biology 2022Quote: ... Sirt1 H355A mutation was generated by performing directed mutagenesis on this vector using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... C278 and C830 (as determined by GPS-SNO 1.0 software with high threshold) using Quick Change Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, USA). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... CD58K34A OE plasmids were generated through site-directed mutagenesis of existing CD58 ORF plasmids using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies) per manufacturer protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Biochemistry 2020Quote: ... SDM was carried out with appropriate primers (Table 4) using QuikChange® Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for RNA sample preparations ...
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bioRxiv - Immunology 2021Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog number 210518, Agilent Technologies). The mutation was confirmed by full-length spike gene sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 96 libraries were generated and their profiles were analyzed using the BioAnalyzer 2100 with a High-Sensitivity DNA kit (Agilent, USA). To ensure the evenness of library pooling ...
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bioRxiv - Microbiology 2021Quote: ... The enzymatically-inactive pCAGGS-TMPRSS2(S441A)FLAG mutant cDNA was generated using QuickChange Site-Directed Mutagenesis Kit per manufacturer instructions (Agilent Technologies). Transient transfections of HEK-293T cells were performed using PolyFect transfection reagent per manufacturer instructions (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis for Cys238>Ala active site substitution in scpA was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using oligonucleotides MP_scpA1 and MP_scpA2 [49].
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bioRxiv - Neuroscience 2020Quote: ... The RNA was quantified with a Nanodrop 1000 spectrophotometer and its integrity checked with the Bionalyzer (Agilent RNA 6000 nano kit). RT-qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... RNA concentration and purity were assessed using NanoDrop ND-1000 spectral photometer (Peqlab) and the quality of the RNA was determined using RNA 6000 Nano LabChip Kits (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA integrity was assessed with the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Waldbronn, Germany) and the RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2021Quote: ... they were prepared and assayed for mitochondrial function assessment according to manufacturer instructions (Seahorse XF Cell Mito Stress Test Kit, Agilent Technologies). More details are found in Supplemental Methods.
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bioRxiv - Microbiology 2021Quote: ... The RNA integrity number and library insert size were verified using the Agilent RNA 6000 Pico Kit and Bioanalyzer platform (Agilent, USA). The RiboZero (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... encoding the SAMHD1 mutant NLS (mNLS) and the P275A mutants were generated using a QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol using the following primers ...