Labshake search
Citations for Agilent :
6451 - 6500 of 8606 citations for Estriol ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: All TTBK2 mutations were generated by PCR-based site-directed mutagenesis by using QuickChange Lighting Site-Directed-Mutagenesis kit (Agilent) using as a template pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA libraries were finally assessed for quality with the Agilent 2100 BioAnalyzer using DNA high sensitivity kit (Agilent: 5067-4626) and quantified using Qubit DNA high sensitivity assay (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2023Quote: ... RNAseq analysis was performed by LC Sciences (Houston, TX) for RNA purity/quantity assessment via Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent), mRNA extraction ...
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bioRxiv - Genetics 2023Quote: ... The final libraries were quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent, 5067-4626), Qubit dsDNA HS Assay kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... The RNA quality was validated using NanoDrop and Agilent DNF-472 HS RNA (15 nt) Kit (Agilent Technologies, CA, USA) on the 5200 Agilent system to confirm the purity ...
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bioRxiv - Genomics 2023Quote: ... Purified RNAs were quantified by Qubit and quality was assessed using RNA Pico Assay kit with Bioanalyzer (Agilent Technologies, USA). chrRNA were then subjected to library preparation using True-seq stranded total RNA library prep gold kit (Ref#220599 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... An A to C mutation was made at base pair 219 within the CAT-I gene using the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent) to match the native E ...
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bioRxiv - Biophysics 2023Quote: ... A clone encoding tagless wild type PUS1 was then generated from PUS1D134A by converting alanine 134 back to wild type aspartate 134 using a QuickChange II XL kit and protocol (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The mRNA was purified and fragmented from a total amount of 1 μg RNA per sample ...
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bioRxiv - Cancer Biology 2022Quote: Percent ATP produced by mitochondria and the level of compensatory glycolysis were measured using a Seahorse XF Real-Time ATP Rate Assay Kit and XFe96 Analyzers (Agilent) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA yield was measured with a Nanodrop and the RNA integrity values (RIN) were obtained with a Bioanalyzer 2100 (Pico RNA 6000 Kit, Agilent). The values obtained for each of the samples are shown in Supplementary Table S1.
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bioRxiv - Immunology 2023Quote: ... following the manufacturer’s instructions and were run on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit (Agilent Technologies) to ensure the proper range of cDNA length distribution ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg DNase-treated RNA was reverse transcribed using a cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), following the respective manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genetics 2023Quote: ... Point mutations in KCNJ1 were introduced into the resulting pRS415TEF1-ROMK1 vector using either two-step overlap extension mutagenesis (141) or site-directed mutagenesis with the QuikChange kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene, CA) (Yoshida et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... according to manufacturer’s instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA-enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... The mutagenic primers were designed using QuickChange primer design program at www.agilent.com/genomics/qcpd according to the guidelines mentioned in the manufacturer’s protocol (QuickChange Lightning Site-Directed Mutagenesis kit, Cat# 210518, Agilent Technologies). This was followed by mutant strand synthesis and transformation was performed as mentioned in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 50 μM 2-deoxy-glucose (2-DG) + 1μg/μL Hoechst (Seahorse XF Glycolysis Stress Test Kit, Agilent, 103020) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration and quality of the synthesized MPXV mRNA were measured using an Agilent 2100 Bioanalyzer and RNA Nano 6000 Assay Kit (Agilent), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... DNA integrity was evaluated with a Fragment Analyzer (Advanced Analytical Technologies) using the HS NGS Fragment Kit (DNF-467, Agilent). dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... UHOR_02675 active site mutants (Ribo1-ME80Q/D98N) were created from by using QuickChange XL Site-Directed Mutagenesis Kit from Agilent Technology according to manufactures instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and run on the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara CA, USA) to confirm amplicon length and purity ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA concentration and quality of the RNAseq libraries were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent), and libraries were sequenced as single-end 50 bp reads on an Illumina HiSeq 2500.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with either EGFP-AKT2 or EGFP-AKT2+ HA-SIRT5 constructs or left untransfected and after 48 hours were subjected to the Mitostress assay kit (Cat# 103015-100) from Agilent, USA28.
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bioRxiv - Neuroscience 2023Quote: ... Generation of GluK2 point mutants was performed by site-directed mutagenesis using the QuickChange Mutagenesis Kit (Stratagene, La Jolla, CA). Forward and reverse primer for creation of mutants were ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 the XF Real-Time ATP Rate Assay Kit (Cat. No. 103592-100, Agilent Technologies, Cedar Creek, TX) was run following the manufacturer’s protocol using the Seahorse XF96 Analyzer as described above.
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bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and the RNA integrity number (RIN) measured using the standard Agilent RNA 6000 Nano Kit Protocol and BioAnalyzer (Agilent Technologies). Extractions <7 RIN were not sequenced ...
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bioRxiv - Genetics 2023Quote: ... PREPLs was cloned into the bacterial expression vector pVP68K and mutations were introduced using the QuikChange II site directed mutagenesis kit (Stratagene) and confirmed by direct Sanger sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... The library size distribution was determined using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit (5067-4626, Agilent). We next used the SureSelect XT HS reagent Kits (5190-9685 and 5191-6686 ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned up using the Takara Nucleospin kit per manufacturers protocol and concentration and size range assessed using the D5000 Screentape (Agilent). 200fmol of each sample was barcoded and subject to library prep using the Native Barcoding Ligation Kit with V14 chemistry (SQK-NBD-114-24 ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using the NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified with the Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated by RNeasy Mini or RNeasy Plus Micro Kit and controlled for quality with a 2100 Bioanalyzer (Agilent) prior to cDNA libraries construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
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bioRxiv - Neuroscience 2023Quote: ... Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit; Quiagen; 204145) performed with the MX3005P thermocycler (Stratagene).
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Genomics 2023Quote: ... the concentration and integrity of the RNA were assessed using the ND-1000 UV-visible light spectrophotometer from Nanodrop Technologies and the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit by Agilent.
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bioRxiv - Genetics 2023Quote: ... The quality and concentration of the library were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... The integrity of the RNA sample was verified using the Agilent RNA 6000 kit and Agilent Bioanalyzer 2100 (Agilent Technologies). Samples with an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2023Quote: ... Specific nucleotide substitutions or coding-sequence truncations were introduced as required using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent). All plasmid inserts and modifications were authenticated by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... and resulting size distribution was assessed with the High Sensitivity (HS) Large fragment kit run on the Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The integrity of the RNA was tested on a Bioanalyzer 2100 using the Prokaryote Total RNA Nano kit (Agilent Technologies) and RNA Integrity Numbers (RIN ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were analysed on the 2100 Bioanalyzer Agilent system using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626). DNA concentrations were measured by Qubit Quant IT kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified pools were quantified using Qubit dsDNA HS Assay and the quality checked using a Tapestation High Sensitivity D1000 kit (Agilent) to ensure the size distribution of amplicons was as expected ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was quantified by NanoDrop and loaded abundance measured using a Pico 6000 kit and Eukaryotic Total RNA assay on a BioAnalyzer (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... The size distribution and quality of the libraries were evaluated using a high sensitivity DNA kit (Agilent Technologies #5067-4626). Quantification of libraries was carried out with the Quant-iT DNA Assay Kit ...