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Citations for Agilent :
6301 - 6350 of 8653 citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The specific variants were generated by site-directed mutagenesis in pCMV6-Entry-YTHDF2 using the QuikChange II site-Directed Mutagenesis Kit (Cat# 200521, Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and Halo-V5-WTAP mutants (D301A and D301A/D302A) were generated using the QuikChange II site-directed Mutagenesis Kit (Stratagene) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PartB RNA was quantified and purity/quality was assessed on the 2100 Agilent BioAnlayzer using the Agilent small RNA kit (5067-1548, Agilent).
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bioRxiv - Genetics 2020Quote: ... The A>T variant was introduced to PIK3C2B-pNL construct by site-directed mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) to generate PIK3C2B-A>T-pNL construct ...
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bioRxiv - Immunology 2019Quote: ... cDNA synthesis was performed using AffinityScript QPCR cDNA Synthesis kit following the manufacturer’s guidelines (Agilent Technologies, Santa Clara, CA, USA). The total reaction volume was 10 µL using 5 µL of Mastermix ...
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bioRxiv - Immunology 2019Quote: ... C-terminally HA-tagged wt IFITM3 genes in the lentiviral vector pHR-SIN cGGW PGK puro were mutagenized using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions using the following primers (all listed 5’ to 3’) ...
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bioRxiv - Biochemistry 2019Quote: ... Mutagenesis was performed with pHmtS-ET22b as a template using a QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Site-specific mutations of ACC2 and stop codon deletion constructs were performed using PCR-based mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent). The plasmids for C-terminus GST-tagged or Flag-tagged ACC2 fragments were re-cloned from the human ACC2 plasmid using PCR and Gibson assembly ...
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bioRxiv - Microbiology 2019Quote: ... All GBF1 truncations were introduced into the backbone of Venus tagged-GBF1/A795E (described in 57) using QuickChange XL site-directed mutagenesis kit from Agilent Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... was created by introducing the point mutation in wild-type vasa cDNA sequence by site directed mutagenesis using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The transgene is subsequently cloned downstream of GFP into the pUASK10attB vector (gift of Beat Suter) ...
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bioRxiv - Molecular Biology 2019Quote: ... Csl4-F121A and Csl4-F129A variants were generated by site-directed mutagenesis of their wildtype counterparts with appropriate sets of oligonucleotides using the QuikChange II XL Kit (Stratagene) as recommended.
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bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... RNA size and integrity were confirmed using RNA 6000 Pico kit for the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and Nanodrop™ ND-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Genetics 2019Quote: ... Exons of DNA samples were captured using the in-solution enrichment methodology (Agilent SureSelect All Exon XT2 50 Mb Kit) and sequenced with an Illumina HiSeq 2500 instrument to generate 100 bp paired-end reads ...
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bioRxiv - Microbiology 2019Quote: ... Mutation of glycine at position 2 to alanine was accomplished using the Quick-Change Mutagenesis kit (Agilent, Santa Clara, CA) to generate ptubTgFBXO1HAG2A.
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bioRxiv - Genetics 2020Quote: ... WGS library-size selection was then verified using the 2100 Bioanalyzer DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA). The WGS library was then sequenced (2×150 bp ...
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bioRxiv - Genetics 2020Quote: ... in the nmt1-GFP-swi6+ expression construct (kindly gifted by Dr. Alison Pidoux) using the QuickChange site-directed mutagenesis kit (Stratagene), as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... plasmid using oligonucleotides containing the SHRF syndrome-linked G58V and G226D missense mutations and the QuikChange Site-Directed Mutagenesis Kit (Stratagene). The untagged RRP4/rrp4-G226D (pAC3656 ...
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bioRxiv - Genetics 2020Quote: The BRCA2 and PALB2 constructs were made siRNA resistant by introducing three silent mutations with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed mutagenesis of Aγ was performed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent, Santa Clara, CA). cRNAs were transcribed using mMessage mMachine Transcription Kits (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... total leukocyte DNA was enriched using the Agilent SureSelect Human All Exon 50 Mb capture kit (Agilent Technologies, SantaClara, CA). Bidirectional 100-bp paired-end sequencing (Illumina Hi-Seq 2000 ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Genomics 2021Quote: ... Concentration and quality of the libraries were measured using Qubit® 2.0 Fluorometer and Bioanalyzer High Sensitivity DNA Analysis kit (Agilent), respectively ...
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bioRxiv - Plant Biology 2021Quote: ... The total RNA quality was assayed using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies, St. Clara, USA). Labelling was done following the Low Input Quick Amp Labelling protocol of the Two-Color Microarray-Based Gene Expression Analysis system (Agilent Technologies ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: The first yeast library (called library A in this study) of FAST constructed by error-prone PCR using Genemorph II kit (Agilent) was previously described23 ...
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bioRxiv - Neuroscience 2021Quote: ... Both Southern blot probes were generated by standard PCR and subjected to random labelling using a Prime-It II Random Primer Labelling Kit (#300385, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA integrity was determined by microfluidic capillary electrophoresis using the Agilent RNA 6000 Nano Kit and the 2100 Bioanalyzer Instrument (Agilent).
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bioRxiv - Molecular Biology 2020Quote: ... Mutation of the near-cognate codon AAG to CAG was performed using a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All generated constructs were verified by Sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... and RNA integrity was assessed using the RNA Nano 6000 Assay Kit for the Agilent Bioanalyzer 2100 system (Agilent Technologies). Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted RNA using the Qiagen RNeasy Blood and Tissue Kit and checked RNA integrity with a Bioanalyzer 2000 (Agilent) or TapeStation 2200 (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed using Illumina Nexterna XT kit and were analyzed for concentration by Qubit and for size distribution by Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2019Quote: ... RNA integrity was measured using Agilent 2100 Bioanalyzer System and Agilent RNA 6000 Pico Kit (Cat. No. 5067-1513, Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The D238A catalytically deficient fbl mutant was generated by site-directed mutagenesis with the help of the QuikChange II site-directed mutagenesis kit (Stratagene) and primers LD3881 and LD3882 ...
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (medaka hybrid and mCherry or KDM4E-injected zebrafish samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: The pGEX6P1-Hecw C1394W construct was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit protocol (Agilent) using the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... 67)) plasmid as PCR template Bri2 BRICHOS D148N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Cancer Biology 2021Quote: ... The amount and average fragment length of amplified RNA was determined using the RNA 6000 Pico Kit (Agilent, 5067-1513) with a 2100 Bioanalyzer according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: Mutations were introduced to LRH-1 in the pMSC7 and pCI vectors using the Quikchange Lightning Site-Directed Mutagenesis Kit as directed by the manufacturer (Agilent). Constructs were sequenced prior to use.
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... MYC-binding sites (E-boxes with the sequence CACGTG at positions -1149 bp and -1353 bp upstream of the transcription start site of the PTEN gene) were mutated to CAAGAA using Quikchange Lightening kit (Agilent). The TATA synthetic promoter sequence was derived from pGL firefly reporter with a minimal promoter.
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bioRxiv - Biochemistry 2020Quote: ... This construct was expressed in the HEK cell line and regenerated with 11-cis-retinal.22 MRh was prepared similarly.22 Site-directed mutagenesis was performed using the QuikChange Multisite-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... DNA quality of the samples was assessed with the Fragment AnalyzerTM and the DNF-488 High Sensitivity genomic DNA Analysis Kit (Agilent). DNA was slightly more fragmented than defined by the quality control standards but this fragmentation was minor ...
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... psub-201 [61] containing the full-length AAV2 genome as template and the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.