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Citations for Agilent :
6151 - 6200 of 6515 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Probes produced from PCR templates were radiolabelled with [α-32P]-dCTP (Hartmann Analytic, #SRP-305) using Prime-it II Random Primer Labelling kit (Agilent, #300385) and oligonucleotides used as a probe were radiolabelled with [γ-32P]-ATP (Hartmann analytic #SRP-501) ...
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bioRxiv - Developmental Biology 2024Quote: ... Exome capture was performed using the IDT xGen Exome Research Panel v2.0 in conjunction with library preparation using the SureSelectXT kit (Agilent Technologies, California, USA) following the manufacturer’s recommendations and incorporating Illumina® 8-nt dual-indices ...
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bioRxiv - Physiology 2024Quote: cDNA encoding the mouse γ subunit was mutated to replace 140RKRR143 with 140QQQQ143 using the QuckChange II XL mutagenesis kit (Agilent Technologies). cDNAs encoding ENaC’s mouse α ...
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bioRxiv - Immunology 2023Quote: ... Mutations were introduced into cDNAs using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA, USA; cat. no. 200521) to construct expression vectors for S variants with a single amino acid change ...
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bioRxiv - Physiology 2024Quote: ... Library quality assessment was performed twice during the library generation protocol with the Agilent Bioanalyzer High Sensitivity dsDNA kit (Agilent; 5067-4626). Completed libraries were quantified with both the Qubit high sensitivity dsDNA kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2024Quote: ... sgRNA-encoding genomic loci were amplified from up to 400 µg gDNA via PCR using a Herculase II Fusion Polymerase PCR kit (Agilent, Cat. #600677). Pooled reaction products from each treatment group were uniquely barcoded in a subsequent PCR reaction followed by electrophoresis on 2% TBE-agarose gel at 120 V ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragment size of the libraries was assessed using the Agilent Bioanalyzer 2100 with the High Sensitivity DNA kit (Agilent, 5067-4626). The concentration of the libraries was measured by the High Sensitivity QuBit kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Final sequencing libraries were quantified using a Qbit DNA HS kit (Thermo #Q32856) for concentration and a Bioanalayzer HS DNA chip (Agilent #5067-4626) for amplicon size ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was checked pre and post poly(A)tail addition using an Agilent RNA 6000 Nano kit run on a 2100 BioAnalyser (Agilent Technologies, USA) (Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA quality was determined by measuring the RNA integrity number (RIN) via the High Sensitive RNA Screen Tape Analysis kit (Agilent Technologies #5067) on the TapeStation system (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: All mutants were generated by site-directed mutagenesis with the QuikChange XL II site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA), as previously described (33).
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bioRxiv - Cancer Biology 2024Quote: ... Drug was then washed out and 1x105 cells plated in 5 replicate wells for immediate analysis using the Mito Stress Test Kit (Agilent, 103015-100) according to the manufacturer’s instructions and using the following drug concentrations ...
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bioRxiv - Cell Biology 2023Quote: Measurements of aerobic respiration and glycolysis were conducted with the Seahorse Bioscience XFe96 bioanalyzer using the seahorse XF Mito Stress Test Kit (Agilent #103015-100) and the Seahorse XF Real-Time ATP Rate Assay Kit (Agilent #103592-100) ...
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bioRxiv - Cell Biology 2023Quote: ... GYF motif mutant GIGYF1 (GIGYF1 GYF Mut; G502A, Y503A, F504A) plasmids were created using the QuikChange II Site-Directed mutagenesis kit (Agilent Technologies, 200523). The chimeric constructs were synthesized by GenScript (Piscataway ...
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bioRxiv - Neuroscience 2023Quote: ... targeted mutagenesis was performed on the cloned WT p38γ by using the Quick-Change Lightning site-directed mutagenesis kit (Agilent Technologies; 210518) to create the kinase dead mutant.
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bioRxiv - Genetics 2023Quote: Exome enrichment was applied to the cDNA purified from the previous step by using probe kit SSELXT Human All Exon V8 (Agilent, 5191-6879) for human samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNANano 6000Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Three independent biological replicates were performed ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... the A3 sequence specifying amino acids 21-393 was amplified from pHD1344tub(PAC)-Nterm3V5-A3 by error prone PCR using the GeneMorphII Random Mutagenesis kit (Agilent, Cat # 200523) and primers that add AttB sites (Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis was performed on the pGAMA-YAP construct to create p-GAMA-YAP-S127A using the QuikChange Lightning kit (Agilent Technologies #210513). Forward primers used for the Ser-to-Ala substitution was as follows ...
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bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2023Quote: ... RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer kit for total eukaryotic RNA (Agilent Technologies, Santa Clara, CA, USA). Samples underwent library preparation and sequencing at the UC Berkeley Functional Genomics and Vincent J ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control of the final libraries was completed on the Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent, 5067-4626) to determine the average library sizes ...
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bioRxiv - Cell Biology 2023Quote: ... Most missense and deletion mutations were inserted by site-directed mutagenesis using the QuikChange Lightning Kit (Agilent Technologies, Santa Clara, CA, USA) in combination with appropriate oligonucleotides (Supplements ...
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bioRxiv - Neuroscience 2023Quote: ... The quality of the extracted RNA was assessed by the 2100 Bioanalyzer Instrument using Agilent RNA 6000 Pico kit (Agilent, CA, USA) and an average integrity quality number (RIN ...
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bioRxiv - Genomics 2023Quote: ... Appropriate fragment size distributions were confirmed by running each sample on an Agilent Bioanalyzer using the DNA High-Sensitivity Kit (Agilent, #5067-4626).
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bioRxiv - Immunology 2023Quote: ... and size distribution was assessed using the Agilent High Sensitivity DNA Kit on the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Sequencing was performed using an Ion 540™ chip and the 540™ Chef kit on an Ion GeneStudio S5 sequencer.
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration and the RNA integrity number (RIN) were determined using a Bioanalyzer 2100 instrument and an Agilent RNA 6000 Pico Kit (Agilent 5067-1513).
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bioRxiv - Biophysics 2023Quote: ... the following HIV-1 IN mutants were prepared using the QuikChange II XL site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA): E138K ...
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Genetics 2023Quote: ... and Illumina adapters were added (second PCR) in a nested PCR manner using Agilent Herculase II Fusion DNA Polymerase Kit (Agilent, Cat # 600679). The PCR products were purified and sequenced using an Illumina NextSeq ...
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Genomics 2023Quote: ... All library validations were performed on a 2100 Bioanalyzer with High Sensitivity DNA kit according to manufacturer’s recommendations (Agilent Technologies, 5067-4626).
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was determined with an RNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5576, 5067-5578, 5067-5577) and only RIN scores of 8 or higher were used for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... A sequence coding for the MAAAPQKC peptide was inserted in place of the first ten codons of the ermCL sequence using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Inc). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutants of KflaHY2 were generated in the pET28a_KflaHY2 plasmid using the QuikChange® Lightning kit (Agilent, Santa Clara, California, USA) employing primers listed in Table S1 ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Genomics 2023Quote: ... cDNA was diluted to approximately 3 ng/μL and run on the 2100 Agilent Bioanalyzer using the High Sensitivity DNA Kit (Agilent 5067-4626). The measured fluorescence units in Fig 1b have been corrected for the dilution factor.
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bioRxiv - Plant Biology 2023Quote: ... Site directed mutagenesis was performed using the QuikChange Site-directed Mutagenesis Kit following the instructions of the manufacturer (Stratagene; La Jolla, CA) using the primers listed in Supplemental Table 1.
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bioRxiv - Neuroscience 2023Quote: ... were used for each replicate and placed into 100 µL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA quality was evaluated using the 5200 Fragment Analyzer System and the Agilent HS RNA Kit (Agilent Technologies, Inc., CA, USA). After the ribosomal RNA depletion with the RiboPOOL kit (siTOOLs Biotech GmbH ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Microbiology 2023Quote: ... and their concentrations were checked by Qubit (ThermoFischer Scientific) followed by quality assessment through Agilent 2100 BioAnalyzer (Agilent RNA 6000 Nano Kit). Both sets of RNA seq represented in Figures 1 and 5 were processed and run at the same time ...
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bioRxiv - Systems Biology 2023Quote: ... and confirmed the quality of the RNA samples using a Fragment Analyzer (Advanced Analytical Technologies, Inc.) with the Standard Sensitivity RNA Analysis Kit (Agilent, #DNF-471).
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bioRxiv - Neuroscience 2023Quote: ... Total RNA of 100ng was subjected to reverse transcription using the AffinityScript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A 2 µl aliquot of processed gDNA was taken to assess shearing using an Agilent High Sensitivity D1000 Kit (Agilent, Cat. #G2991AA). Once shearing was assessed ...
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bioRxiv - Molecular Biology 2023Quote: ... and the size distribution of the library was assessed using an Agilent 2100 Bioanalyzer with a High-Sensitivity DNA analysis kit (Agilent, 5067-4626). DETECTOR-seq libraries were sequenced on an Illumina HiSeqX platform to a depth of 10 million 150 bp paired-end reads per sample ...
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bioRxiv - Immunology 2023Quote: ... Pool quality control was performed with an Agilent High Sensitivity D1000 Screen Tape reagent kit using the Agilent 4200 TapeStation (Agilent Technologies, USA). Whole-genome sequencing (WGS ...
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bioRxiv - Cell Biology 2023Quote: pLV-CMV-IRIS-PURO-c-Src-mScarlet plasmid was used to the generation of Y527F and Y527F/K295R mutants via QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200523) according to manufacturer’s protocol using the primers listed in Table 1.