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Citations for Agilent :
551 - 600 of 2367 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were blocked with DAKO Protein Block Serum-Free (DAKO, Catalog No. X0909) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... and manufacturer’s instructions followed for protein binding and elution (Agilent, Santa Clara, CA). The low abundance protein fraction was further fractionated by 1D SDS PAGE and analyzed using LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Digested protein (100μg) was loaded using high-performance liquid chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Molecular Biology 2020Quote: ... 200µg digested protein was loaded using high Performance Liquid Chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Molecular Biology 2020Quote: ... After treatment with a Protein Block Serum-Free blocking reagent (DAKO, Code X0909) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue sections were then blocked with serum-free protein blocking agent (Dako) and incubated with rabbit mAb for Phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-Glial fibrillary acidic protein (GFAP; 1:1000, Dako, Cat# Z033429, RRID:AB_10013382), mouse anti-CD68 (ED1 ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilised (0.1% TritonX-100 in PBS) and blocked (Serum free protein block, DAKO), before overnight incubation with a rabbit polyclonal antibody to mouse CD31 (ab28364 ...
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bioRxiv - Neuroscience 2021Quote: ... Target protein was further purified by a 300SB-C3 RP-HPLC column (Agilent) with a linear gradient of acetonitrile (0-100% ...
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bioRxiv - Microbiology 2021Quote: ... For digestion of proteins in the insoluble fraction 20 µl StrataClean beads (Agilent), which bound 20 µg protein ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Neuroscience 2022Quote: ... Ki67 (1:1000) and glial fibrillary acidic protein (GFAP) (1:1500) from Dako Cytomation (Glostrup ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm.
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bioRxiv - Neuroscience 2024Quote: ... Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako, Denmark, Z0334, dilution 1:200), Rabbit Anti-Iba1(Fujifilm ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... they were exposed to Protein Block Serum-Free Ready-To-Use (DAKO, Denmark) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Protein unfolding profiles were recorded using an MX3005P real-time qPCR instrument (Agilent; excitation/emission filters = 492/610 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Endogenous peroxidases and non-specific signal (Flex Peroxidase Block and Protein Block, Dako) were blocked at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: Urea in-solution protein digestion was performed on the AssayMAP Bravo (Agilent Technologies) micro-chromatography platform using the digestion v2.0 protocol ...
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bioRxiv - Physiology 2023Quote: ... blocked with a serum-free Protein blocking solution (DAKO, Los Altos, CA, USA) for 30 minutes and rinsed three times with PBST solution ...
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bioRxiv - Biophysics 2024Quote: Protein variants were constructed using a QuikChange site-directed mutagenesis kit (Agilent, USA). The primer that introduced desired nucleotide changes was designed using QuikChange online tool (Table S3C ...
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bioRxiv - Physiology 2024Quote: ... slides were rinsed in PBS and blocked with Protein block serum-free (Dako) for 1 hour at r/t or overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were separated by the in-line SEC system (Agilent 1260 HPLC) connected to Superdex 200 Increase 3.2/300 column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2024Quote: All protein expressions were carried out in BL21-CodonPlus (DE3)-RIL (Agilent Technologies). Cell lysis was performed by microfluidizer (Microfluidics ...
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bioRxiv - Immunology 2024Quote: ... and blocked for 1h at room temperature (Dako Protein block, Agilent Technologies, USA). Samples were incubated overnight at 4°C in anti-insulin antibody (1:500 Dako ...
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bioRxiv - Cell Biology 2024Quote: ... SSNA-1 proteins were expressed in Escherichia coli BL21(DE3) (Agilent Technologies Inc.) using TB media and induction with 0.4 mM IPTG overnight at 18°C ...
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bioRxiv - Developmental Biology 2020Quote: ... antibody staining was carried out using an anti-RFP antibody for 1h detected with EnVision HRP anti-rabbit secondary (Agilent) followed by incubation with Tyramide-conjugated Opal 570 (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako) in a dilution of 1:1500 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Bioengineering 2021Quote: ... or rabbit anti-CD206 antibody for 60 mins at room temperature Secondary antibodies donkey anti-mouse HRP and donkey anti-rabbit HRP (Dako) were applied for 30 mins at RT and samples were exposed DAB substrate (Dako ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane produced following transfer was probed with MAb214 primary antibody and then visualized with anti-mouse horseradish peroxidase secondary antibody (1:1000; Dako) for 1 h.
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X 5 mins in 1X PBS and incubated in secondary antibody diluted in DAKO antibody diluent reagent with background reducing components (DAKO) for 1 hour at room temperature with gentle agitation on a shaker (See Table S4 for antibody information) ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS + 0.1% Tween 20 and 5% dried skimmed milk powder and probed with relevant primary antibodies followed by horseradish peroxidase (HRP)-conjugated polyclonal goat anti-mouse and polyclonal goat anti-rabbit secondary antibodies (Dako). Membranes were treated with EZ-ECL (Geneflow ...
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bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved on 10–16% gradient SDS-PAGE under reducing conditions followed by immunoblotting for PMCA (primary antibody: 5F10 anti-pan-PMCA, Abcam, ab2825, secondary antibody: anti-mouse-HRP, Dako) and aSN (primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 nucleocapsid (EY-2A, a kind gift from Prof Alain Townsend) primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). Chemiluminescence substrate (West Dura ...
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted 1:100 or CD3 antibodies (Spring biosciences, Clone SP7, ref. M3070) diluted 1:200 in Antibody diluent solution (DakoCytomation). After several washes in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were immunostained with the primary antibody for 60 min and subsequently incubated with Dako EnVision HRP secondary antibody (Dako) for 30 min ...
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... The secondary antibodies used for western blotting were horseradish peroxidase (HRP)-conjugated antibody against mouse immunoglobulin G (IgG) and (HRP)-conjugated antibody against rabbit IgG from Dako.
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were incubated with the appropriate primary antibody diluted in TNB (for podoplanin) or Antibody Diluent (cat # S0809, Dako) (for VEGF-D or LYVE 1 ...