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Citations for Agilent :
551 - 600 of 627 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... samples were analyzed following formic acid quenching on an Agilent LC1290 Infinity II instrument coupled to a 6545 QTOF liquid chromatography–mass spectrometer (Agilent Technologies). A sample volume of 10 μl ...
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bioRxiv - Cancer Biology 2024Quote: ... AMP-derivatised fatty acids were detected as positive ions in QTOF-only mode on an Agilent 6560 IM-MS QTOF mass spectrometer (Agilent Technologies). Source conditions were as follows ...
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bioRxiv - Systems Biology 2024Quote: ... The digestion was quenched with 10% formic acid and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 6,000 cells per well in 80 µL of MEM media (MEM 10% FBS + 1% P/S + 1 mM Sodium Pyruvate + 2 mM Glutamax + 1% nonessential amino acids + 50 µg/mL uridine) in a Seahorse 96-well cell culture plate (Agilent, USA). After 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Gene expression analysis of Phago-MDMs and Phago+MDMs were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 28004) with two technical replicates for each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Genome-wide expression profiling was performed using the low-input QuickAmp labeling kit and human SurePrint G3 8×60K pangenomic microarrays (Agilent Technologies, Santa Clara, USA) as previously described (Merdrignac et al ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a refraction index (Surveyor RI Plus and Surveyor) on a 300 × 7.7 mm PL Hi-Plex H+ (8 μm particle size) column (Agilent Technologies, Santa Clara, CA, USA) and 4 × 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Evolutionary Biology 2024Quote: A phage display PAI-1 variant library was constructed on the I91L PAI-1 background using error prone PCR as previously described (7, 8) with the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) using primers that preserved the AscI and NotI restriction sites ...
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bioRxiv - Neuroscience 2021Quote: ... For ChR2-mediated optogenetic stimulation, blue light (473 nm, 6 Hz or 20 Hz) was produced using an arbitrary waveform generator (Agilent, 33220A) and a diode-pumped solid-state laser (Laserglow ...
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bioRxiv - Developmental Biology 2020Quote: ... RIN scores (RIN = 10 for all 6 samples) were measured on a BioAnalyzer 2100 using the RNA 6000 Nano assay (Agilent Technologies). Poly(A)-purified mRNA-seq library preparation was performed according to the TruSeq Stranded mRNA Reference Guide (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: Thermal denaturation experiments were performed using a qCHANGER 6/Cary60 (Quantum Northwest) interfaced to a Cary 60 UV-Visible spectrophotometer (Agilent Technologies) using a custom ADL script developed by Quantum Northwest to collect full spectra at each temperature as described previously ...
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bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
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bioRxiv - Molecular Biology 2024Quote: Complex formation between CtBP1 and CtBP2 variants and RAI2 variants was investigated on an analytical Superose 6 3.2/300 GL column (Cytiva, MA, USA) coupled to a 1260 Infinity HPLC system (Agilent, CA, USA). Proteins were dialyzed against the SEC buffer and injected onto a pre-equilibrated S6 column using an autosampler ...
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bioRxiv - Immunology 2024Quote: ... The product 6’-Cl-2’3’-cG(A)MP was purified using HPLC on a PFP Poroshell 120 column (Agilent Technologies, Inc.) with Acetonitrile/0.1% Formic Acid as the mobile phase ...
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bioRxiv - Biochemistry 2024Quote: ... sections were deparaffinized, dehydrated and subjected to antigen retrieval (20 min incubation at 98 °C in citrate buffer, pH 6 (Agilent Dako) for CD8 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded at 6 x 104 cells/well in a 24-well XF microplate (Agilent Technologies, Santa Clara, CA, USA). Specific inhibitors and uncouplers were prepared in XF assay media ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Evolutionary Biology 2021Quote: ... segetum to the synthetic mixture of C6-C10 straight chain acids were recorded on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID) (Agilent Technologies, USA) and an electroantennographic detector (EAD ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Systems Biology 2021Quote: ... detected with a diode array detector (DAD G7117A) and quantified following manufacturer’s guidelines (AdvanceBio Amino Acid Analysis, © Agilent Technologies, Inc. 2018). Standards ranging from 5 μM to 30 mM were used for the quantification of aspartate ...
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bioRxiv - Microbiology 2020Quote: Caprylic acid concentration was quantified by RP-HPLC following Herrera et al.37 using a liquid chromatographer (1220, Agilent Technologies; California, USA) equipped with an Eclipse XDB-C8 5 μm column (150 mm × 4.6 mm i.d.) ...
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bioRxiv - Cell Biology 2021Quote: ... The upper ether layer was collected and SCFA content was analyzed on an Agilent 7890B gas chromatography system with flame ionization detector using a high-resolution capillary column for detection of volatile acids (DB-FFAP, 30 m x 0.25 mm with 0.25 μm film thickness; Agilent Technologies, Santa Clara, CA). A standard solution containing 10 mM of acetic ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Biochemistry 2021Quote: ... The digestion and desalting (total time 3 min) were driven by 0.4% formic acid (FA) in water pumped by 1260 Infinity II Quaternary pump (Agilent Technologies, Waldbronn, Germany) at a flow rate of 100 μL·min−1 ...
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bioRxiv - Microbiology 2020Quote: Each ELV and MV preparation were reconstituted in 10 μl of 5% formic acid and injected onto a 50 mm 300 μm C18 trap column (Agilent Technologies, USA). The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of total RNA was measured on a Nanodrop device and the quality of the extracted nucleic acid was assessed using a Bio-analyzer 2100 (Agilent technologies, CA). Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentration of total RNA was measured using a Nanodrop spectrophotometer and the quality of the extracted nucleic acid was assessed on a Bio-analyzer 2100 (Agilent Technologies, CA). Microarray probes was synthetized by one cycle of RNA amplification in which molecules were labeled in an Affymetrix microarray station (Affymetrix ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-SΔ19 expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) by following the manufacturer’s instructions and using mutagenic oligonucleotides SaCoV2-K417T-F ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in hexane and fatty acids were separated with gas chromatography (8860 or 7890A GC system, Agilent Technologies, CA, USA) combined with mass spectrometry (5977B or 5975C Inert MS system ...
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bioRxiv - Physiology 2023Quote: ... High-resolution gas chromatography capillary column coated with 0.25µm film thickness was used (DB-FFAP) for the detection of volatile acids (Agilent Technologies, Santa Clara, CA). The oven temperature was 145°C and the FID and injection port were set to 225°C ...
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bioRxiv - Microbiology 2023Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cancer Biology 2022Quote: ... RAS double mutants with point-mutations in the 95th and 96th amino acid residues were generated by site-directed mutagenesis (Agilent Technologies #200521) using the primers listed in Supplementary Table 2 per manufacturer’s recommended PCR conditions ...
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bioRxiv - Biophysics 2024Quote: ... that is loaded onto a Waters HDX-Manager with a desalting flow of 0.23% aqueous formic acid solution (Solvent A) provided by an Agilent 1260 Infinity quaternary pump (Agilent Technologies, CA, USA) and a gradient flow comprised of Solvent A (0.23% aqueous FA solution ...
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bioRxiv - Microbiology 2024Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cell Biology 2024Quote: ... We mutated the Serine 69 to Alanine (69A) or aspartic acid (69D) using the QuickChange II Site-directed Mutagenesis kit (Agilent Technologies, #200523). The primer for mutating S69-A was ...
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bioRxiv - Genetics 2021Quote: ... and cultured chondrocytes of children (six girls and six boys) using catalog one-color microarrays (SurePrint G3 Human Gene Expression microarray, 8 × 60 k format; Agilent Technologies, Santa Clara, CA, USA). The data were analyzed using GeneSpring software (version 14.9 ...
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bioRxiv - Cancer Biology 2023Quote: ... the expression of 2,459 mature miRNAs was investigated by the microRNA microarray analysis using SurePrint G3 8×60K Human miRNA Microarray chips (AMADID 70156; Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the extraction of amanitins was performed using a vacuum manifold system (VacElut 6 Manifold Processing Station, Agilent Technologies, Santa Clara, CA, USA) with a 6 positions rack and OASIS® PRIME HLB cartridges (1 mL/30 mg ...
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bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: 384 well qPCR reaction plates for the multiplexed activation of endogenous genes (Figure 5 and 6) were set up using the Brilliant II SYBR master mix (Agilent cat #600828) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on the antibody used (HIF-1α, Dako Target Retrieval Solution pH 6; for BACH-1, Dako Target Retrieval Solution pH 9). Next ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... in addition to forward scattering and side scattering pulse area and width measurements (FSC-A, FSC-H, SSC- A, and SSC-H): cytoplasmic TdT/FITC (clone HT-6, Agilent/Dako, cat. F7139), CD56/PE (clone C5.9 ...
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bioRxiv - Pathology 2024Quote: ... and antigen retrieval was carried out using a sodium citrate buffer (10 mM, pH 6 or 9) on a PT Link system (DAKO, CA, USA). Endogenous peroxidase blockade was done using 3% hydrogen peroxide (Millipore ...
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bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...