Labshake search
Citations for Agilent :
5751 - 5800 of 8574 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and the cfDNA quality was assessed by the Fragment Analyzer High Sensitivity DNA Kit (Agilent, Santa Clara, California, USA).
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bioRxiv - Immunology 2024Quote: ... Library concentration and fragment sizes were evaluated with the Agilent High Sensitivity DNA kit (Cat. No. 5067-4626; Agilent). Libraries were sequenced on an Illumina NovaSeq 6000 sequencer (2x150 pb ...
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bioRxiv - Biochemistry 2023Quote: The F32A mutants of WT and Δ20-ACA8 were created using a QuikChange site directed mutagenesis kit (Agilent technologies) and the following primers:
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... was used for the RNA quality control with a microfluidic chip specific for RNA (Agilent RNA 6000 Nano Kit). Then 1 μg of qualified RNA samples in RNase-free ddH2O was used for library preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to remove genomic DNA contamination before cDNA was synthesised using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent) using Oligo DT ...
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bioRxiv - Genomics 2023Quote: ... and its quality was assessed using the Agilent Fragment analyzer 5200 with the DNA (50kb) kit (Agilent, DNF-467). Next ...
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bioRxiv - Biophysics 2024Quote: ... where residues I3 and I5 were replaced with alanine using the Quick-Change Lightning kit following the manufacturer’s instructions (Agilent). Following mutagenesis ...
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bioRxiv - Bioengineering 2024Quote: ... Error-prone PCR reactions were carried out following the protocol provided by GeneMorph II Random Mutagenesis Kit (200550, Agilent). Primers were designed to flank residue 1 to 119 of the binder ...
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bioRxiv - Immunology 2024Quote: ... RNA quantity and purity analysis was done using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Alanine mutagenesis of the Survivin sequence (pGST-Survivin) was carried out using the QuikChangeR Site-Directed Mutagenesis Kit (Stratagene). Plasmid encoding GFP-Survivin D70A/D71A (pGFP-Survivin D70A/D71A ...
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bioRxiv - Cell Biology 2024Quote: Oxygen Consumption Rates (OCR) were measured using the Seahorse XF Cell Mito Stress Test Kit (Agilent, Cat. 103708-100) with a Seahorse XFe96 analyzer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... The CDKN1B Serine 10 to alanine mutant reporter was cloned using the Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Genetics 2024Quote: ... Quality control for the libraries was performed using the Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). Libraries were sequenced using the Illumina Novaseq 6000 system with 2 × 150 paired-end kits ...
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bioRxiv - Cancer Biology 2023Quote: ... 154 and 175 of Twist1 in the pMPB3 vector were performed using the QuikChange XL site-directed mutagenesis kit accorinding to the manufacturer’s instructions (Stratagene), using the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Genetics 2023Quote: ... We measured library fragment sizes on the Agilent Tapestation using the Agilent High Sensitivity DNA 1000 kit (Agilent Technologies) and quantified library concentration using the Qubit IX dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was performed using the streptavidin-biotin-peroxydase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Immunology 2022Quote: ... The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200 ...
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bioRxiv - Microbiology 2023Quote: ... extracted RNA quality and concentration were measured using the Bio- analyser RNA Pico kit (Agilent, Cat. No. 5067-1513). Library preparation and sequencing were performed by the Oxford Genomics Centre (https://www.well.ox.ac.uk/ogc/) ...
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bioRxiv - Molecular Biology 2023Quote: All mutagenesis reactions were carried out using the QuikChange II Site-Directed or Lightning MultiSite-Directed Mutagenesis Kits (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... The concentration and quality of RNA samples from day 70 was assessed using a Bioanalyzer RNA kit (Agilent Technologies). Total RNA was prepped with the NuGEN Universal with AnyDeplete Human Ribo kit from ∼100 ng of total RNA input ...
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bioRxiv - Immunology 2023Quote: ... site-directed mutagenesis using specific primers was performed based on the QuikChange site-directed mutagenesis Kit II (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: EEF1A2 mutations in both the CMV-NGFP-EEF1A2-Neo vector and the pcDNA3.1-CMV-Flag-EEF1A2 vector were generated using the QuikChange II site directed mutagenesis kit (Agilent) according to the manufacturers protocol using the following primers.
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bioRxiv - Cell Biology 2023Quote: ... Mutations were inserted by site-directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA libraries were constructed using TruePrep DNA Library Prep Kit V2 for Illumina (TD503-02, Vazyme) following the manufacturer’s instructions and quantified by Agilent Bioanalyzer 2100 system ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 TPS was determined using the Dako PD-L1 IHC 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA), which is approved by the US Food and Drug Administration.
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using DNA Kit on the Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Genomics 2023Quote: ... Fragment size was controlled by capillary electrophoresis with the DNA high sensitivity kit and a Bioanalyzer 2100 (Agilent Technologies). DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the secondary antibody-horseradish peroxidase (HRP) complex from the REAL EnVision detection kit (Dako #K5007) for 1h at RT ...
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Biochemistry 2023Quote: ... For generation of single point LIR mutants and ΔLIR BAG3 the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) was utilized according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Concentration and quality of the synthesised cDNA were assessed using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). cDNA was successfully amplified from the suspension containing 8,000 nuclei however cDNA prepared from the suspension containing 8,000 cells was undetectable and therefore library preparation was discontinued at this step ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.2 kb) 20 was labelled by [a-32P]-dCTP by Prime-It II Random Primer Labeling Kit (Agilent Technologies) and hybridized with Hybond-N ...
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... λgt10 phage vectors in genomic DNA were excised using commercial packaging extract kits (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies, Inc., Cat. No. 5067-4626). Libraries were sequenced as paired-end 150×150bp on a NovaSeq® 6000 System (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5582, 5067-5583). Sequencing was done using a HiSeq 2500 (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Genomics 2023Quote: ... and size distribution was determined using the high sensitivity DNA kit on the Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pooled libraries were sequenced on the Illumina NovaSeq (paired-end 150 ...