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Citations for Agilent :
5701 - 5750 of 8606 citations for Estriol ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Biochemistry 2023Quote: ... For generation of single point LIR mutants and ΔLIR BAG3 the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) was utilized according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Concentration and quality of the synthesised cDNA were assessed using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). cDNA was successfully amplified from the suspension containing 8,000 nuclei however cDNA prepared from the suspension containing 8,000 cells was undetectable and therefore library preparation was discontinued at this step ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.2 kb) 20 was labelled by [a-32P]-dCTP by Prime-It II Random Primer Labeling Kit (Agilent Technologies) and hybridized with Hybond-N ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... λgt10 phage vectors in genomic DNA were excised using commercial packaging extract kits (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies, Inc., Cat. No. 5067-4626). Libraries were sequenced as paired-end 150×150bp on a NovaSeq® 6000 System (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5582, 5067-5583). Sequencing was done using a HiSeq 2500 (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Genomics 2023Quote: ... and size distribution was determined using the high sensitivity DNA kit on the Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pooled libraries were sequenced on the Illumina NovaSeq (paired-end 150 ...
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bioRxiv - Biochemistry 2022Quote: AtCOSY mutants were generated according to the protocol described in the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using plasmid pHis8-4b::AtCOSY as the template and the primer sequences in Supplementary Table 5 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quality was assessed using an Agilent 2100 bio-analyzer and the RNA 6000 pico kit (Agilent 5067-1513). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The TEAD-binding defective HPV8 E6 K136N mutant was made using the QuikChange II site-directed mutagenesis kit (Agilent). Nuclear/cytoplasmic fractionation was performed using the previously published Rapid ...
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bioRxiv - Biochemistry 2023Quote: ... The C121S ΔPTPN1 mutant was generated from the ΔPTPN1 vector using a QuikChange II Site Directed Mutagenesis kit (Agilent), and the mutant sequence verified by whole plasmid sequencing (Azenta) ...
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bioRxiv - Biochemistry 2023Quote: ... Library quality was assessed using an Agilent Bioanalyzer 2100 and the Agilent-1000 DNA kit (Agilent Cat# 5067-1504), and libraries were quantified using the Qubit dsDNA Quantitation ...
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bioRxiv - Biophysics 2023Quote: ... 104 and 140) cDNA in pET-7 vector was modified by site directed mutagenesis (QuikChange Lightning kit; Agilent Technologies) to introduce one of four single cysteine mutants (T26C ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA integrity was assessed using a microcapillary electrophoresis (TapeStation 4200, G2991BA) with RNA ScreenTape kit (Agilent Technologies, #5067-5576).
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bioRxiv - Cancer Biology 2023Quote: The mitochondrial function of MAFs was measured with a Seahorse XF Cell Mito Stress Test Kit (103015-100, Agilent) using a Seahorse XF96 Extracellular Flux Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: Site directed mutagenesis was performed on the pEGF-N2-DHRS7 vector according to the manufacturer’s recommendations using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) with primers designed using the Agilent on-line tool (https://www.agilent.com/store/primerDesignProgram.jsp).
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bioRxiv - Neuroscience 2023Quote: ... Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced in two lanes on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Genetics 2023Quote: ... Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518, Agilent Technologies), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Introduction of specific mutations in the SOSIP Envs was performed using QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) according to manufacturer’s instructions and verified by sequencing analysis (Eton Bioscience ...
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bioRxiv - Genomics 2023Quote: ... and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513). 100 ng of total RNA was quantified using the Qubit RNA High Sensitivity Assay (ThermoFisher Q32852 ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed on an Agilent 2100 Bioanalyzer using the RNA 6000 nano kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The average library size for the pool was determined using an Agilent DNA D1000 Tape Station kit (Agilent #5067).
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bioRxiv - Cancer Biology 2023Quote: ... their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). Libraries were pooled and sequenced with Illumina technology with paired-end reads of 50bp length.
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Biophysics 2023Quote: ... The Pro283Ser amino substitution was introduced to this vector via the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) as previously described (13) ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... These mutations were introduced into pJW1 by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies, Cat #200523) to make pJW1-KR>DD and pJW1-KR>AA ...
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bioRxiv - Cell Biology 2024Quote: ... Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotenone/Antimycin A was measured using the mitochondrial stress test kit (Agilent, USA). The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cancer Biology 2024Quote: ... Blood T cell fractions were estimated using TcellExTRECT using exons from the corresponding capture kit (Agilent custom V2 Exome) with a median coverage threshold set to five.
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bioRxiv - Physiology 2024Quote: ... for 24h then incubated in the Seahorse XF media (+/- 0.25mM succinate) and processed using the Cell Mito Stress Test Kit (#103015 Agilent).
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bioRxiv - Biochemistry 2024Quote: ... MicroRNAs (miRs) were polyadenylated and then re-verse-transcribed using the miR 1st-Strand cDNA Synthesis Kit (Agilent Technologies) according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... and the quality of the libraries was checked with a 2100 Bioanalyzer with a high-sensitivity DNA kit (Agilent). Libraries were sequenced using a P3 flow cell on a NextSeq2000 in 100 bp paired-end mode (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were run on an Agilent 2100 bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent, Diegem, Belgium). The bioanalyzer electropherograms were analyzed by Agilent 2100 Expert Software to determine the RNA quantity and quality ...