Labshake search
Citations for Agilent :
5551 - 5600 of 6226 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fragment size distributions of tagmented and dual indexed libraries were analyzed by capillary gel electrophoresis using a Bioanalyzer High Sensitivity DNA kit (50067-4626, Agilent, USA). Up to 384 high quality libraries with size distribution between 250-1000bp were pooled using equal molar quantities ...
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bioRxiv - Cell Biology 2023Quote: ... the integrity of DNAse I-treated RNA was determined using the Agilent RNA 6000 Pico Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RIN values > 4 was used for RT.
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bioRxiv - Cell Biology 2023Quote: ... TP53-missense mutations and protospacer adjacent motif (PAM) sequence mutation were introduced using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). A puromycin or neomycin resistance cassette was cloned into the BamHI site which was placed between left- and right-arm of the amplified and cloned DNA fragments ...
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bioRxiv - Genomics 2023Quote: ... The concentration and quality of the sample was evaluated with Qubit dsDNA HS kit and Tapestation D5000 Screen Tape (Agilent Technologies). The expected recovery was above 50 ng.
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Bioengineering 2023Quote: ... Specific site directed mutations on the S309 and CMAB0 antibody sequence was done using the Quick-change site directed mutagenesis kit II (Agilent technologies).
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The putative ESRRA binding site 1 was mutated using PCR-based site-directed mutagenesis and QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518) to obtain mutated constructs pGL3-Leptin mut 1-luc.
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bioRxiv - Microbiology 2023Quote: ... samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Systems Biology 2023Quote: ... The quality and quantity of all libraries were checked using Agilent’s Bioanalyzer 2100 and DNA 7500 kit (Agilent Technologies, 5067-1506).
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bioRxiv - Molecular Biology 2023Quote: ... The quality of RNA was evaluated using the Agilent 2100 Bioanalyzer with Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511). Libraries were prepared using the Illumina TruSeq Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina according to the manufacturer’s protocol and sequenced on an Illumina HiSeq 4000 (2× 75 bp).
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bioRxiv - Molecular Biology 2023Quote: ... The sequence was mutagenized to obtain the N-ter tag canonical variant 2 through the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521) using the following DNA primers ...
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bioRxiv - Genomics 2023Quote: ... and their size range was checked by capillary electrophoresis using with the DNA high sensitivity kit and a Bioanalyzer 2100 (Agilent Technologies). Libraries were diluted to 1 nM and pooled equimolarly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was measured using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA yield was determined using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... keeping in parity the Y320 residue of Gαi3 and Gαs the Y320E and Y320F mutants were generated using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The mutagenesis of the HDE-like motifs and PAM sequence within donor plasmid for HDR was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). pcDNA3.1(+)-ARRDC4-1 and pcDNA3.1(+)-ADCYAP1-2 expression vectors were constructed by cloning lnc-ARRDC4-1 and lnc-ADCYAP1-2 sequences to pcDNA3.1(+ ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Genomics 2023Quote: ... cDNA concentration was measured using Qubit dsDNA HS kit and the size distribution analyzed using Tapestation D5000 Screen Tape (Agilent Technologies).
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bioRxiv - Synthetic Biology 2023Quote: ... The average length of the DNA libraries was evaluated using Agilent Bioanalyzer 2100 and a High Sensitivity Kit (Agilent Technologies, USA) and the concentration of the DNA libraries was determined with a Qubit device (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies, Cat. No. / ID: 5067-4626). Libraries were sequenced on an Illumina NextSeq2000 in paired end mode with the read length of 50 nt and sequencing depth of 25 million reads per library ...
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bioRxiv - Cell Biology 2023Quote: ... Supplementary Table S3c) or for Ptgs1 generated by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). (Agilent ...
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bioRxiv - Genomics 2023Quote: We purified the DNA using AMPure beads (Beckmann Coulter, USA) and assessed quality using a Large Fragment Analysis Kit (Agilent, USA). The library was prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Integrity and concentrations of the RNA samples were determined using an Agilent 2100 bioanalyzer with RNA 6000 Nano Kit (Agilent, USA). Libraries for 6 samples were prepared using the Ion Total RNA-Seq Kit v2 according to the manufacturer’s protocol using 12-22 polyA-enriched RNA as input material ...
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bioRxiv - Cell Biology 2023Quote: The quantity and quality of RNA samples was assessed using the standard sensitivity RNA fragment analysis kit on Fragment Analyzer (Agilent Technologies). All samples had an RNA integrity number >8 and were deemed of sufficient quantity and quality for RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and the quality assessed with an Agilent 2100 Bioanalyzer with an RNA 144000 Nano Kit (Agilent technologies, Palo Alto, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... The remaining topology mutants of UBIAD1 were generated using the QuikChange® site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and pCMV-Myc-UBIAD1 as a template.
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bioRxiv - Cell Biology 2022Quote: ... were assessed using the XF96 Extracellular Analyzer as described in manufacturer’s instructions for the XF cell mito stress and the XF cell mito fuel flex test kits (Agilent Technologies, CA).Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... The total cellular ATP production rate is the sum of glycolytic and mitochondrial ATP (ATP Rate assay Kit; 103592-100, Agilent technologies). For ECAR analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were quality controlled using a 2100 Bioanalyzer system with Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc., 5067-4626) and quantified with Qubit™ 1X dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality of sequencing libraries was validated with a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626). The pooled sequencing libraries were quantified and sequenced on either the MiSeq (v2 or v2 nano ...
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bioRxiv - Biophysics 2022Quote: ... Amber codon mutagenesis in the selected positions were performed using the QuickChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, California, USA) following the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: PTEN monoubiquitination mutants PTEN-K13R and PTEN-K289R were generated from GFP-PTEN by site directed mutagenesis using QuickChange XL Site Directed Mutagenesis Kit (Agilent technologies). Table S1 includes the primer sequences.
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bioRxiv - Genomics 2022Quote: ... Quality check and quantification of total RNA were performed using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 Nano Kit (Agilent Technologies). Libraries were constructed from 500 ng total RNA using NEBNext Ultra II RNA - polyA+ (mRNA ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and the concentration and RNA Integrity Number (RIN) was determined with a RNA 6000 nano kit on 2100 Bioanalyzer instrument (Agilent Technologies). Real-time PCR was performed on samples in duplicate on an ABI 7900HT Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... The final libraries were then quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent, 5067-4626) and Qubit dsDNA HS Assay kit (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: ... Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE8×60K Microarray kit (design ID:028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Three Myc epitopes were then inserted before the stop codon into the three plasmids with the QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies) to get SLITRK3-WT-Myc ...
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bioRxiv - Genomics 2023Quote: ... All library quantifications were performed on a Qubit 3.0 fluorometer with High Sensitivity DNA Quantitation Assay kit according manufacturer’s recommendations (Agilent, ThermoFisher Scientific, Q32851). All library validations were performed on a 2100 Bioanalyzer with High Sensitivity DNA kit according to manufacturer’s recommendations (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... (3) RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR was performed using the 1-Step Brilliant II SYBR Green quantitative RT-PCR master mix kit (Agilent Technologies) on a Bio-Rad C1000 Thermal Cycler CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: ... The DNA from the released chromatin in the supernatant was purified and then quantified using Qubit dsDNA HS assay kit (Agilent Technologies). CUT&RUN libraries were constructed using NEBNext Ultra II DNA library preparation kit as described previously (Skene et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA libraries were constructed with the NEBNext® Ultra™ II FS DNA Library Prep Kit (Agilent, CA, US). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the fusion constructs were made from the appropriate backbone vector with the QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent Technologies). Reporters were sequenced from Genewiz before transfecting the parasites ...
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bioRxiv - Microbiology 2023Quote: ... AIP56D274S/D276-278S and AIP56D274N/D276-278N with or without V5-tag were generated with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, 200518) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and library profiles were assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Libraries were then sequenced on an Illumina NovaSeq instrument (Illumina ...