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Citations for Agilent :
5501 - 5550 of 6515 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... An A to C mutation was made at base pair 219 within the CAT-I gene using the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent) to match the native E ...
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bioRxiv - Biophysics 2023Quote: ... A clone encoding tagless wild type PUS1 was then generated from PUS1D134A by converting alanine 134 back to wild type aspartate 134 using a QuickChange II XL kit and protocol (Agilent).
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bioRxiv - Cell Biology 2023Quote: ... The total RNA yield was measured with a Nanodrop and the RNA integrity values (RIN) were obtained with a Bioanalyzer 2100 (Pico RNA 6000 Kit, Agilent). The values obtained for each of the samples are shown in Supplementary Table S1.
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bioRxiv - Immunology 2023Quote: ... following the manufacturer’s instructions and were run on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit (Agilent Technologies) to ensure the proper range of cDNA length distribution ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg DNase-treated RNA was reverse transcribed using a cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), following the respective manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Point mutations in KCNJ1 were introduced into the resulting pRS415TEF1-ROMK1 vector using either two-step overlap extension mutagenesis (141) or site-directed mutagenesis with the QuikChange kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene, CA) (Yoshida et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... according to manufacturer’s instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA-enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... The mutagenic primers were designed using QuickChange primer design program at www.agilent.com/genomics/qcpd according to the guidelines mentioned in the manufacturer’s protocol (QuickChange Lightning Site-Directed Mutagenesis kit, Cat# 210518, Agilent Technologies). This was followed by mutant strand synthesis and transformation was performed as mentioned in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 50 μM 2-deoxy-glucose (2-DG) + 1μg/μL Hoechst (Seahorse XF Glycolysis Stress Test Kit, Agilent, 103020) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA integrity was evaluated with a Fragment Analyzer (Advanced Analytical Technologies) using the HS NGS Fragment Kit (DNF-467, Agilent). dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... UHOR_02675 active site mutants (Ribo1-ME80Q/D98N) were created from by using QuickChange XL Site-Directed Mutagenesis Kit from Agilent Technology according to manufactures instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and run on the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara CA, USA) to confirm amplicon length and purity ...
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bioRxiv - Genetics 2023Quote: ... PREPLs was cloned into the bacterial expression vector pVP68K and mutations were introduced using the QuikChange II site directed mutagenesis kit (Stratagene) and confirmed by direct Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit; Quiagen; 204145) performed with the MX3005P thermocycler (Stratagene).
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bioRxiv - Cell Biology 2024Quote: ... Point mutations in Cebpa p42 were introduced by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518). Primers used for single mutant exchange were ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... One microgram or more of mouse genomic DNA from each sample was analyzed by whole exome sequencing using the SureSelectXT Mouse All Exon kit (Agilent), followed by next generation sequencing using the NovaSeq 6000 S4 flow cell (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... with the NucleoBond HMW DNA kit (Macherey Nagel, Düren, Germany) and DNA quality was assessed by capillary electrophoresis (Agilent FEMTOpulse). For PacBio library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The Lgmn mutation leading to an inactive LGMN C191A variant40,68 was introduced by site-directed mutagenesis using the QuickChange Site-Directed Mutagenesis Kit (# 200519, Agilent Technologies) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... The integrity of the RNA was tested on a Bioanalyzer 2100 using the Prokaryote Total RNA Nano kit (Agilent Technologies) and RNA Integrity Numbers (RIN ...
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bioRxiv - Genomics 2023Quote: ... and resulting size distribution was assessed with the High Sensitivity (HS) Large fragment kit run on the Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
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bioRxiv - Biochemistry 2023Quote: ... The NopD point mutants constructs were generated by different primers and were created by the QuickChange site-directed mutagenesis kit (Stratagene). All primers are shown in the Supplementary Table1 ...
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Bioengineering 2023Quote: ... Quality assessment of the isolated RNAs from each condition was conducted using the RNA 6000 Pico Kit and the 2100 Bioanalyzer Systems (Agilent), while quantification was carried out using the Qubit system (Invitrogen) ...
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bioRxiv - Systems Biology 2023Quote: Oxygen consumption rate was measured with the Seahorse XFe96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent), or using High-Resolution Respirometry (Oxygraph-2K ...
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bioRxiv - Cancer Biology 2023Quote: ... The size distribution and quality of the libraries were evaluated using a high sensitivity DNA kit (Agilent Technologies #5067-4626). Quantification of libraries was carried out with the Quant-iT DNA Assay Kit ...
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bioRxiv - Genomics 2023Quote: ... and the RNA integrity number (RIN) measured using the standard Agilent RNA 6000 Nano Kit Protocol and BioAnalyzer (Agilent Technologies). Extractions <7 RIN were not sequenced ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using the NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified with the Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The library size distribution was determined using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit (5067-4626, Agilent). We next used the SureSelect XT HS reagent Kits (5190-9685 and 5191-6686 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated by RNeasy Mini or RNeasy Plus Micro Kit and controlled for quality with a 2100 Bioanalyzer (Agilent) prior to cDNA libraries construction ...
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Biochemistry 2023Quote: RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-8000 (Nanodrop Technologies) and RNA integrity and concentration were assessed using a Fragment Analyzer SS-RNA kit (Agilent). Per sample ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... labeled cRNA was prepared from 0.1 micro-g Total RNA using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and assessed on a BioAnalyzer 2100 (Agilent) for library quantification and quality control ...
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bioRxiv - Cancer Biology 2023Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). ChIP was performed using IP-Star® Compact Automated System (Diagenode Cat# B03000002 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were washed twice with PBS-Tween and incubated for 30 minutes at room temperature with the Rabbit/Mouse EnVision kits (K4001/K4007, Dako). Slides were counterstained with hematoxylin and ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was amplified and Cy3-labeled by using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis of this clone was conducted to generate a genomic clone of NRL5P335L using the QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Final libraries were size-selected on Fragment Analyzer using the high-sensitivity Genomic Fragment Analyzer Kit (Agilent, Santa Clara, USA), quantified using the Fluoroskan plate fluorometer (Thermo Fisher Massachusetts ...
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bioRxiv - Microbiology 2024Quote: To detect changes in neutralisation potency single point mutations were introduced into env encoding plasmids for PV production using the QuikChange Lightning Site-Directed Mutagenesis (SDM) kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned up using the Takara Nucleospin kit per manufacturers protocol and concentration and size range assessed using the D5000 Screentape (Agilent). 200fmol of each sample was barcoded and subject to library prep using the Native Barcoding Ligation Kit with V14 chemistry (SQK-NBD-114-24 ...
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bioRxiv - Immunology 2023Quote: DNA whole exome libraries of B16-F10 cell line and C57BL/6JOlaHsd germline sample were prepared with Agilent Mouse All Exon kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... TamA G271C and G574C substitutions were introduced into pXW48 using the QuikChange Site-Drected Mutagenesis Kit (Agilent catalog number 200524). Plasmids that encode bamABCDE8His-bamB (pYG120 ...