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Citations for Agilent :
501 - 550 of 1961 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Peptides were acidified with trifluoroacetic acid (TFA) to lower the pH below 3 and desalted on reversed phase (RP) C18 OMIX tips (Agilent). The tips were first washed 3 times with 100 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Molecular Biology 2024Quote: ... and separated on a 50-cm reversed phase column packed in-house (Poroshell EC-C18, 2.7 μm, 50 cm × 75 μm; Agilent Technologies). Proteome samples were eluted over a linear gradient of a dual-buffer setup with buffer A (0.1% FA ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µg of lysate were digested and fractionated into six fractions using an Agilent AssayMAP Bravo equipped with Reversed Phase RP-S cartridge (Agilent). The “Protein Sample Prep WorkBench Fractionation application” was used with default settings ...
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bioRxiv - Neuroscience 2023Quote: ... with a Poroshell 120 SB-C18 reverse phase column (3.0x100 mm, 2.7 μm, particle size, Agilent Technologies, Santa Clara, CA) temperature-controlled at 25 LJ ...
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bioRxiv - Microbiology 2023Quote: ... Solid phase extraction of peptides was done using equilibrated C18 pipette tips (Bond Elut OMIX 100 µl pipette tips, Agilent). The samples are aspirated for 10 cycles for maximum binding efficiency and then eluted in LC-MS/MS vials with 100 µl of 0.1% TFA in water:acetonitrile (at a ratio of 30:70 (v/v)) ...
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bioRxiv - Plant Biology 2023Quote: ... and portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were analysed with solid phase microextraction (SPME) GC-MS (Agilent 7890 B and 5977 B MSD, Agilent, Inc.) with a Gerstel multipurpose sampler (MPS ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were analysed with solid phase microextraction (SPME) GC-MS (Agilent 7890 B and 5977 B MSD, Agilent, Inc.) with a Gerstel multipurpose sampler (MPS ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Biochemistry 2023Quote: Each mitochondrial rRNA was purified by using reversed-phase LC through a PLRP-S 4000Å column (4.6 × 150 mm, 10 μm, Agilent Technologies). After applying the mixture of rRNAs from the purified ribosome or total mitochondrial RNA to the column ...
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bioRxiv - Systems Biology 2024Quote: ... before reversed-phase separation on a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18, 2.7 µm, Agilent Technologies) using an increasing acetonitrile gradient ranging from 4 – 80% over 120 min including column wash and equilibration at a flow rate of 250 nL/min ...
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bioRxiv - Biochemistry 2022Quote: Purity of the wild-type or mutant FAM237A peptides was analyzed by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technologies). The FAM237A peptides were eluted from the C18 reverse-phase column by an alkaline acetonitrile gradient composed of alkaline solvent A and B [15] ...
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bioRxiv - Biochemistry 2022Quote: ... to remove their N-terminal 6×His-tag according to our previous procedure [3,18] and purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm; Agilent Technologies). For those C4ORF48-fused LEAP2 precursors ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded mature LEAP2s were then purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm; Agilent Technologies). Purity of the mature LEAP2s was analyzed by HPLC and their identity was confirmed by electrospray mass spectrometry.
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bioRxiv - Biochemistry 2022Quote: ... peptides were desalted by solid-phase extraction C18 columns (Sep-Pak, Waters) and fractionated into 22 fractions using an Agilent 1200 HPLC pump system (Agilent) coupled to a strong cation exchange (SCX ...
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bioRxiv - Biochemistry 2022Quote: ... The mature [S3C]UAG was eluted from an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm; Agilent Technologies) by an acetonitrile gradient ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide fractions were eluted from a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm; Agilent Technologies) by an acetonitrile gradient ...
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bioRxiv - Biophysics 2022Quote: ... The separation of samples was performed by 50 cm reverse-phase column (in-house packed with Poroshell 120 EC-C18, 2.7 μm, Agilent Technologies) with 180 min gradient ...
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bioRxiv - Cell Biology 2023Quote: ... Solid phase extraction of peptides was performed using C18 reversed phase sorbent containing 100 μl pipette tips Bond Elut OMIX 100 μl C18 tips (Agilent, Santa Clara ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrate was injected into a C18 reversed-phase column (4.6 mm×150 mm, 5 μm particle size, Agilent, USA) in a Thermo-Fisher Ultimate 3000 separation module equipped with a DAD-3000 Diode Array Detector ...
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bioRxiv - Immunology 2024Quote: ... We performed the HPLC with a normal phase Zobax Sil (5 μm, 4.6 × 150 mm) column (Agilent, Santa Clara, CA). Isocratic chromatographic separation was achieved with 10% ethyl acetate/hexane at a flow rate of 1.4 ml/min ...
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bioRxiv - Cell Biology 2024Quote: ... The HPLC conditions employed two normal-phase Zorbax Sil (5 μm, 4.6 × 150 mm) columns (Agilent, Santa Clara, CA, USA), connected in series within the Multicolumn Thermostat compartment ...
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bioRxiv - Physiology 2024Quote: The mixture of labeled peptides was subjected to reverse-phase LC fractionation using an Agilent 1200 Series HPLC (Agilent, USA) to obtain 24 fractions then fractions were combined (1 and 13 ...
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bioRxiv - Plant Biology 2024Quote: ... Thawed samples were centrifuged at 13000 g for 5 min at 4°C and the resulting aqueous phase was then analyzed by high-performance liquid chromatography (HPLC) in an Agilent 1260 Infinity system (Agilent) using a Metrosep A Supp 5 – 150/4.0 column (Metrohm) ...
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bioRxiv - Biochemistry 2024Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 x 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Plant Biology 2024Quote: ... Portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system and the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo) ...
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bioRxiv - Molecular Biology 2024Quote: 100-mer gRNAs were chemically synthesized under solid phase synthesis conditions by a commercial supplier (Agilent or Integrated DNA Technologies) with end-modifications as well as heavy 2’-O-methylribosugar modification as previously described26 ...
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bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
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bioRxiv - Biochemistry 2024Quote: ... the dried labeled sample was dissolved in 20 mM ammonium hydroxide (pH 10.8) and subjected to reversed-phase high pH fractionation using an Agilent 1260 Bioinert HPLC system (Agilent Technologies) over a 1.0 x 150 mm C18 column (XBridge Peptide BEH C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples were run through a 24-minute reversed-phase chromatography ZORBAX extend-C18 column (1.8 μm, 2.1mm × 150mm, 759700-902, Agilent, California, USA) combined with a guard column (1.8 μm ...
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bioRxiv - Biochemistry 2024Quote: ... Subsequent purification through reverse phase High-Performance Liquid Chromatography (HPLC) with a Zorbax Stable Bond 300 C18 column from Agilent using a linear gradient of 10 % - 90 % solvent B over 60 min was done ...
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bioRxiv - Biochemistry 2024Quote: ... TBDMS-derivatives were prepared for GC-MS with MOX and TBDMS as described previously.81,82 GC-MS analyses were performed on an Agilent 7890B GC system equipped with a HP-5MS capillary column (30 m, 0.25 mm i.d., 0.25 µm-phase thickness; Agilent J&W Scientific), connected to an Agilent 5977A Mass Spectrometer operating under ionization by electron impact (EI ...
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bioRxiv - Biochemistry 2024Quote: ... were kept in a 5°C autosampler prior to analysis and injected onto a reverse- phase Zorbax SB-C18 column (150×3 mm, 5 μm particle size, Agilent, Santa Clara ...
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bioRxiv - Systems Biology 2024Quote: ... Offline fractionation of TMT-labeled peptides was performed using a high-pH reversed-phase gradient with a ZORBAXTM C18 column (Agilent, 250 mm length/4.6 mM inner diameter/3.5 µm particle size ...
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bioRxiv - Biochemistry 2024Quote: ... The mature CXCL17 peptide was eluted from a C18 reverse-phase column (Zorbax 300SB-C8, 9.4 × 250 mm, Agilent Technologies) by an acidic acetonitrile gradient and manually collected ...
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Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... analysis with an Agilent Quadrupole Time of Flight LC/MS (6545 LC/Q-TOF) with a reverse-phase column (Agilent Poroshell ...
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bioRxiv - Developmental Biology 2024Quote: Drosophila extracts were analyzed on an UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight) mass spectrometry Impact II (Bruker Daltonics, Bremen, Germany) interfaced with an Agilent 1290 Infinity II LC Systems (Agilent Technologies, Santa Clara, CA, USA). LC separation was performed using a Waters reversed-phase (RP ...
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bioRxiv - Microbiology 2020Quote: ... Ethylene and acetylene gas from the gas phase of the Hungate tubes were determined using a GC (model GC-7890, Agilent Technologies) equipped with a flame ionization detector (FID) ...
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bioRxiv - Biochemistry 2021Quote: ... protein samples were diluted to a final concentration of 5 µM with solvent A and then desalted on a reverse phase-C8 cartridge (Zorbax 300SB-C8, 5 µm, 300 µm ID 5 mm, Agilent Technologies) at a flow rate of 50 μl/min for 3 min with 100 % solvent A and subsequently eluted with 70 % solvent B for MS detection ...
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bioRxiv - Microbiology 2021Quote: GC-MS measurements were executed on an Agilent 7890A GC system equipped with a DB-5ms capillary column (30 m, 0.25 mm, 0.25 μm-phase thickness; Agilent J&W Scientific), connected to a Waters Quattro Micro Tandem Mass Spectrometer (GC-MS/MS ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were injected into a C18 reverse phase column (Atlantis T3, 2.1 × 150 mm, 3 µm; Waters) using HPLC (Agilent 1290 LC) at a flow rate of 0.15 ml/min with 5 mM ammonium formate for mobile phase A and 100% methanol for mobile phase B ...
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bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
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bioRxiv - Synthetic Biology 2021Quote: ... 100 μL of the upper organic phase was used for Gas Chromatography-Mass Spectrometry (GC-MS, 5977B GC/MSD, Agilent Technologies) analysis ...
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bioRxiv - Systems Biology 2021Quote: GC-MS measurements were executed on an Agilent 7890A GC system equipped with a DB-5ms capillary column (30 m, 0.25 mm, 0.25 μm-phase thickness; Agilent J&W Scientific), connected to a Waters Quattro Micro Tandem Mass Spectrometer (GC-MS/MS ...
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bioRxiv - Biophysics 2020Quote: ... Peptides were trapped in a trap column (OPTI-TRAP for peptides, Optimize Technologies) and separated on a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies) using a 27 min gradient of 8-40 % organic solvent (0.4 % formic acid in acetonitrile ...
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bioRxiv - Biophysics 2020Quote: ... a peptide trap column (OPTI-TRAP for peptides, Optimize Technologies) and a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies). Pepsin digestion was performed online at a flow rate of 75 µL/min (0.4 % formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were offline fractionated over a 90 min run into 96 fractions by high pH reverse-phase HPLC (Agilent 1260) using an Agilent Zorbax 300 Extend-C18 column (3.5-µm ...
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bioRxiv - Biochemistry 2020Quote: ... The 5 μl purified TMSiPhe incorporated β-arrestin1 protein was loaded onto a reverse phase column (30 0SB-C8, 2.1 x 50 mm, 3.5 μM particle) (Agilent Technologies, SantaClara, CA). The proteins were then eluted over a gradient ...
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bioRxiv - Zoology 2021Quote: High pH reversed-phase liquid chromatography was performed on an offline Agilent 1200 series HPLC (Agilent Technologies, Santa Clara, CA, USA). The combined labeled digests (1.65 mg ...
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bioRxiv - Neuroscience 2021Quote: ... Retinoid analyses were performed using normal phase Zorbax Sil (5 mm, 4.6 3 150mm) column (Agilent Technologies, Santa Clara, CA, USA). The retinoids were eluted by step-wise gradient starting with 0.5% ethyl acetate in hexane over 15 min ...